Matches in UGent Biblio for { <https://biblio.ugent.be/publication/737874#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B381905.
- aggregation creator B381906.
- aggregation creator B381907.
- aggregation creator B381908.
- aggregation creator B381909.
- aggregation creator B381910.
- aggregation date "2003".
- aggregation format "application/pdf".
- aggregation hasFormat 737874.bibtex.
- aggregation hasFormat 737874.csv.
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- aggregation hasFormat 737874.doc.
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- aggregation hasFormat 737874.yaml.
- aggregation isPartOf urn:issn:1462-2912.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Validation of a more sensitive method for using spotted oligonucleotide DNA microarrays for functional genomics studies on bacterial communities".
- aggregation abstract "Spotted oligonucleotide microarrays potentially offer a wide scope of applications for microbial ecology, especially as they improve the flexibility of design and the specificity of detection compared to PCR product based microarrays. Sensitivity, however, was expected to be problematic, as studies with the more sensitive PCR-based cDNA microarrays indicate that only genes from populations contributing to more than 5% of the community DNA can be detected. We evaluated several parameters to increase sensitivity and then tested applicability for bacterial functional genomics. The optimal parameters were the use of 5'-C6-amino-modified 70-mers printed on CMT-GAPS II substrates at a 40 muM concentration combined with the use of Tyramide Signal Amplification labelling. This protocol allowed detection of single copy genes belonging to an organism contributing to 1% or more of the total community. To demonstrate its application, we detected the specific aromatic oxygenase genes in a soil community degrading polychlorinated biphenyls (PCBs). This increase in sensitivity is important if oligonucleotide microarrays are to be used for simultaneous monitoring of a range of functions performed by different microorganisms in the environment.".
- aggregation authorList BK690020.
- aggregation endPage "943".
- aggregation issue "10".
- aggregation startPage "933".
- aggregation volume "5".
- aggregation aggregates 737879.
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- aggregation similarTo j.1462-2920.2003.00490.x.
- aggregation similarTo LU-737874.