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• Multiplex_ligation-dependent_probe_amplification abstract "Multiplex ligation-dependent probe amplification (MLPA)[1] is a variation of the multiplex polymerase chain reaction that permits multiple targets to be amplified with only a single primer pair. Each probe consists of two oligonucleotides which recognise adjacent target sites on the DNA. One probe oligonucleotide contains the sequence recognised by the forward primer, the other the sequence recognised by the reverse primer. Only when both probe oligonucleotides are hybridised to their respective targets, can they be ligated into a complete probe. The advantage of splitting the probe into two parts is that only the ligated oligonucleotides, but not the unbound probe oligonucleotides, are amplified. If the probes were not split in this way, the primer sequences at either end would cause the probes to be amplified regardless of their hybridization to the template DNA, and the amplification product would not be dependent on the number of target sites present in the sample DNA. Each complete probe has a unique length, so that its resulting amplicons can be separated and identified by (capillary) electrophoresis. This avoids the resolution limitations of multiplex PCR. Since the forward primer used for probe amplification is fluorescently labeled, each amplicon generates a fluorescent peak which can be detected by a capillary sequencer. Comparing the peak pattern obtained on a given sample with that obtained on various reference samples, the relative quantity of each amplicon can be determined. This ratio is a measure for the ratio in which the target sequence is present in the sample DNA.File:MLPA in GeneMarker.jpgVarious techniques including DGGE (Denaturing Gradient Gel Electrophoresis), DHPLC (Denaturing High Performance Liquid Chromatography), and SSCA (Single Strand Conformation Analysis) effectively identify SNPs and small insertions and deletions. MLPA, however, is one of the only accurate, time-efficient techniques to detect genomic deletions and insertions (one or more entire exons), which are frequent causes of cancers such as hereditary non-polyposis colorectal cancer (HNPCC), breast, and ovarian cancer. MLPA can successfully and easily determine the relative copy number of all exons within a gene simultaneously with high sensitivity.".
• Multiplex_ligation-dependent_probe_amplification thumbnail MLPA_in_GeneMarker.jpg?width=300.
• Multiplex_ligation-dependent_probe_amplification wikiPageExternalLink multiplex-ligation-dependent-probe-amplification-mlpa-analysis.
• Multiplex_ligation-dependent_probe_amplification wikiPageExternalLink www.appliedbiosystems.com.
• Multiplex_ligation-dependent_probe_amplification wikiPageExternalLink Conventional_MLPA.htm.
• Multiplex_ligation-dependent_probe_amplification wikiPageExternalLink REX-MLPA.htm.
• Multiplex_ligation-dependent_probe_amplification wikiPageExternalLink www.mlpa.com.
• Multiplex_ligation-dependent_probe_amplification wikiPageExternalLink mlpa.htm.
• Multiplex_ligation-dependent_probe_amplification wikiPageExternalLink mlpapubs.html.
• Multiplex_ligation-dependent_probe_amplification wikiPageExternalLink www.softgenetics.com.
• Multiplex_ligation-dependent_probe_amplification wikiPageID "9346573".
• Multiplex_ligation-dependent_probe_amplification wikiPageRevisionID "576254156".
• Multiplex_ligation-dependent_probe_amplification hasPhotoCollection Multiplex_ligation-dependent_probe_amplification.
• Multiplex_ligation-dependent_probe_amplification subject Category:Amplifiers.
• Multiplex_ligation-dependent_probe_amplification subject Category:Gene_tests.
• Multiplex_ligation-dependent_probe_amplification subject Category:Laboratory_techniques.
• Multiplex_ligation-dependent_probe_amplification subject Category:Molecular_biology.
• Multiplex_ligation-dependent_probe_amplification subject Category:Polymerase_chain_reaction.
• Multiplex_ligation-dependent_probe_amplification type Ability105616246.
• Multiplex_ligation-dependent_probe_amplification type Abstraction100002137.
• Multiplex_ligation-dependent_probe_amplification type Cognition100023271.
• Multiplex_ligation-dependent_probe_amplification type Experiment105798043.
• Multiplex_ligation-dependent_probe_amplification type GeneTests.
• Multiplex_ligation-dependent_probe_amplification type HigherCognitiveProcess105770664.
• Multiplex_ligation-dependent_probe_amplification type Inquiry105797597.
• Multiplex_ligation-dependent_probe_amplification type Know-how105616786.
• Multiplex_ligation-dependent_probe_amplification type LaboratoryTechniques.
• Multiplex_ligation-dependent_probe_amplification type Method105660268.
• Multiplex_ligation-dependent_probe_amplification type ProblemSolving105796750.
• Multiplex_ligation-dependent_probe_amplification type Process105701363.
• Multiplex_ligation-dependent_probe_amplification type PsychologicalFeature100023100.
• Multiplex_ligation-dependent_probe_amplification type Technique105665146.
• Multiplex_ligation-dependent_probe_amplification type Thinking105770926.
• Multiplex_ligation-dependent_probe_amplification type Trial105799212.
• Multiplex_ligation-dependent_probe_amplification comment "Multiplex ligation-dependent probe amplification (MLPA)[1] is a variation of the multiplex polymerase chain reaction that permits multiple targets to be amplified with only a single primer pair. Each probe consists of two oligonucleotides which recognise adjacent target sites on the DNA. One probe oligonucleotide contains the sequence recognised by the forward primer, the other the sequence recognised by the reverse primer.".
• Multiplex_ligation-dependent_probe_amplification label "MLPA".
• Multiplex_ligation-dependent_probe_amplification label "Multiplex ligation-dependent probe amplification".
• Multiplex_ligation-dependent_probe_amplification label "Multiplex ligation-dependent probe amplification".
• Multiplex_ligation-dependent_probe_amplification sameAs MLPA.
• Multiplex_ligation-dependent_probe_amplification sameAs Multiplex_ligation-dependent_probe_amplification.
• Multiplex_ligation-dependent_probe_amplification sameAs MLPA.
• Multiplex_ligation-dependent_probe_amplification sameAs m.0285d49.
• Multiplex_ligation-dependent_probe_amplification sameAs Q602889.
• Multiplex_ligation-dependent_probe_amplification sameAs Q602889.
• Multiplex_ligation-dependent_probe_amplification sameAs Multiplex_ligation-dependent_probe_amplification.
• Multiplex_ligation-dependent_probe_amplification wasDerivedFrom Multiplex_ligation-dependent_probe_amplification?oldid=576254156.
• Multiplex_ligation-dependent_probe_amplification depiction MLPA_in_GeneMarker.jpg.
• Multiplex_ligation-dependent_probe_amplification isPrimaryTopicOf Multiplex_ligation-dependent_probe_amplification.