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- DNA_laddering abstract "DNA laddering is a feature that can be observed when DNA fragments resulting from apoptotic DNA fragmentation are visualised after separation by gel electrophoresis. It was first described in 1980 by Andrew Wyllie at the University of Edinburgh Medical School.DNA laddering is a distinctive feature of DNA degraded by caspase-activated DNase (CAD), which is a key event during apoptosis. CAD cleaves genomic DNA at internucleosomal linker regions, resulting in DNA fragments that are multiples of 180–185 base-pairs in length. Separation of the fragments by agarose gel electrophoresis and subsequent visualisation, for example by ethidium bromide staining, results in a characteristic "ladder" pattern.While most of the morphological features of apoptotic cells are short-lived, DNA laddering can be used as final state read-out method and has therefore become a reliable method to distinguish apoptosis from necrosis.".
- DNA_laddering thumbnail Apoptotic_DNA_Laddering.png?width=300.
- DNA_laddering wikiPageID "4791660".
- DNA_laddering wikiPageRevisionID "591036288".
- DNA_laddering hasPhotoCollection DNA_laddering.
- DNA_laddering subject Category:Apoptosis.
- DNA_laddering subject Category:Biological_techniques_and_tools.
- DNA_laddering subject Category:Cell_biology.
- DNA_laddering subject Category:Electrophoresis.
- DNA_laddering subject Category:Laboratory_techniques.
- DNA_laddering subject Category:Programmed_cell_death.
- DNA_laddering type Ability105616246.
- DNA_laddering type Abstraction100002137.
- DNA_laddering type BiologicalTechniquesAndTools.
- DNA_laddering type Cognition100023271.
- DNA_laddering type Know-how105616786.
- DNA_laddering type LaboratoryTechniques.
- DNA_laddering type Method105660268.
- DNA_laddering type PsychologicalFeature100023100.
- DNA_laddering type Technique105665146.
- DNA_laddering comment "DNA laddering is a feature that can be observed when DNA fragments resulting from apoptotic DNA fragmentation are visualised after separation by gel electrophoresis. It was first described in 1980 by Andrew Wyllie at the University of Edinburgh Medical School.DNA laddering is a distinctive feature of DNA degraded by caspase-activated DNase (CAD), which is a key event during apoptosis.".
- DNA_laddering label "DNA laddering".
- DNA_laddering sameAs m.0cnhyt.
- DNA_laddering sameAs Q5205755.
- DNA_laddering sameAs Q5205755.
- DNA_laddering sameAs DNA_laddering.
- DNA_laddering wasDerivedFrom DNA_laddering?oldid=591036288.
- DNA_laddering depiction Apoptotic_DNA_Laddering.png.
- DNA_laddering isPrimaryTopicOf DNA_laddering.
