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- PAR-CLIP abstract "PAR-CLIP (Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) is a biochemical method used for identifying the binding sites of cellular RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs). The method relies on the incorporation of photoreactive ribonucleoside analogs, such as 4-thiouridine (4-SU) and 6-thioguanosine (6-SG) into nascent RNA transcripts by living cells. Irradiation of the cells by UV light of 365 nm induces efficient crosslinking of photoreactive nucleoside-labeled cellular RNAs to interacting RBPs. Immunoprecipitation of the RBP of interest is followed by isolation of the crosslinked and coimmunoprecipitated RNA. The isolated RNA is converted into a cDNA library and deep sequenced using next-generation sequencing technology.Recently, PAR-CLIP have been applied to determine the transcriptome-wide binding sites of several known RBPs and microRNA-containing ribonucleoprotein complexes at high resolution.".
- PAR-CLIP wikiPageExternalLink dorina.mdc-berlin.de.
- PAR-CLIP wikiPageExternalLink mirtarclip.mbc.nctu.edu.tw.
- PAR-CLIP wikiPageExternalLink software.
- PAR-CLIP wikiPageExternalLink starbase.sysu.edu.cn.
- PAR-CLIP wikiPageExternalLink www.targetscan.org.
- PAR-CLIP wikiPageID "29648821".
- PAR-CLIP wikiPageRevisionID "604680436".
- PAR-CLIP hasPhotoCollection PAR-CLIP.
- PAR-CLIP subject Category:Protein_methods.
- PAR-CLIP subject Category:RNA.
- PAR-CLIP type Ability105616246.
- PAR-CLIP type Abstraction100002137.
- PAR-CLIP type Cognition100023271.
- PAR-CLIP type Know-how105616786.
- PAR-CLIP type Method105660268.
- PAR-CLIP type ProteinMethods.
- PAR-CLIP type PsychologicalFeature100023100.
- PAR-CLIP comment "PAR-CLIP (Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) is a biochemical method used for identifying the binding sites of cellular RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs). The method relies on the incorporation of photoreactive ribonucleoside analogs, such as 4-thiouridine (4-SU) and 6-thioguanosine (6-SG) into nascent RNA transcripts by living cells.".
- PAR-CLIP label "PAR-CLIP".
- PAR-CLIP label "PAR-CLIP".
- PAR-CLIP sameAs PAR-CLIP.
- PAR-CLIP sameAs m.0fpk10c.
- PAR-CLIP sameAs Q7118193.
- PAR-CLIP sameAs Q7118193.
- PAR-CLIP sameAs PAR-CLIP.
- PAR-CLIP wasDerivedFrom PAR-CLIP?oldid=604680436.
- PAR-CLIP isPrimaryTopicOf PAR-CLIP.