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- PGLO abstract "The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably for the green fluorescent protein (GFP) and the ampicillin resistance gene. GFP was isolated from the jelly fish Aequorea victoria. Because it shares a bidirectional promoter with a gene for metabolizing arabinose, the GFP gene is expressed in the presence of arabinose, which makes the transgenic organism fluoresce under UV light. GFP can be induced in bacteria containing the pGLO plasmid by growing them on +arabinose plates. pGLO is made up of three genes that are joined together using recombinant DNA technology. They are as follows:-Bla, which codes for the enzyme beta-lactamase giving the transformed bacteria resistance to the beta-lactam family of antibiotics (such as of the penicillin family)-araC, a promoter region that regulates the expression of GFP (specifically, the GFP gene will be expressed only in the presence of arabinose)-GFP, the green fluorescent protein, which gives a green glow if cells produce this type of proteinLike most other circular plasmids, the pGLO plasmid contains an origin (ori), which is a region of the plasmid where replication will originate.The pGLO plasmid was made famous by researchers in France who used it to produce a green fluorescent rabbit named Alba.Other features on pGLO, like most other plasmids, include: a selectable marker, Ori (origin of replication), and an MCS (multiple cloning site) located at the end of the GFP gene. The plasmid is 5371 base pairs long. In supercoiled form, it runs on an agarose gel in the 4200-4500 range.".
- PGLO thumbnail PLGO_under_ambient_light.jpg?width=300.
- PGLO wikiPageID "1877429".
- PGLO wikiPageRevisionID "599935683".
- PGLO align "right".
- PGLO footer "Left: pGLO under ambient light".
- PGLO footer "Right: pGLO visualized under ultraviolet light".
- PGLO hasPhotoCollection PGLO.
- PGLO image "PGlo-UltraViolet.jpg".
- PGLO image "PLGO_under_ambient_light.jpg".
- PGLO width "180".
- PGLO width "186".
- PGLO subject Category:Fluorescence_techniques.
- PGLO subject Category:Genetically_modified_organisms.
- PGLO subject Category:Plasmids.
- PGLO type Body109224911.
- PGLO type GeneticallyModifiedOrganisms.
- PGLO type InclusionBody109310806.
- PGLO type LivingThing100004258.
- PGLO type NaturalObject100019128.
- PGLO type Object100002684.
- PGLO type Organism100004475.
- PGLO type PhysicalEntity100001930.
- PGLO type Plasmid109395266.
- PGLO type Plasmids.
- PGLO type Whole100003553.
- PGLO comment "The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably for the green fluorescent protein (GFP) and the ampicillin resistance gene. GFP was isolated from the jelly fish Aequorea victoria.".
- PGLO label "PGLO".
- PGLO label "PGLO".
- PGLO label "PGLO".
- PGLO label "PGLO".
- PGLO sameAs PGLO.
- PGLO sameAs Plasmid_pGLO.
- PGLO sameAs PGLO.
- PGLO sameAs PGLO.
- PGLO sameAs m.06326y.
- PGLO sameAs Q726697.
- PGLO sameAs Q726697.
- PGLO sameAs PGLO.
- PGLO wasDerivedFrom PGLO?oldid=599935683.
- PGLO depiction PLGO_under_ambient_light.jpg.
- PGLO isPrimaryTopicOf PGLO.
