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- Differential_centrifugation abstract "Differential centrifugation is a common procedure in microbiology and cytology used to separate certain organelles from whole cells for further analysis of specific parts of cells. In the process, a tissue sample is first homogenised to break the cell membranes and mix up the cell contents. The homogenate is then subjected to repeated centrifugations, each time removing the pellet and increasing the centrifugal force. Finally, purification may be done through equilibrium sedimentation, and the desired layer is extracted for further analysis.Separation is based on size and density, with larger and denser particles pelleting at lower centrifugal forces. As an example, unbroken whole cells will pellet at low speeds and short intervals such as 1,000g for 5 minutes. Smaller cell fragments and organelles remain in the supernatant and require more force and greater times to pellet. In general, one can enrich for the following cell components, in the separating order in actual application:Whole cells and nuclei;Mitochondria, lysosomes and peroxisomes;Microsomes (vesicles of disrupted endoplasmic reticulum); andRibosomes and cytosol.".
- Differential_centrifugation thumbnail Differentielle_zentrifugation.gif?width=300.
- Differential_centrifugation wikiPageExternalLink Sucrose_gradient_centrifugation.html.
- Differential_centrifugation wikiPageID "512768".
- Differential_centrifugation wikiPageRevisionID "603633178".
- Differential_centrifugation by "no".
- Differential_centrifugation hasPhotoCollection Differential_centrifugation.
- Differential_centrifugation label "Ultracentrifugation".
- Differential_centrifugation lcheading "Ultracentrifugation".
- Differential_centrifugation onlinebooks "no".
- Differential_centrifugation subject Category:Cell_biology.
- Differential_centrifugation subject Category:Centrifugation.
- Differential_centrifugation subject Category:Industrial_processes.
- Differential_centrifugation type Ability105616246.
- Differential_centrifugation type Abstraction100002137.
- Differential_centrifugation type Cognition100023271.
- Differential_centrifugation type Know-how105616786.
- Differential_centrifugation type LaboratoryTechniques.
- Differential_centrifugation type Method105660268.
- Differential_centrifugation type PsychologicalFeature100023100.
- Differential_centrifugation type Technique105665146.
- Differential_centrifugation comment "Differential centrifugation is a common procedure in microbiology and cytology used to separate certain organelles from whole cells for further analysis of specific parts of cells. In the process, a tissue sample is first homogenised to break the cell membranes and mix up the cell contents. The homogenate is then subjected to repeated centrifugations, each time removing the pellet and increasing the centrifugal force.".
- Differential_centrifugation label "Centrifugation analytique".
- Differential_centrifugation label "Dichtegradientenzentrifugation".
- Differential_centrifugation label "Differential centrifugation".
- Differential_centrifugation label "Ultracentrifugación".
- Differential_centrifugation label "Ультрацентрифугирование".
- Differential_centrifugation sameAs Dichtegradientenzentrifugation.
- Differential_centrifugation sameAs Ultracentrifugación.
- Differential_centrifugation sameAs Centrifugation_analytique.
- Differential_centrifugation sameAs Ultracentrifugação.
- Differential_centrifugation sameAs m.02k6r0.
- Differential_centrifugation sameAs Q2509459.
- Differential_centrifugation sameAs Q2509459.
- Differential_centrifugation sameAs Differential_centrifugation.
- Differential_centrifugation wasDerivedFrom Differential_centrifugation?oldid=603633178.
- Differential_centrifugation depiction Differentielle_zentrifugation.gif.
- Differential_centrifugation isPrimaryTopicOf Differential_centrifugation.
