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- Negative_stain abstract "Negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrasts with 'positive staining', in which the actual specimen is stained.For bright field microscopy, negative staining is typically performed using a black ink fluid such as nigrosin. The specimen, such as a wet bacterial culture spread on a glass slide, is mixed with the negative stain and allowed to dry. When viewed with the microscope the bacterial cells, and perhaps their spores, appear light against the dark surrounding background. An alternative method has been developed using an ordinary waterproof marking pen to deliver the negative stain.In the case of transmission electron microscopy, opaqueness to electrons is related to the atomic number, i.e., the number of protons. Some suitable negative stains include ammonium molybdate, uranyl acetate, uranyl formate, phosphotungstic acid, osmium tetroxide, osmium ferricyanide and auroglucothionate. These have been chosen because they scatter electrons well and also adsorb to biological matter well. The structures which can be negatively stained are much smaller than those studied with the light microscope. Here, the method is used to view viruses, bacteria, bacterial flagella, biological membrane structures and proteins or protein aggregates, which all have a low electron-scattering power. Some stains, such as osmium tetroxide and osmium ferricyanide, are very chemically active. As strong oxidants, they cross-links lipids mainly by reacting with unsaturated carbon-carbon bonds, and thereby both fix biological membranes in place in tissue samples and simultaneously stain them.The choice of negative stain in electron microscopy can be very important. A study of plant viruses from ngatively stained leaf dips of a diseased plant showed only spherical viruses with one stain and only rod-shaped viruses with another. The verified conclusion was that this plant suffered from a mixed infection by two separate viruses. Negative staining at both light microscope and electron microscope level should never be performed with infectious organisms unless stringent safety precautions are followed. Negative staining is a very mild preparation method and does not reduce the possibility of operator infection.".
- Negative_stain thumbnail OsStaining.jpg?width=300.
- Negative_stain wikiPageID "4094117".
- Negative_stain wikiPageRevisionID "606630777".
- Negative_stain by "no".
- Negative_stain hasPhotoCollection Negative_stain.
- Negative_stain label "Negative staining".
- Negative_stain lcheading "Negative staining".
- Negative_stain onlinebooks "no".
- Negative_stain subject Category:Electron_microscopy.
- Negative_stain subject Category:Electron_microscopy_stains.
- Negative_stain subject Category:Microscopy.
- Negative_stain subject Category:Staining.
- Negative_stain type Abstraction100002137.
- Negative_stain type Appearance104673965.
- Negative_stain type Attribute100024264.
- Negative_stain type ElectronMicroscopyStains.
- Negative_stain type Quality104723816.
- Negative_stain type Stain104695176.
- Negative_stain comment "Negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrasts with 'positive staining', in which the actual specimen is stained.For bright field microscopy, negative staining is typically performed using a black ink fluid such as nigrosin.".
- Negative_stain label "Coloração negativa".
- Negative_stain label "Negative stain".
- Negative_stain label "Tinción negativa".
- Negative_stain label "تلوين سلبي".
- Negative_stain label "负染色法".
- Negative_stain sameAs Tinción_negativa.
- Negative_stain sameAs Coloração_negativa.
- Negative_stain sameAs m.0bhnwf.
- Negative_stain sameAs Q3772564.
- Negative_stain sameAs Q3772564.
- Negative_stain sameAs Negative_stain.
- Negative_stain wasDerivedFrom Negative_stain?oldid=606630777.
- Negative_stain depiction OsStaining.jpg.
- Negative_stain isPrimaryTopicOf Negative_stain.
