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- Bimolecular_fluorescence_complementation abstract "Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.".
- Bimolecular_fluorescence_complementation thumbnail Final_copy.jpg?width=300.
- Bimolecular_fluorescence_complementation wikiPageExternalLink kerppola_lab_bifc.
- Bimolecular_fluorescence_complementation wikiPageExternalLink EmPresentation.pdf.
- Bimolecular_fluorescence_complementation wikiPageID "2851991".
- Bimolecular_fluorescence_complementation wikiPageRevisionID "600021638".
- Bimolecular_fluorescence_complementation hasPhotoCollection Bimolecular_fluorescence_complementation.
- Bimolecular_fluorescence_complementation subject Category:Protein_methods.
- Bimolecular_fluorescence_complementation subject Category:Protein–protein_interaction_assays.
- Bimolecular_fluorescence_complementation type Ability105616246.
- Bimolecular_fluorescence_complementation type Abstraction100002137.
- Bimolecular_fluorescence_complementation type Appraisal105733583.
- Bimolecular_fluorescence_complementation type Assay105738625.
- Bimolecular_fluorescence_complementation type BasicCognitiveProcess105701944.
- Bimolecular_fluorescence_complementation type Classification105732756.
- Bimolecular_fluorescence_complementation type Cognition100023271.
- Bimolecular_fluorescence_complementation type Know-how105616786.
- Bimolecular_fluorescence_complementation type Method105660268.
- Bimolecular_fluorescence_complementation type Process105701363.
- Bimolecular_fluorescence_complementation type Protein-proteinInteractionAssays.
- Bimolecular_fluorescence_complementation type ProteinMethods.
- Bimolecular_fluorescence_complementation type PsychologicalFeature100023100.
- Bimolecular_fluorescence_complementation comment "Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells.".
- Bimolecular_fluorescence_complementation label "Bimolecular fluorescence complementation".
- Bimolecular_fluorescence_complementation label "Bimolekulare Fluoreszenzkomplementation".
- Bimolecular_fluorescence_complementation sameAs Bimolekulare_Fluoreszenzkomplementation.
- Bimolecular_fluorescence_complementation sameAs m.086wn8.
- Bimolecular_fluorescence_complementation sameAs Q863465.
- Bimolecular_fluorescence_complementation sameAs Q863465.
- Bimolecular_fluorescence_complementation sameAs Bimolecular_fluorescence_complementation.
- Bimolecular_fluorescence_complementation wasDerivedFrom Bimolecular_fluorescence_complementation?oldid=600021638.
- Bimolecular_fluorescence_complementation depiction Final_copy.jpg.
- Bimolecular_fluorescence_complementation isPrimaryTopicOf Bimolecular_fluorescence_complementation.
