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- Eosin_methylene_blue abstract "Eosin Methylene Blue (EMB, also known as "Levine's formulation") is a slightly selective stain for Gram-negative bacteria. It is a blend of two stains, eosin and methylene blue in the ratio of 6:1. A common application of this stain is in the preparation of EMB agar, a differential microbiological medium, which slightly inhibits the growth of Gram-positive bacteria and provides a color indicator distinguishing between organisms that ferment lactose (e.g., E. coli) and those that do not (e.g., Salmonella, Shigella). Organisms that ferment lactose display "nucleated colonies" -- colonies with dark centers.This medium is important in medical laboratories by distinguishing pathogenic microbes in a short period of time.Rapid lactose fermentation produces acids, which lower the pH. This encourages dye absorption by the colonies, which are now colored purple-black.Lactose non-fermenters may increase the pH by deamination of proteins. This ensures that the dye is not absorbed. The colonies will be colorless. On EMB if E. coli is grown it will give a distinctive metallic green sheen (due to the metachromatic properties of the dyes, E. coli movement using flagella, and strong acid end-products of fermentation). Some species of Citrobacter and Enterobacter will also react this way to EMB.This medium has been specifically designed to discourage the growth of gram positive bacteria.EMB contains the following ingredients:peptone,Lactose,Dipotassium phosphate,Eosin Y (dye),Methylene blue (dye),Agar.There are also EMB agars that do not contain lactose.".
- Eosin_methylene_blue thumbnail Coli_levine.JPG?width=300.
- Eosin_methylene_blue wikiPageExternalLink 17.
- Eosin_methylene_blue wikiPageExternalLink eosin_methylene_blue_agar.php.
- Eosin_methylene_blue wikiPageExternalLink Eosin_Methylene_Blue_Agar_Modified.pdf.
- Eosin_methylene_blue wikiPageExternalLink details.asp?id=2553&Lang=.
- Eosin_methylene_blue wikiPageExternalLink product_info.php?products_id=10038.
- Eosin_methylene_blue wikiPageExternalLink 7103_PI.pdf.
- Eosin_methylene_blue wikiPageID "1610978".
- Eosin_methylene_blue wikiPageRevisionID "605415698".
- Eosin_methylene_blue hasPhotoCollection Eosin_methylene_blue.
- Eosin_methylene_blue subject Category:Microbiological_media.
- Eosin_methylene_blue subject Category:Microbiological_media_ingredients.
- Eosin_methylene_blue subject Category:Staining.
- Eosin_methylene_blue type Component103081021.
- Eosin_methylene_blue type Ingredient103570709.
- Eosin_methylene_blue type MicrobiologicalMediaIngredients.
- Eosin_methylene_blue type Object100002684.
- Eosin_methylene_blue type Part103892891.
- Eosin_methylene_blue type PhysicalEntity100001930.
- Eosin_methylene_blue comment "Eosin Methylene Blue (EMB, also known as "Levine's formulation") is a slightly selective stain for Gram-negative bacteria. It is a blend of two stains, eosin and methylene blue in the ratio of 6:1. A common application of this stain is in the preparation of EMB agar, a differential microbiological medium, which slightly inhibits the growth of Gram-positive bacteria and provides a color indicator distinguishing between organisms that ferment lactose (e.g., E.".
- Eosin_methylene_blue label "EMB寒天培地".
- Eosin_methylene_blue label "Eosin methylene blue".
- Eosin_methylene_blue label "Eosin-Methylen-Blau".
- Eosin_methylene_blue label "Eosina azul de metileno".
- Eosin_methylene_blue label "Eosina azul de metileno".
- Eosin_methylene_blue label "Pożywka Levine'a".
- Eosin_methylene_blue sameAs Eosin-Methylen-Blau.
- Eosin_methylene_blue sameAs Eosina_azul_de_metileno.
- Eosin_methylene_blue sameAs EMB寒天培地.
- Eosin_methylene_blue sameAs Pożywka_Levine'a.
- Eosin_methylene_blue sameAs Eosina_azul_de_metileno.
- Eosin_methylene_blue sameAs m.05gj7_.
- Eosin_methylene_blue sameAs Q1346346.
- Eosin_methylene_blue sameAs Q1346346.
- Eosin_methylene_blue sameAs Eosin_methylene_blue.
- Eosin_methylene_blue wasDerivedFrom Eosin_methylene_blue?oldid=605415698.
- Eosin_methylene_blue depiction Coli_levine.JPG.
- Eosin_methylene_blue isPrimaryTopicOf Eosin_methylene_blue.
