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- Isocaudomer abstract "Isocaudomers are pairs of restriction enzymes that have slightly different recognition sequences but upon cleavage generate identical termini. For example the enzymes Mbo I and BamH I are isocaudomers:Mbo I N*GATC N N CTAG*NBamH I G*GATC C C CTAG*GN represents any of the four nucleotides. Independently of which nucleotide is present when cleaving with MboI, after cleavage with either enzyme, all termini have the central tetranucleotide - GATC. This allows fragments generated with one enzyme to anneal with fragments generated with the other enzyme. This can be used for elimination of restriction sites from the resulting DNA fragment. For example:Not I GC*GGCC GC CG CCGG*CGBsp120 I G*GGCC C C CCGG*GIn the above example, both enzymes produce tetranucleotides CCGG which can anneal to one another. However, resulting DNA sequence will be: GCGGCCC GCCCGGGwhere the nucleotides shown in italic originate from NotI-cut site, and those in bold from Bsp120I-cut one. Note that the resulting sequence is not recognised by either of the two enzymes.Other examples of isocaudomers include:NcoI/PagI/PscI/NdeIXbaI/NheI".
- Isocaudomer wikiPageID "7172777".
- Isocaudomer wikiPageRevisionID "584456480".
- Isocaudomer hasPhotoCollection Isocaudomer.
- Isocaudomer subject Category:Enzymes.
- Isocaudomer subject Category:Restriction_enzymes.
- Isocaudomer type Abstraction100002137.
- Isocaudomer type Activator114723079.
- Isocaudomer type Catalyst114723628.
- Isocaudomer type Chemical114806838.
- Isocaudomer type Compound114818238.
- Isocaudomer type Endonuclease114605415.
- Isocaudomer type Enzyme114732946.
- Isocaudomer type Enzymes.
- Isocaudomer type Macromolecule114944888.
- Isocaudomer type Material114580897.
- Isocaudomer type Matter100020827.
- Isocaudomer type Molecule114682133.
- Isocaudomer type Nuclease114963916.
- Isocaudomer type OrganicCompound114727670.
- Isocaudomer type Part113809207.
- Isocaudomer type PhysicalEntity100001930.
- Isocaudomer type Protein114728724.
- Isocaudomer type Relation100031921.
- Isocaudomer type RestrictionEndonuclease115005071.
- Isocaudomer type RestrictionEnzymes.
- Isocaudomer type Substance100019613.
- Isocaudomer type Thing100002452.
- Isocaudomer type Unit109465459.
- Isocaudomer comment "Isocaudomers are pairs of restriction enzymes that have slightly different recognition sequences but upon cleavage generate identical termini. For example the enzymes Mbo I and BamH I are isocaudomers:Mbo I N*GATC N N CTAG*NBamH I G*GATC C C CTAG*GN represents any of the four nucleotides. Independently of which nucleotide is present when cleaving with MboI, after cleavage with either enzyme, all termini have the central tetranucleotide - GATC.".
- Isocaudomer label "Isocaudomer".
- Isocaudomer sameAs m.0h7_hy.
- Isocaudomer sameAs Q17000139.
- Isocaudomer sameAs Q17000139.
- Isocaudomer sameAs Isocaudomer.
- Isocaudomer wasDerivedFrom Isocaudomer?oldid=584456480.
- Isocaudomer isPrimaryTopicOf Isocaudomer.