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- Gel_extraction abstract "In molecular biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. After extraction, fragments of interest can be mixed, precipitated, and enzymatically ligated together in several simple steps. This process, usually performed on plasmids, is the basis for rudimentary genetic engineering.After DNA samples are run on an agarose gel, extraction involves four basic steps: identifying the fragments of interest, isolating the corresponding bands, isolating the DNA from those bands, and removing the accompanying salts and stain.To begin, UV light is shone on the gel in order to illuminate all the ethidium bromide-stained DNA. Care must be taken to avoid exposing the DNA to mutagenic radiation for longer than absolutely necessary. The desired band is identified and physically removed with a cover slip or razor blade. The removed slice of gel should contain the desired DNA inside. An alternative method, utilizing SYBR Safe DNA gel stain and blue-light illumination, avoids the DNA damage associated with ethidium bromide and UV light.Several strategies for isolating and cleaning the DNA fragment of interest exist.".
- Gel_extraction wikiPageID "2165654".
- Gel_extraction wikiPageRevisionID "544053520".
- Gel_extraction hasPhotoCollection Gel_extraction.
- Gel_extraction subject Category:Laboratory_techniques.
- Gel_extraction subject Category:Molecular_biology.
- Gel_extraction type Ability105616246.
- Gel_extraction type Abstraction100002137.
- Gel_extraction type Cognition100023271.
- Gel_extraction type Know-how105616786.
- Gel_extraction type LaboratoryTechniques.
- Gel_extraction type Method105660268.
- Gel_extraction type PsychologicalFeature100023100.
- Gel_extraction type Technique105665146.
- Gel_extraction comment "In molecular biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. After extraction, fragments of interest can be mixed, precipitated, and enzymatically ligated together in several simple steps.".
- Gel_extraction label "Gel extraction".
- Gel_extraction sameAs m.06rqqp.
- Gel_extraction sameAs Q5530333.
- Gel_extraction sameAs Q5530333.
- Gel_extraction sameAs Gel_extraction.
- Gel_extraction wasDerivedFrom Gel_extraction?oldid=544053520.
- Gel_extraction isPrimaryTopicOf Gel_extraction.