Matches in UGent Biblio for { <https://biblio.ugent.be/publication/665443#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B287387.
- aggregation creator B287388.
- aggregation creator B287389.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2009".
- aggregation format "application/pdf".
- aggregation hasFormat 665443.bibtex.
- aggregation hasFormat 665443.csv.
- aggregation hasFormat 665443.dc.
- aggregation hasFormat 665443.didl.
- aggregation hasFormat 665443.doc.
- aggregation hasFormat 665443.json.
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- aggregation isPartOf urn:issn:1754-2189.
- aggregation language "eng".
- aggregation title "Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology".
- aggregation abstract "Here we provide a protocol for engineering the N-glycosylation pathway of the yeast Pichia pastoris. The general strategy consists of the disruption of an endogenous glycosyltransferase gene (OCH1) and the stepwise introduction of heterologous glycosylation enzymes. Each engineering step results in the introduction of one glycosidase or glycosyltransferase activity into the Pichia endoplasmic reticulum or Golgi complex and consists of a number of stages: transformation with the appropriate GlycoSwitch vector, small-scale cultivation of a number of transformants, sugar analysis and heterologous protein expression analysis. If desired, the resulting clone can be further engineered by repeating the procedure with the next GlycoSwitch vector. Each engineering step takes similar to 3 weeks. The conversion of any wild-type Pichia strain into a strain that modifies its glycoproteins with Gal(2)GlcNAc(2)Man(3)GlcNAc(2) N-glycans requires the introduction of five GlycoSwitch vectors. Three examples of the full engineering procedure are provided to illustrate the results that can be expected.".
- aggregation authorList BK570703.
- aggregation endPage "70".
- aggregation issue "1".
- aggregation startPage "58".
- aggregation volume "4".
- aggregation aggregates 2936732.
- aggregation isDescribedBy 665443.
- aggregation similarTo nprot.2008.213.
- aggregation similarTo LU-665443.