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- Cell-cycle_analysis abstract "Cell-cycle analysis is a method in cell biology that employs flow cytometry to distinguish cells in different phases of the cell cycle. Before analysis, the cells are permeabilised and treated with a fluorescent dye that stains DNA quantitatively, usually propidium iodide (PI). The fluorescence intensity of the stained cells at certain wavelengths will therefore correlate with the amount of DNA they contain. As the DNA content of cells duplicates during the S phase of the cell cycle, the relative amount of cells in the G0 phase and G1 phase (before S phase), in the S phase, and in the G2 phase and M phase (after S phase) can be determined, as the fluorescence of cells in the G2/M phase will be twice as high as that of cells in the G0/G1 phase.Cell-cycle anomalies can be symptoms for various kinds of cell damage, for example DNA damage, which cause the cell to interrupt the cell cycle at certain checkpoints to prevent transformation into a cancer cell (carcinogenesis). Other possible reasons for anomalies include lack of nutrients, for example after serum deprivation.Cell cycle analysis was first described in 1969 at Los Alamos Scientific Laboratory by a group from the University of California, using the Feulgen staining technique. The first protocol for cell-cycle analysis using propidium iodide staining was presented in 1975 by Awtar Krishan from Harvard Medical School and is still widely cited today.".
- Cell-cycle_analysis thumbnail 1D30_DNA_DAPI.png?width=300.
- Cell-cycle_analysis wikiPageExternalLink cell-division.
- Cell-cycle_analysis wikiPageExternalLink Introduction%20to%20Cell%20Cycle%20Analysis.pdf.
- Cell-cycle_analysis wikiPageExternalLink cellcyc.pdf.
- Cell-cycle_analysis wikiPageID "27382300".
- Cell-cycle_analysis wikiPageRevisionID "591455421".
- Cell-cycle_analysis subject Category:Biological_techniques_and_tools.
- Cell-cycle_analysis subject Category:Cell_biology.
- Cell-cycle_analysis subject Category:Cell_cycle.
- Cell-cycle_analysis subject Category:Flow_cytometry.
- Cell-cycle_analysis comment "Cell-cycle analysis is a method in cell biology that employs flow cytometry to distinguish cells in different phases of the cell cycle. Before analysis, the cells are permeabilised and treated with a fluorescent dye that stains DNA quantitatively, usually propidium iodide (PI). The fluorescence intensity of the stained cells at certain wavelengths will therefore correlate with the amount of DNA they contain.".
- Cell-cycle_analysis label "Cell-cycle analysis".
- Cell-cycle_analysis sameAs m.0bxzylj.
- Cell-cycle_analysis sameAs Q5058181.
- Cell-cycle_analysis sameAs Q5058181.
- Cell-cycle_analysis wasDerivedFrom Cell-cycle_analysis?oldid=591455421.
- Cell-cycle_analysis depiction 1D30_DNA_DAPI.png.
- Cell-cycle_analysis isPrimaryTopicOf Cell-cycle_analysis.
