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- Fluorescence_cross-correlation_spectroscopy abstract "Fluorescence cross-correlation spectroscopy (FCCS) was introduced by Eigen and Rigler in 1994 and experimentally realized by Schwille in 1997. It extends the fluorescence correlation spectroscopy (FCS) procedure in that it looks at the correlation between different colors rather than just the same color. In other words, coincident green and red intensity fluctuations correlate if green and red labeled particles are moving together. As a result, FCCS provides a highly sensitive measurement of molecular interactions independent of diffusion rate. This is an important advancement, given that diffusion rate depends only weakly on the size of the molecular complex. FCCS utilizes two species which are independently labeled with two differently colored fluorescent probes. These fluorescent probes are excited and detected by two different laser light sources and detectors usually labeled as “green” and “red”. Typically a confocal microscope is used to provide overlapping green and red focal volumes for excitation. The normalized cross-correlation function is defined for two fluorescent species and which are independent green, G and red, R channels as follows:where differential fluorescent signals at a specific time, and at a delay time, later is correlated with each other. In the absence of spectral bleed-through, the cross-correlation function is zero for non-interacting particles. In contrast to FCS, the cross-correlation function increases with increasing numbers of interacting particles.FCCS is primarily utilized for measurements of bio-molecular interactions both in living cells and in vitro. It can be utilized to measure simple molecular stoichiometry’s and binding constants. It is one of the few techniques that can provide information about protein-protein interactions at a specific time and location within a living cell. In contrast to fluorescence resonance energy transfer, it does not have a distance limit for interactions. As a result it can be utilized to probe large complexes. Nevertheless, it does require that complexes be actively diffusing through the microscope focus on a relatively short time scale (typically seconds).".
- Fluorescence_cross-correlation_spectroscopy thumbnail Fccs_focus_cartoon.svg?width=300.
- Fluorescence_cross-correlation_spectroscopy wikiPageID "12424551".
- Fluorescence_cross-correlation_spectroscopy wikiPageRevisionID "586750221".
- Fluorescence_cross-correlation_spectroscopy confusing "December 2010".
- Fluorescence_cross-correlation_spectroscopy context "December 2010".
- Fluorescence_cross-correlation_spectroscopy hasPhotoCollection Fluorescence_cross-correlation_spectroscopy.
- Fluorescence_cross-correlation_spectroscopy noFootnotes "February 2010".
- Fluorescence_cross-correlation_spectroscopy subject Category:Biochemistry_methods.
- Fluorescence_cross-correlation_spectroscopy subject Category:Fluorescence.
- Fluorescence_cross-correlation_spectroscopy subject Category:Physical_chemistry.
- Fluorescence_cross-correlation_spectroscopy subject Category:Spectroscopy.
- Fluorescence_cross-correlation_spectroscopy type Ability105616246.
- Fluorescence_cross-correlation_spectroscopy type Abstraction100002137.
- Fluorescence_cross-correlation_spectroscopy type BiochemistryMethods.
- Fluorescence_cross-correlation_spectroscopy type Cognition100023271.
- Fluorescence_cross-correlation_spectroscopy type Know-how105616786.
- Fluorescence_cross-correlation_spectroscopy type Method105660268.
- Fluorescence_cross-correlation_spectroscopy type PsychologicalFeature100023100.
- Fluorescence_cross-correlation_spectroscopy comment "Fluorescence cross-correlation spectroscopy (FCCS) was introduced by Eigen and Rigler in 1994 and experimentally realized by Schwille in 1997. It extends the fluorescence correlation spectroscopy (FCS) procedure in that it looks at the correlation between different colors rather than just the same color. In other words, coincident green and red intensity fluctuations correlate if green and red labeled particles are moving together.".
- Fluorescence_cross-correlation_spectroscopy label "Fluorescence cross-correlation spectroscopy".
- Fluorescence_cross-correlation_spectroscopy sameAs m.02w4yxl.
- Fluorescence_cross-correlation_spectroscopy sameAs Q8564081.
- Fluorescence_cross-correlation_spectroscopy sameAs Q8564081.
- Fluorescence_cross-correlation_spectroscopy sameAs Fluorescence_cross-correlation_spectroscopy.
- Fluorescence_cross-correlation_spectroscopy wasDerivedFrom Fluorescence_cross-correlation_spectroscopy?oldid=586750221.
- Fluorescence_cross-correlation_spectroscopy depiction Fccs_focus_cartoon.svg.
- Fluorescence_cross-correlation_spectroscopy isPrimaryTopicOf Fluorescence_cross-correlation_spectroscopy.
