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- HNCA_experiment abstract "HNCA is a 3D triple-resonance NMR experiment commonly used in the field of protein NMR. The name derives from the experiment's magnetization transfer pathway: The magnetization of the amide proton of an amino acid residue is transferred to the amide nitrogen, and then to the alpha carbons of both the starting residue and the previous residue in the protein's amino acid sequence. In contrast, the complementary HNCOCA experimenttransfers magnetization only to the alpha carbon of the previous residue. The HNCA experiment is used, often in tandem with HNCOCA, to assign alpha carbon resonance signals to specific residues in the protein. This experiment requires a purified sample of protein prepared with 13C and 15N isotopic labelling, at a concentration greater than 0.1 mM, and is thus generally only applied to recombinant proteins.The spectrum produced by this experiment has 3 dimensions: A proton axis, a 15N axis and a 13C axis. For residue i peaks will appear at {HN(i), N(i), Calpha (i)} and {HN(i), N(i), Calpha(i-1)}, while for the complementary HNCOCA experiment peaks appear only at {HN(i), N(i), Calpha(i-1)}. Together, these two experiments reveal the alpha carbon chemical shift for each amino acid residue in a protein, and provide information linking adjacent residues in the protein's sequence.".
- HNCA_experiment thumbnail Hncahncoca.jpg?width=300.
- HNCA_experiment wikiPageID "3663969".
- HNCA_experiment wikiPageRevisionID "591679681".
- HNCA_experiment hasPhotoCollection HNCA_experiment.
- HNCA_experiment subject Category:Biophysics.
- HNCA_experiment subject Category:Nuclear_magnetic_resonance.
- HNCA_experiment subject Category:Protein_methods.
- HNCA_experiment subject Category:Protein_structure.
- HNCA_experiment type Ability105616246.
- HNCA_experiment type Abstraction100002137.
- HNCA_experiment type Cognition100023271.
- HNCA_experiment type Know-how105616786.
- HNCA_experiment type Method105660268.
- HNCA_experiment type ProteinMethods.
- HNCA_experiment type PsychologicalFeature100023100.
- HNCA_experiment comment "HNCA is a 3D triple-resonance NMR experiment commonly used in the field of protein NMR. The name derives from the experiment's magnetization transfer pathway: The magnetization of the amide proton of an amino acid residue is transferred to the amide nitrogen, and then to the alpha carbons of both the starting residue and the previous residue in the protein's amino acid sequence.".
- HNCA_experiment label "HNCA experiment".
- HNCA_experiment sameAs m.09t3cy.
- HNCA_experiment sameAs Q5635300.
- HNCA_experiment sameAs Q5635300.
- HNCA_experiment sameAs HNCA_experiment.
- HNCA_experiment wasDerivedFrom HNCA_experiment?oldid=591679681.
- HNCA_experiment depiction Hncahncoca.jpg.
- HNCA_experiment isPrimaryTopicOf HNCA_experiment.
