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- aggregation classification "A1".
- aggregation creator B330551.
- aggregation creator B330552.
- aggregation creator B330553.
- aggregation creator person.
- aggregation date "2010".
- aggregation format "application/pdf".
- aggregation hasFormat 883350.bibtex.
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- aggregation hasFormat 883350.doc.
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- aggregation isPartOf urn:issn:0168-1656.
- aggregation language "eng".
- aggregation publisher "Elsevier Science".
- aggregation rights "I don't know the status of the copyright for this publication".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris".
- aggregation abstract "Yeast surface display is an efficient tool for isolating and engineering antibody fragments, both scFv and Fab. We describe the use of protein display on Pichia pastoris for the rapid selection of camelid antibodies composed only of heavy chains (nanobodies) from a library derived from a llama immunized with Green Fluorescent Protein. The library of nanobody-coding sequences was fused to the C-terminal part of the Saccharomyces cerevisiae alpha-agglutinin gene (SAG1) and expressed in glycoengineered P. pastoris. A high efficiency transformation protocol yielded a library of 5×107 clones. About 80% of the clones strongly expressed the nanobody fusion. Nanobody-displaying clones were rapidly enriched by fluorescence activated cell sorting (FACS), and GFP-specific nanobody-displaying clones were isolated and equilibrium dissociation constants (Kd) determined. This technology for displaying protein libraries on P. pastoris enables the isolation and engineering of antibody-derived molecules in a robust eukaryotic expression host.".
- aggregation authorList BK623324.
- aggregation endPage "98".
- aggregation issue "2".
- aggregation startPage "93".
- aggregation volume "145".
- aggregation aggregates 2937204.
- aggregation isDescribedBy 883350.
- aggregation similarTo j.jbiotec.2009.10.010.
- aggregation similarTo LU-883350.