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- I-CreI abstract "I-CreI is a homing endonuclease whose gene was first discovered in the chloroplast genome of Chlamydomonas reinhardtii, a species of unicellular green algae. It is named for the facts that: it resides in an Intron; it was isolated from Clamydomonas reinhardtii; it was the first (I) such gene isolated from C. reinhardtii. Its gene resides in a group I intron in the 23S ribosomal RNA gene of the C. reinhardtii chloroplast, and I-CreI is only expressed when its mRNA is spliced from the primary transcript of the 23S gene. I-CreI enzyme, which functions as a homodimer, recognizes a 22-nucleotide sequence of duplex DNA and cleaves one phosphodiester bond on each strand at specific positions. I-CreI is a member of the LAGLIDADG family of homing endonucleases, all of which have a conserved LAGLIDADG amino acid motif that contributes to their associative domains and active sites. When the I-CreI-containing intron encounters a 23S gene lacking the intron, I-CreI enzyme "homes" in on the "intron-minus" allele of 23S and effects its parent intron's insertion into the intron-minus allele. Introns with this behavior are called mobile introns. Because I-CreI provides for its own propagation while conferring no benefit on its host, it is an example of selfish DNA.".
- I-CreI wikiPageID "8142925".
- I-CreI wikiPageRevisionID "596837866".
- I-CreI hasPhotoCollection I-CreI.
- I-CreI subject Category:Biotechnology.
- I-CreI subject Category:Molecular_biology.
- I-CreI subject Category:Restriction_enzymes.
- I-CreI type Abstraction100002137.
- I-CreI type Activator114723079.
- I-CreI type Catalyst114723628.
- I-CreI type Chemical114806838.
- I-CreI type Compound114818238.
- I-CreI type Endonuclease114605415.
- I-CreI type Enzyme114732946.
- I-CreI type Macromolecule114944888.
- I-CreI type Material114580897.
- I-CreI type Matter100020827.
- I-CreI type Molecule114682133.
- I-CreI type Nuclease114963916.
- I-CreI type OrganicCompound114727670.
- I-CreI type Part113809207.
- I-CreI type PhysicalEntity100001930.
- I-CreI type Protein114728724.
- I-CreI type Relation100031921.
- I-CreI type RestrictionEndonuclease115005071.
- I-CreI type RestrictionEnzymes.
- I-CreI type Substance100019613.
- I-CreI type Thing100002452.
- I-CreI type Unit109465459.
- I-CreI comment "I-CreI is a homing endonuclease whose gene was first discovered in the chloroplast genome of Chlamydomonas reinhardtii, a species of unicellular green algae. It is named for the facts that: it resides in an Intron; it was isolated from Clamydomonas reinhardtii; it was the first (I) such gene isolated from C. reinhardtii. Its gene resides in a group I intron in the 23S ribosomal RNA gene of the C.".
- I-CreI label "I-CreI".
- I-CreI sameAs m.026t4wv.
- I-CreI sameAs Q5967611.
- I-CreI sameAs Q5967611.
- I-CreI sameAs I-CreI.
- I-CreI wasDerivedFrom I-CreI?oldid=596837866.
- I-CreI isPrimaryTopicOf I-CreI.