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- Dideoxynucleotide abstract "Dideoxynucleotides are chain-terminating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing. They are also known as 2',3' dideoxynucleotides, and abbreviated as ddNTPs (ddGTP, ddATP, ddTTP and ddCTP).The absence of the 3'-hydroxyl group means that, after being added by a DNA polymerase to a growing nucleotide chain, no further nucleotides can be added as no phosphodiester bond can be created based on the fact that deoxyribonucleoside triphosphates (which are the building blocks of DNA) allow DNA chain synthesis to occur through a condensation reaction between the 5' phosphate (following the cleavage of pyrophospate) of the current nucleotide with the 3' hydroxyl group of the previous nucleotide. The dideoxyribonucleotides do not have a 3' hydroxyl group, hence no further chain elongation can occur once this dideoxynucleotide is on the chain. This can lead to the termination of the DNA sequence. Thus, these molecules form the basis of the dideoxy chain-termination method of DNA sequencing, which was developed by Frederick Sanger in 1977.Dideoxynucleotides are useful in the sequencing of DNA in combination with electrophoresis. A DNA sample that undergoes PCR (polymerase chain reaction) in a mixture containing all four deoxynucleotides and one dideoxynucleotide will produce strands of length equal to the position of each base of the type that complements the type having a dideoxynucleotide present. That is, each nucleotide base of that particular type has a probability of being bonded to not a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation. Thus, if the sample then undergoes electrophoresis, there will be a band present for each length at which the complement of the dideoxynucleotide is present. It is now common to use fluorescent dideoxynucleotides such that each one of the four has a different fluorescence that can be detected by a sequencer; thus only one reaction is needed.".
- Dideoxynucleotide thumbnail Dideoxyadenosine_triphosphate.svg?width=300.
- Dideoxynucleotide wikiPageID "3291893".
- Dideoxynucleotide wikiPageRevisionID "604731525".
- Dideoxynucleotide hasPhotoCollection Dideoxynucleotide.
- Dideoxynucleotide subject Category:Deoxy_sugars.
- Dideoxynucleotide subject Category:Nucleotides.
- Dideoxynucleotide type Abstraction100002137.
- Dideoxynucleotide type Chemical114806838.
- Dideoxynucleotide type Compound114818238.
- Dideoxynucleotide type Ester114850483.
- Dideoxynucleotide type Material114580897.
- Dideoxynucleotide type Matter100020827.
- Dideoxynucleotide type Nucleotide114964590.
- Dideoxynucleotide type Nucleotides.
- Dideoxynucleotide type OrganicCompound114727670.
- Dideoxynucleotide type Part113809207.
- Dideoxynucleotide type PhysicalEntity100001930.
- Dideoxynucleotide type Relation100031921.
- Dideoxynucleotide type Substance100019613.
- Dideoxynucleotide comment "Dideoxynucleotides are chain-terminating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing.".
- Dideoxynucleotide label "Dideossiribonucleotide".
- Dideoxynucleotide label "Dideoxynucleotide".
- Dideoxynucleotide label "Didesoxinucleótido".
- Dideoxynucleotide label "Didesoxyribonukleosid-Triphosphate".
- Dideoxynucleotide label "Didésoxyribonucléotide".
- Dideoxynucleotide label "双脱氧核苷酸".
- Dideoxynucleotide sameAs Dideoxyribonukleotid.
- Dideoxynucleotide sameAs Didesoxyribonukleosid-Triphosphate.
- Dideoxynucleotide sameAs Didesoxinucleótido.
- Dideoxynucleotide sameAs Didésoxyribonucléotide.
- Dideoxynucleotide sameAs Dideossiribonucleotide.
- Dideoxynucleotide sameAs m.093qpk.
- Dideoxynucleotide sameAs Q421201.
- Dideoxynucleotide sameAs Q421201.
- Dideoxynucleotide sameAs Dideoxynucleotide.
- Dideoxynucleotide wasDerivedFrom Dideoxynucleotide?oldid=604731525.
- Dideoxynucleotide depiction Dideoxyadenosine_triphosphate.svg.
- Dideoxynucleotide isPrimaryTopicOf Dideoxynucleotide.
