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• In_silico_PCR abstract "In silico PCR refers to computational tools used to calculate theoretical polymerase chain reaction (PCR) results using a given set of primers (probes) to amplify DNA sequences from a sequenced genome or transcriptome.These tools are used to optimize the design of primers for target DNA or cDNA sequences. Primer optimization has two goals: efficiency and selectivity. Efficiency involves taking into account such factors as GC-content, efficiency of binding, complementarity, secondary structure, and annealing and melting point (Tm). Primer selectivity requires that the primer pairs not fortuitously bind to random sites other than the target of interest, nor should the primer pairs bind to conserved regions of a gene family. If the selectivity is poor, a set of primers will amplify multiple products besides the target of interest.The design of appropriate short or long primer pairs is only one goal of PCR product prediction. Other information provided by in silico PCR tools may include determining primer location, orientation, length of each amplicon, simulation of electrophoretic mobility, identification of open reading frames, and links to other web resources. Many software packages are available offering differing balances of feature set, ease of use, efficiency, and cost. Possibly the most widely used would be e-PCR, freely accessible from the National Center for Biotechnology Information (NCBI) website. On the other hand, FastPCR, a commercial application, allows simultaneous testing of a single primer or a set of primers designed for multiplex target sequences. It performs a fast, gapless alignment to test the complementarity of the primers to the target sequences. Probable PCR products can be found for linear and circular templates using standard or inverse PCR as well as for multiplex PCR. VPCR runs a dynamic simulation of multiplex PCR, allowing for an estimate of quantitative competition effects between multiple amplicons in one reaction.A primer may bind to many predicted sequences, but only sequences with no or few mismatches (1 or 2, depending on location and nucleotide) at the 3' end of the primer can be used for polymerase extension. The last 10-12 bases at the 3' end of a primer are sensitive to initiation of polymerase extension and general primer stability on the template binding site. The effect of a single mismatch at these last 10 bases at the 3' end of the primer depends on its position and local structure, reducing the primer binding, selectivity, and PCR efficiency.".
• In_silico_PCR thumbnail In_silico_PCR_example_result_(jPCR_result).png?width=300.
• In_silico_PCR wikiPageExternalLink insilico.ehu.es.
• In_silico_PCR wikiPageExternalLink tools.
• In_silico_PCR wikiPageExternalLink csPCR.
• In_silico_PCR wikiPageExternalLink e-pcr.
• In_silico_PCR wikiPageID "34964561".
• In_silico_PCR wikiPageRevisionID "601485490".
• In_silico_PCR hasPhotoCollection In_silico_PCR.
• In_silico_PCR subject Category:Bioinformatics.
• In_silico_PCR subject Category:Nucleic_acids.
• In_silico_PCR type Abstraction100002137.
• In_silico_PCR type Chemical114806838.
• In_silico_PCR type Compound114818238.
• In_silico_PCR type Macromolecule114944888.
• In_silico_PCR type Material114580897.
• In_silico_PCR type Matter100020827.
• In_silico_PCR type Molecule114682133.
• In_silico_PCR type NucleicAcid114964129.
• In_silico_PCR type NucleicAcids.
• In_silico_PCR type OrganicCompound114727670.
• In_silico_PCR type Part113809207.
• In_silico_PCR type PhysicalEntity100001930.
• In_silico_PCR type Relation100031921.
• In_silico_PCR type Substance100019613.
• In_silico_PCR type Thing100002452.
• In_silico_PCR type Unit109465459.
• In_silico_PCR comment "In silico PCR refers to computational tools used to calculate theoretical polymerase chain reaction (PCR) results using a given set of primers (probes) to amplify DNA sequences from a sequenced genome or transcriptome.These tools are used to optimize the design of primers for target DNA or cDNA sequences. Primer optimization has two goals: efficiency and selectivity.".
• In_silico_PCR label "In silico PCR".
• In_silico_PCR label "PCR in silico".
• In_silico_PCR label "Виртуальная ПЦР".
• In_silico_PCR sameAs PCR_in_silico.
• In_silico_PCR sameAs m.0j43plj.
• In_silico_PCR sameAs Q4112082.
• In_silico_PCR sameAs Q4112082.
• In_silico_PCR sameAs In_silico_PCR.
• In_silico_PCR wasDerivedFrom In_silico_PCR?oldid=601485490.
• In_silico_PCR depiction In_silico_PCR_example_result_(jPCR_result).png.
• In_silico_PCR isPrimaryTopicOf In_silico_PCR.