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- ELISPOT abstract "The enzyme-linked immunosorbent spot (ELISPOT) assay is a common method for monitoring immune responses in humans and other animals. It was developed by Cecil Czerkinsky in 1983.The ELISPOT assay is based on, and was developed from a modified version of the ELISA immunoassay. ELISPOT assays were originally developed to enumerate B cells secreting antigen-specific antibodies, and have subsequently been adapted for various tasks, especially the identification and enumeration of cytokine-producing cells at the single cell level. Simply put, at appropriate conditions the ELISPOT assay allows visualization of the secretory product of individual activated or responding cells. Each spot that develops in the assay represents a single reactive cell. Thus, the ELISPOT assay provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.By virtue of exquisite sensitivity of the ELISPOT assay, frequency analysis of rare cell populations (e.g., antigen-specific responses) which were not possible before are now relatively easy. This exceptional sensitivity is in part because the product is rapidly captured around the secreting cell: before it is either diluted in the supernatant, captured by receptors of adjacent cells, or degraded. This makes ELISPOT assays much more sensitive than conventional ELISA measurements. Limits of detection are below 1/100,000 rendering enumerate the actively producing cells. This allows much of the analysis process to be automated, and permits a greater level of accuracy than what can be achieved using manual inspection.".
- ELISPOT thumbnail ELISPOT-en.png?width=300.
- ELISPOT wikiPageExternalLink elispot-wells.html.
- ELISPOT wikiPageExternalLink elispot-animation.html.
- ELISPOT wikiPageExternalLink IUQ0Cr_shrestha.pdf.
- ELISPOT wikiPageExternalLink elispot-animation.
- ELISPOT wikiPageID "502455".
- ELISPOT wikiPageRevisionID "604486444".
- ELISPOT hasPhotoCollection ELISPOT.
- ELISPOT subject Category:Immunologic_tests.
- ELISPOT type Abstraction100002137.
- ELISPOT type Cognition100023271.
- ELISPOT type Experiment105798043.
- ELISPOT type HigherCognitiveProcess105770664.
- ELISPOT type ImmunologicTests.
- ELISPOT type Inquiry105797597.
- ELISPOT type ProblemSolving105796750.
- ELISPOT type Process105701363.
- ELISPOT type PsychologicalFeature100023100.
- ELISPOT type Thinking105770926.
- ELISPOT type Trial105799212.
- ELISPOT comment "The enzyme-linked immunosorbent spot (ELISPOT) assay is a common method for monitoring immune responses in humans and other animals. It was developed by Cecil Czerkinsky in 1983.The ELISPOT assay is based on, and was developed from a modified version of the ELISA immunoassay.".
- ELISPOT label "ELISPOT".
- ELISPOT label "ELISPOT".
- ELISPOT label "ELISPOT".
- ELISPOT label "ELISPOT".
- ELISPOT sameAs ELISPOT.
- ELISPOT sameAs ELISPOT.
- ELISPOT sameAs ELISPOT.
- ELISPOT sameAs m.02j0v_.
- ELISPOT sameAs Q731235.
- ELISPOT sameAs Q731235.
- ELISPOT sameAs ELISPOT.
- ELISPOT wasDerivedFrom ELISPOT?oldid=604486444.
- ELISPOT depiction ELISPOT-en.png.
- ELISPOT isPrimaryTopicOf ELISPOT.
