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- Job_plot abstract "A Job plot (also known as the method of continuous variation or Job's method; named after P. Job) is used to determine the stoichiometry of a binding event. This method is widely used in analytical chemistry, instrumental analysis, and advanced chemical equilibrium texts and research articles.In solutions where two species are present (i.e. species A and species B), one species (A) may bind to the other species (B). In some cases, more than one A will bind with a single B. One way to determine the amount of A binding to B is by using a Job plot.In this method, the total molar concentration of the two binding partners (e.g. a protein and ligand or a metal and a ligand) are held constant, but their mole fractions are varied. An observable that is proportional to complex formation (such as absorption signal or enzymatic activity) is plotted against the mole fractions of these two components. The maximum (or minimum) on the plot corresponds to the stoichiometry of the two species if sufficiently high concentrations are used. This method is named after P. Job, who introduced this methodology in 1928. An early work of I. Ostromisslensky describes essentially the same approach There are several conditions that must be met in order for Job's method to be applicable:The system must conform to Beer's lawOne complex must predominate under the conditions of the experimentThe Total concentration of the two binding partners must be maintained constantpH and ionic strength must be maintained constant↑ ↑ ↑ ↑".
- Job_plot wikiPageID "13126988".
- Job_plot wikiPageRevisionID "555613527".
- Job_plot hasPhotoCollection Job_plot.
- Job_plot subject Category:Scientific_techniques.
- Job_plot type Ability105616246.
- Job_plot type Abstraction100002137.
- Job_plot type Cognition100023271.
- Job_plot type Know-how105616786.
- Job_plot type Method105660268.
- Job_plot type PsychologicalFeature100023100.
- Job_plot type ScientificTechniques.
- Job_plot type Technique105665146.
- Job_plot comment "A Job plot (also known as the method of continuous variation or Job's method; named after P. Job) is used to determine the stoichiometry of a binding event. This method is widely used in analytical chemistry, instrumental analysis, and advanced chemical equilibrium texts and research articles.In solutions where two species are present (i.e. species A and species B), one species (A) may bind to the other species (B). In some cases, more than one A will bind with a single B.".
- Job_plot label "Job plot".
- Job_plot sameAs m.02z7q8g.
- Job_plot sameAs Q6206742.
- Job_plot sameAs Q6206742.
- Job_plot sameAs Job_plot.
- Job_plot wasDerivedFrom Job_plot?oldid=555613527.
- Job_plot isPrimaryTopicOf Job_plot.