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- 8537879 classification A1.
- 8537879 date "2017".
- 8537879 language "eng".
- 8537879 type journalArticle.
- 8537879 hasPart 8554099.pdf.
- 8537879 subject "Biology and Life Sciences".
- 8537879 subject "Medicine and Health Sciences".
- 8537879 doi "10.3791/55398".
- 8537879 issn "1940-087X".
- 8537879 issue "123".
- 8537879 abstract "Bronchoalveolar Lavage (BAL) is an experimental procedure that is used to examine the cellular and acellular content of the lung lumen ex vivo to gain insight into an ongoing disease state. Here, a simple and efficient method is described to perform BAL on murine lungs without the need of special tools or equipment. BAL fluid is isolated by inserting a catheter in the trachea of terminally anesthetized mice, through which a saline solution is instilled into the bronchioles. The instilled fluid is gently retracted to maximize BAL fluid retrieval and to minimize shearing forces. This technique allows the viability, function, and structure of cells within the airways and BAL fluid to be preserved. Numerous techniques may be applied to gain further understanding of the disease state of the lung. Here, a commonly used technique for the identification and enumeration of different types of immune cells is described, where flow cytometry is combined with a select panel of fluorescently labeled cell surface-specific markers. The BAL procedure presented here can also be used to analyze infectious agents, fluid constituents, or inhaled particles within murine lungs.".
- 8537879 author 1F1D02F4-F0EE-11E1-A9DE-61C894A0A6B4.
- 8537879 author CF2B0452-BC44-11E3-BB4D-77A64B2F559A.
- 8537879 author F48175CA-F0ED-11E1-A9DE-61C894A0A6B4.
- 8537879 author F778B0A4-F0ED-11E1-A9DE-61C894A0A6B4.
- 8537879 dateCreated "2017-11-17T13:43:03Z".
- 8537879 dateModified "2024-07-09T12:43:30Z".
- 8537879 name "Bronchoalveolar lavage of murine lungs to analyze inflammatory cell infiltration".
- 8537879 pagination urn:uuid:9d8983cc-6aa6-405d-a726-9486354011bf.
- 8537879 sameAs LU-8537879.
- 8537879 sourceOrganization urn:uuid:4320be5d-af58-400e-92e0-ee15c57d3e90.
- 8537879 sourceOrganization urn:uuid:75b83e66-20ed-4abd-a1f1-b63f95bf0afd.
- 8537879 type A1.