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- Isoelectric_point abstract "The isoelectric point (pI), sometimes abbreviated to IEP, is the pH at which a particular molecule or surface carries no net electrical charge. The net charge on the molecule is affected by pH of its surrounding environment and can become more positively or negatively charged due to the gain or loss, respectively, of protons (H+). Surfaces naturally charge to form a double layer. In the common case when the surface charge-determining ions are H+/OH-, the net surface charge is affected by the pH of the liquid in which the solid is submerged.The pI value can affect the solubility of a molecule at a given pH. Such molecules have minimum solubility in water or salt solutions at the pH that corresponds to their pI and often precipitate out of solution. Biological amphoteric molecules such as proteins contain both acidic and basic functional groups. Amino acids that make up proteins may be positive, negative, neutral, or polar in nature, and together give a protein its overall charge. At a pH below their pI, proteins carry a net positive charge; above their pI they carry a net negative charge. Proteins can, thus, be separated according to their isoelectric point (overall charge) on a polyacrylamide gel using a technique called isoelectric focusing, which uses a pH gradient to separate proteins. Isoelectric focusing is also the first step in 2-D gel polyacrylamide gel electrophoresis.".
- Isoelectric_point thumbnail Glycine_pI.png?width=300.
- Isoelectric_point wikiPageExternalLink isoelectric.ovh.org.
- Isoelectric_point wikiPageExternalLink pi-wrapper.pl.
- Isoelectric_point wikiPageExternalLink Curtipot_.html.
- Isoelectric_point wikiPageID "15056".
- Isoelectric_point wikiPageRevisionID "605673266".
- Isoelectric_point hasPhotoCollection Isoelectric_point.
- Isoelectric_point subject Category:Ions.
- Isoelectric_point subject Category:Molecular_biology.
- Isoelectric_point type Body109224911.
- Isoelectric_point type Ion109315159.
- Isoelectric_point type Ions.
- Isoelectric_point type NaturalObject100019128.
- Isoelectric_point type Object100002684.
- Isoelectric_point type Particle109386422.
- Isoelectric_point type PhysicalEntity100001930.
- Isoelectric_point type Whole100003553.
- Isoelectric_point comment "The isoelectric point (pI), sometimes abbreviated to IEP, is the pH at which a particular molecule or surface carries no net electrical charge. The net charge on the molecule is affected by pH of its surrounding environment and can become more positively or negatively charged due to the gain or loss, respectively, of protons (H+). Surfaces naturally charge to form a double layer.".
- Isoelectric_point label "Iso-elektrisch punt".
- Isoelectric_point label "Isoelectric point".
- Isoelectric_point label "Point isoélectrique".
- Isoelectric_point label "Ponto isoelétrico".
- Isoelectric_point label "Punkt izoelektryczny".
- Isoelectric_point label "Punto isoelettrico".
- Isoelectric_point label "Punto isoeléctrico".
- Isoelectric_point label "Изоэлектрическая точка".
- Isoelectric_point label "نقطة تساوي الكهربائية".
- Isoelectric_point label "等电点".
- Isoelectric_point label "等電点".
- Isoelectric_point sameAs Izoelektrický_bod.
- Isoelectric_point sameAs Ισοηλεκτρικό_σημείο.
- Isoelectric_point sameAs Punto_isoeléctrico.
- Isoelectric_point sameAs Point_isoélectrique.
- Isoelectric_point sameAs Titik_isoelektrik.
- Isoelectric_point sameAs Punto_isoelettrico.
- Isoelectric_point sameAs 等電点.
- Isoelectric_point sameAs 등전점.
- Isoelectric_point sameAs Iso-elektrisch_punt.
- Isoelectric_point sameAs Punkt_izoelektryczny.
- Isoelectric_point sameAs Ponto_isoelétrico.
- Isoelectric_point sameAs m.03wr2.
- Isoelectric_point sameAs Q308708.
- Isoelectric_point sameAs Q308708.
- Isoelectric_point sameAs Isoelectric_point.
- Isoelectric_point wasDerivedFrom Isoelectric_point?oldid=605673266.
- Isoelectric_point depiction Glycine_pI.png.
- Isoelectric_point isPrimaryTopicOf Isoelectric_point.
