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- ELISA abstract "The enzyme-linked immunosorbent assay (ELISA) (/ɨˈlaɪzə/, /ˌiːˈlaɪzə/) is a test that uses antibodies and color change to identify a substance. ELISA is a popular format of "wet-lab" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. Antigens from the sample are attached to a surface. Then, a further specific antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate.Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are aspecifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.Of note, ELISA can perform other forms of ligand binding assays instead of strictly "immuno" assays, though the name carried the original "immuno" because of the common use and history of development of this method. The technique essentially requires any ligating reagent that can be immobilized on the solid phase along with a detection reagent that will bind specifically and use an enzyme to generate a signal that can be properly quantified. In between the washes, only the ligand and its specific binding counterparts remain specifically bound or "immunosorbed" by antigen-antibody interactions to the solid phase, while the nonspecific or unbound components are washed away. Unlike other spectrophotometric wet lab assay formats where the same reaction well (e.g. a cuvette) can be reused after washing, the ELISA plates have the reaction products immunosorbed on the solid phase which is part of the plate, so are not easily reusable.".
- ELISA thumbnail Microtiter_plate.JPG?width=300.
- ELISA wikiPageExternalLink main.html.
- ELISA wikiPageExternalLink ZWxpc2Ega2l0cw==.html.
- ELISA wikiPageExternalLink elisa-animation.html.
- ELISA wikiPageExternalLink Page.asp?PageId=26&PageName=About+ELISA.
- ELISA wikiPageExternalLink elisaformats.
- ELISA wikiPageExternalLink ELISA.html.
- ELISA wikiPageExternalLink kb07an01.htm.
- ELISA wikiPageID "95162".
- ELISA wikiPageRevisionID "606661012".
- ELISA caption "A 96-well microtiter plate being used for ELISA.".
- ELISA hasPhotoCollection ELISA.
- ELISA meshid "D004797".
- ELISA name "ELISA".
- ELISA subject Category:Immunologic_tests.
- ELISA type Abstraction100002137.
- ELISA type Cognition100023271.
- ELISA type Experiment105798043.
- ELISA type HigherCognitiveProcess105770664.
- ELISA type ImmunologicTests.
- ELISA type Inquiry105797597.
- ELISA type ProblemSolving105796750.
- ELISA type Process105701363.
- ELISA type PsychologicalFeature100023100.
- ELISA type Thinking105770926.
- ELISA type Trial105799212.
- ELISA comment "The enzyme-linked immunosorbent assay (ELISA) (/ɨˈlaɪzə/, /ˌiːˈlaɪzə/) is a test that uses antibodies and color change to identify a substance. ELISA is a popular format of "wet-lab" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries.".
- ELISA label "ELISA (test immunoenzymatyczny)".
- ELISA label "ELISA".
- ELISA label "ELISA".
- ELISA label "ELISA".
- ELISA label "ELISA".
- ELISA label "ELISA".
- ELISA label "ELISA".
- ELISA label "Enzyme-linked Immunosorbent Assay".
- ELISA label "Méthode immuno-enzymatique ELISA".
- ELISA label "Иммуноферментный анализ".
- ELISA label "إليزا (المقايسة المناعية المرتبط بالإنزيم)".
- ELISA label "酶联免疫吸附试验".
- ELISA sameAs ELISA.
- ELISA sameAs Enzyme-linked_Immunosorbent_Assay.
- ELISA sameAs ELISA.
- ELISA sameAs ELISA.
- ELISA sameAs Méthode_immuno-enzymatique_ELISA.
- ELISA sameAs ELISA.
- ELISA sameAs ELISA.
- ELISA sameAs ELISA.
- ELISA sameAs 효소_결합_면역_침강_분석법.
- ELISA sameAs ELISA.
- ELISA sameAs ELISA_(test_immunoenzymatyczny).
- ELISA sameAs ELISA.
- ELISA sameAs m.0nk2z.
- ELISA sameAs Q332126.
- ELISA sameAs Q332126.
- ELISA sameAs ELISA.
- ELISA wasDerivedFrom ELISA?oldid=606661012.
- ELISA depiction Microtiter_plate.JPG.
- ELISA isPrimaryTopicOf ELISA.
