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- Oligonucleotide_synthesis abstract "Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence. Whereas enzymes synthesize DNA and RNA in a 5' to 3' direction, chemical oligonucleotide synthesis is carried out in the opposite, 3' to 5' direction. Currently, the process is implemented as solid-phase synthesis using phosphoramidite method and phosphoramidite building blocks derived from protected 2'-deoxynucleosides (dA, dC, dG, and T), ribonucleosides (A, C, G, and U), or chemically modified nucleosides, e.g. LNA, BNA.To obtain the desired oligonucleotide, the building blocks are sequentially coupled to the growing oligonucleotide chain in the order required by the sequence of the product (see Synthetic cycle below). The process has been fully automated since the late 1970s. Upon the completion of the chain assembly, the product is released from the solid phase to solution, deprotected, and collected. The occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of the oligonucleotide being synthesized. Products are often isolated by high-performance liquid chromatography (HPLC) to obtain the desired oligonucleotides in high purity. Typically, synthetic oligonucleotides are single-stranded DNA or RNA molecules around 15–25 bases in length.Oligonucleotides find a variety of applications in molecular biology and medicine. They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes.".
- Oligonucleotide_synthesis thumbnail Todd_synthesis.png?width=300.
- Oligonucleotide_synthesis wikiPageExternalLink OligoDesigner.aspx.
- Oligonucleotide_synthesis wikiPageExternalLink chitem_print.aspx?aid=2894&chid=0.
- Oligonucleotide_synthesis wikiPageID "10663351".
- Oligonucleotide_synthesis wikiPageRevisionID "605863433".
- Oligonucleotide_synthesis hasPhotoCollection Oligonucleotide_synthesis.
- Oligonucleotide_synthesis subject Category:Laboratory_techniques.
- Oligonucleotide_synthesis subject Category:Molecular_biology.
- Oligonucleotide_synthesis type Ability105616246.
- Oligonucleotide_synthesis type Abstraction100002137.
- Oligonucleotide_synthesis type Cognition100023271.
- Oligonucleotide_synthesis type Know-how105616786.
- Oligonucleotide_synthesis type LaboratoryTechniques.
- Oligonucleotide_synthesis type Method105660268.
- Oligonucleotide_synthesis type PsychologicalFeature100023100.
- Oligonucleotide_synthesis type Technique105665146.
- Oligonucleotide_synthesis comment "Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence. Whereas enzymes synthesize DNA and RNA in a 5' to 3' direction, chemical oligonucleotide synthesis is carried out in the opposite, 3' to 5' direction.".
- Oligonucleotide_synthesis label "Chemiczna synteza oligonukleotydów".
- Oligonucleotide_synthesis label "Oligonucleotide synthesis".
- Oligonucleotide_synthesis label "Synthèse d'oligonucléotide".
- Oligonucleotide_synthesis label "Síntesis de oligonucleótidos".
- Oligonucleotide_synthesis label "Синтез олигонуклеотидов".
- Oligonucleotide_synthesis sameAs Síntesis_de_oligonucleótidos.
- Oligonucleotide_synthesis sameAs Synthèse_d'oligonucléotide.
- Oligonucleotide_synthesis sameAs Chemiczna_synteza_oligonukleotydów.
- Oligonucleotide_synthesis sameAs m.02qlmgw.
- Oligonucleotide_synthesis sameAs Q2620256.
- Oligonucleotide_synthesis sameAs Q2620256.
- Oligonucleotide_synthesis sameAs Oligonucleotide_synthesis.
- Oligonucleotide_synthesis wasDerivedFrom Oligonucleotide_synthesis?oldid=605863433.
- Oligonucleotide_synthesis depiction Todd_synthesis.png.
- Oligonucleotide_synthesis isPrimaryTopicOf Oligonucleotide_synthesis.
