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- Light_sheet_fluorescence_microscopy abstract "Light sheet fluorescence microscopy (LSFM) is a fluorescence microscopy technique with an intermediate optical resolution, but good sectioning capabilities. In contrast to epi-fluorescence microscopy only a thin slice (usually a few hundred nanometers to a few micrometers) of the sample is illuminated perpendicularly to the direction of observation. For illumination a laser lightsheet is used, i.e. a laserbeam which is focused only in one direction (e.g. using a cylindrical lens). A second method uses a circular beam scanned in one direction to create the lightsheet. As only the actually observed section is illuminated, this method reduces the photodamage and stress induced on a living sample. Also the good sectioning capability reduces the background signal and thus creates images with higher contrast, comparable to confocal microscopy.This method is used in cellular biology and for microscopy of whole living creatures, such as embryos. It is now often used for the longtime observation of embryonal development in different model organisms.Starting in 1994, LSFM was developed as orthogonal plane fluorescence optical sectioning microscopy or tomography (OPFOS) mainly for large samples and later as the selective/single plane illumination microscopy (SPIM) also with sub-cellular resolution. This introduced an illumination scheme into fluorescence microscopy, which has already been used successfully for dark field microscopy under the name ultramicroscopy.".
- Light_sheet_fluorescence_microscopy thumbnail Spim_prinziple_en.svg?width=300.
- Light_sheet_fluorescence_microscopy wikiPageExternalLink dev.022426.
- Light_sheet_fluorescence_microscopy wikiPageExternalLink 0022155410394857.
- Light_sheet_fluorescence_microscopy wikiPageExternalLink 206238.
- Light_sheet_fluorescence_microscopy wikiPageExternalLink watch?v=MefTPoeVQ3w&feature=share&list=PL7FC088E07700172B.
- Light_sheet_fluorescence_microscopy wikiPageID "37430358".
- Light_sheet_fluorescence_microscopy wikiPageRevisionID "604607271".
- Light_sheet_fluorescence_microscopy hasPhotoCollection Light_sheet_fluorescence_microscopy.
- Light_sheet_fluorescence_microscopy subject Category:Cell_imaging.
- Light_sheet_fluorescence_microscopy subject Category:Fluorescence.
- Light_sheet_fluorescence_microscopy subject Category:Laboratory_equipment.
- Light_sheet_fluorescence_microscopy subject Category:Microscopes.
- Light_sheet_fluorescence_microscopy subject Category:Microscopy.
- Light_sheet_fluorescence_microscopy comment "Light sheet fluorescence microscopy (LSFM) is a fluorescence microscopy technique with an intermediate optical resolution, but good sectioning capabilities. In contrast to epi-fluorescence microscopy only a thin slice (usually a few hundred nanometers to a few micrometers) of the sample is illuminated perpendicularly to the direction of observation. For illumination a laser lightsheet is used, i.e. a laserbeam which is focused only in one direction (e.g. using a cylindrical lens).".
- Light_sheet_fluorescence_microscopy label "Lichtscheibenmikroskopie".
- Light_sheet_fluorescence_microscopy label "Light sheet fluorescence microscopy".
- Light_sheet_fluorescence_microscopy label "Selective Plane Illumination Microscopy".
- Light_sheet_fluorescence_microscopy sameAs Lichtscheibenmikroskopie.
- Light_sheet_fluorescence_microscopy sameAs Selective_Plane_Illumination_Microscopy.
- Light_sheet_fluorescence_microscopy sameAs m.0nb7btl.
- Light_sheet_fluorescence_microscopy sameAs Q1823502.
- Light_sheet_fluorescence_microscopy sameAs Q1823502.
- Light_sheet_fluorescence_microscopy wasDerivedFrom Light_sheet_fluorescence_microscopy?oldid=604607271.
- Light_sheet_fluorescence_microscopy depiction Spim_prinziple_en.svg.
- Light_sheet_fluorescence_microscopy isPrimaryTopicOf Light_sheet_fluorescence_microscopy.
