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• Fluorescence_correlation_spectroscopy abstract "Fluorescence correlation spectroscopy (FCS) is a correlation analysis of fluctuation of the fluorescence intensity. The analysis provides parameters of the physics under the fluctuations. One of the interesting applications of this is an analysis of the concentration fluctuations of fluorescent particles (molecules) in solution. In this application, the fluorescence emitted from a very tiny space in solution containing a small number of fluorescent particles (molecules) is observed. The fluorescence intensity is fluctuating due to Brownian motion of the particles. In other words, the number of the particles in the sub-space defined by the optical system is randomly changing around the average number. The analysis gives the average number of fluorescent particles and average diffusion time, when the particle is passing through the space. Eventually, both the concentration and size of the particle (molecule) are determined. Both parameters are important in biochemical research, biophysics, and chemistry.FCS is such a sensitive analytical tool because it observes a small number of molecules (nanomolar to picomolar concentrations) in a small volume (~1μm3). In contrast to other methods (such as HPLC analysis) FCS has no physical separation process; instead, it achieves its spatial resolution through its optics. Furthermore, FCS enables observation of fluorescence-tagged molecules in the biochemical pathway in intact living cells. This opens a new area, "in situ or in vivo biochemistry": tracing the biochemical pathway in intact cells and organs.Commonly, FCS is employed in the context of optical microscopy, in particular Confocal microscopy or two-photon excitation microscopy. In these techniques light is focused on a sample and the measured fluorescence intensity fluctuations (due to diffusion, physical or chemical reactions, aggregation, etc.) are analyzed using the temporal autocorrelation. Because the measured property is essentially related to the magnitude and/or the amount of fluctuations, there is an optimum measurement regime at the level when individual species enter or exit the observation volume (or turn on and off in the volume). When too many entities are measured at the same time the overall fluctuations are small in comparison to the total signal and may not be resolvable – in the other direction, if the individual fluctuation-events are too sparse in time, one measurement may take prohibitively too long. FCS is in a way the fluorescent counterpart to dynamic light scattering, which uses coherent light scattering, instead of (incoherent) fluorescence.When an appropriate model is known, FCS can be used to obtain quantitative information such as diffusion coefficients hydrodynamic radii average concentrations kinetic chemical reaction rates singlet-triplet dynamicsBecause fluorescent markers come in a variety of colors and can be specifically bound to a particular molecule (e.g. proteins, polymers, metal-complexes, etc.), it is possible to study the behavior of individual molecules (in rapid succession in composite solutions). With the development of sensitive detectors such as avalanche photodiodes the detection of the fluorescence signal coming from individual molecules in highly dilute samples has become practical. With this emerged the possibility to conduct FCS experiments in a wide variety of specimens, ranging from materials science to biology. The advent of engineered cells with genetically tagged proteins (like green fluorescent protein) has made FCS a common tool for studying molecular dynamics in living cells.".
• Fluorescence_correlation_spectroscopy thumbnail Fluorescence_correlation_spectroscopy_instrument_diagram.png?width=300.
• Fluorescence_correlation_spectroscopy wikiPageExternalLink j.sbi.2004.09.004.
• Fluorescence_correlation_spectroscopy wikiPageExternalLink index.htm.
• Fluorescence_correlation_spectroscopy wikiPageExternalLink imaging_approaches_correlation_microscopy.shtml.
• Fluorescence_correlation_spectroscopy wikiPageExternalLink classroom.
• Fluorescence_correlation_spectroscopy wikiPageExternalLink idl.
• Fluorescence_correlation_spectroscopy wikiPageID "2589751".
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• Fluorescence_correlation_spectroscopy hasPhotoCollection Fluorescence_correlation_spectroscopy.
• Fluorescence_correlation_spectroscopy subject Category:Fluorescence_techniques.
• Fluorescence_correlation_spectroscopy subject Category:Physical_chemistry.
• Fluorescence_correlation_spectroscopy subject Category:Scientific_techniques.
• Fluorescence_correlation_spectroscopy subject Category:Spectroscopy.
• Fluorescence_correlation_spectroscopy type Ability105616246.
• Fluorescence_correlation_spectroscopy type Abstraction100002137.
• Fluorescence_correlation_spectroscopy type Cognition100023271.
• Fluorescence_correlation_spectroscopy type FluorescenceTechniques.
• Fluorescence_correlation_spectroscopy type Know-how105616786.
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• Fluorescence_correlation_spectroscopy type PsychologicalFeature100023100.
• Fluorescence_correlation_spectroscopy type ScientificTechniques.
• Fluorescence_correlation_spectroscopy type Technique105665146.
• Fluorescence_correlation_spectroscopy comment "Fluorescence correlation spectroscopy (FCS) is a correlation analysis of fluctuation of the fluorescence intensity. The analysis provides parameters of the physics under the fluctuations. One of the interesting applications of this is an analysis of the concentration fluctuations of fluorescent particles (molecules) in solution. In this application, the fluorescence emitted from a very tiny space in solution containing a small number of fluorescent particles (molecules) is observed.".
• Fluorescence_correlation_spectroscopy label "Fluorescence correlation spectroscopy".
• Fluorescence_correlation_spectroscopy label "Fluoreszenzkorrelationsspektroskopie".
• Fluorescence_correlation_spectroscopy label "Spectroscopie de corrélation de fluorescence".
• Fluorescence_correlation_spectroscopy label "蛍光相関分光法".
• Fluorescence_correlation_spectroscopy sameAs Fluoreszenzkorrelationsspektroskopie.
• Fluorescence_correlation_spectroscopy sameAs Spectroscopie_de_corrélation_de_fluorescence.
• Fluorescence_correlation_spectroscopy sameAs 蛍光相関分光法.
• Fluorescence_correlation_spectroscopy sameAs 형광_상관_분광학.
• Fluorescence_correlation_spectroscopy sameAs m.07q3cm.
• Fluorescence_correlation_spectroscopy sameAs Q902601.
• Fluorescence_correlation_spectroscopy sameAs Q902601.
• Fluorescence_correlation_spectroscopy sameAs Fluorescence_correlation_spectroscopy.
• Fluorescence_correlation_spectroscopy wasDerivedFrom Fluorescence_correlation_spectroscopy?oldid=605867826.
• Fluorescence_correlation_spectroscopy depiction Fluorescence_correlation_spectroscopy_instrument_diagram.png.
• Fluorescence_correlation_spectroscopy isPrimaryTopicOf Fluorescence_correlation_spectroscopy.