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- PSF_Lab abstract "PSF Lab is a software program that allows the calculation of the illumination point spread function (PSF) of a confocal microscope under various imaging conditions. The calculation of the electric field vectors is based on a rigorous, vectorial model that takes polarization effects in the near-focus region and high numerical aperture microscope objectives into account.The polarization of the input beam (assumed to be collimated and monochromatic) can be chosen freely (linear, circular, or elliptic). Furthermore, a constant or Gaussian shaped input beam intensity profile can be assumed. On its way from the objective to the focus, the illumination light passes through up to three stratified optical layers, which allows the simulation of an immersion oil/air (layer 1) objective that focusses light through a glass cover slip (layer 2) into the sample medium (layer 3). Each layer is characterized by its (constant) refractive index and thickness. PSF Lab can also simulate microscope objectives that are corrected for certain refractive indices and cover slip thicknesses (design parameters). Thus, any deviations from the ideal imaging conditions for which the objective was designed for are properly taken into account.The following optical parameters can be selected: Input beam Wavelength Gaussian profile filling parameter (0 = constant profile) Polarization (linear, circular, elliptic) Outputs Individual field components Squared field components Intensity Microscope objective Numerical aperture Optical media Refractive index (design and actual) Thickness (design and actual) Depth (focus position within medium 3)The program calculates only 2D section of the PSF, but several calculations can be stacked (with a third party program) to obtain the full 3D PSF. Calculations are organized in "sets", each with its own set of parameters. Loops can be set up such that PSF Lab calculates one or several sets, increasing the resolution of the calculated images in each new iteration. The resulting image is displayed in PSF Lab in linear or logarithmic color scale with user-selectable color map, and the intensity, individual field components, or squared field component distributions can be exported into various formats (data formats: .mat, .h5 (HDF5), .txt (ASCII); image formats: .fig, .ai, .bmp, .emf, .eps, .jpg, .pcx, .pdf, .png, .tif).".
- PSF_Lab frequentlyUpdated "yes".
- PSF_Lab genre List_of_computer_simulation_software.
- PSF_Lab latestReleaseDate "2010-09-24".
- PSF_Lab latestReleaseVersion "3.0".
- PSF_Lab operatingSystem Mac_OS.
- PSF_Lab operatingSystem Microsoft_Windows.
- PSF_Lab thumbnail Wiki_PSF_Lab_3.0_screenshot_1.PNG?width=300.
- PSF_Lab wikiPageExternalLink deconintro.html.
- PSF_Lab wikiPageExternalLink onemolecule.chem.uwm.edu.
- PSF_Lab wikiPageExternalLink software.
- PSF_Lab wikiPageExternalLink www.uwmresearchfoundation.org.
- PSF_Lab wikiPageID "26592028".
- PSF_Lab wikiPageRevisionID "560966064".
- PSF_Lab caption "PSF Lab on Windows 7".
- PSF_Lab developer "Michael J. Nasse, Jörg C. Woehl".
- PSF_Lab frequentlyUpdated "yes".
- PSF_Lab genre List_of_computer_simulation_software.
- PSF_Lab hasPhotoCollection PSF_Lab.
- PSF_Lab latestReleaseDate "2010-09-24".
- PSF_Lab latestReleaseVersion "3".
- PSF_Lab license "Copyright 2010, UWM Research Foundation".
- PSF_Lab name "PSF Lab".
- PSF_Lab operatingSystem Mac_OS.
- PSF_Lab operatingSystem Microsoft_Windows.
- PSF_Lab screenshot "200".
- PSF_Lab website software.
- PSF_Lab subject Category:Optical_software.
- PSF_Lab subject Category:Physics_software.
- PSF_Lab type Abstraction100002137.
- PSF_Lab type Code106355894.
- PSF_Lab type CodingSystem106353757.
- PSF_Lab type Communication100033020.
- PSF_Lab type Software106566077.
- PSF_Lab type Writing106359877.
- PSF_Lab type WrittenCommunication106349220.
- PSF_Lab type Software.
- PSF_Lab type Work.
- PSF_Lab type CreativeWork.
- PSF_Lab type InformationEntity.
- PSF_Lab comment "PSF Lab is a software program that allows the calculation of the illumination point spread function (PSF) of a confocal microscope under various imaging conditions.".
- PSF_Lab label "PSF Lab".
- PSF_Lab sameAs m.0bmj2c3.
- PSF_Lab sameAs Q7120632.
- PSF_Lab sameAs Q7120632.
- PSF_Lab sameAs PSF_Lab.
- PSF_Lab wasDerivedFrom PSF_Lab?oldid=560966064.
- PSF_Lab depiction Wiki_PSF_Lab_3.0_screenshot_1.PNG.
- PSF_Lab homepage software.
- PSF_Lab isPrimaryTopicOf PSF_Lab.
- PSF_Lab name "PSF Lab".
%20of%20a%20confocal%20microscope%20under%20various%20imaging%20conditions.%20The%20calculation%20of%20the%20electric%20field%20vectors%20is%20based%20on%20a%20rigorous%2C%20vectorial%20model%20that%20takes%20polarization%20effects%20in%20the%20near-focus%20region%20and%20high%20numerical%20aperture%20microscope%20objectives%20into%20account.The%20polarization%20of%20the%20input%20beam%20(assumed%20to%20be%20collimated%20and%20monochromatic)%20can%20be%20chosen%20freely%20(linear%2C%20circular%2C%20or%20elliptic).%20Furthermore%2C%20a%20constant%20or%20Gaussian%20shaped%20input%20beam%20intensity%20profile%20can%20be%20assumed.%20On%20its%20way%20from%20the%20objective%20to%20the%20focus%2C%20the%20illumination%20light%20passes%20through%20up%20to%20three%20stratified%20optical%20layers%2C%20which%20allows%20the%20simulation%20of%20an%20immersion%20oil%2Fair%20(layer%201)%20objective%20that%20focusses%20light%20through%20a%20glass%20cover%20slip%20(layer%202)%20into%20the%20sample%20medium%20(layer%203).%20Each%20layer%20is%20characterized%20by%20its%20(constant)%20refractive%20index%20and%20thickness.%20PSF%20Lab%20can%20also%20simulate%20microscope%20objectives%20that%20are%20corrected%20for%20certain%20refractive%20indices%20and%20cover%20slip%20thicknesses%20(design%20parameters).%20Thus%2C%20any%20deviations%20from%20the%20ideal%20imaging%20conditions%20for%20which%20the%20objective%20was%20designed%20for%20are%20properly%20taken%20into%20account.The%20following%20optical%20parameters%20can%20be%20selected%3A%20Input%20beam%20Wavelength%20Gaussian%20profile%20filling%20parameter%20(0%20%3D%20constant%20profile)%20Polarization%20(linear%2C%20circular%2C%20elliptic)%20Outputs%20Individual%20field%20components%20Squared%20field%20components%20Intensity%20Microscope%20objective%20Numerical%20aperture%20Optical%20media%20Refractive%20index%20(design%20and%20actual)%20Thickness%20(design%20and%20actual)%20Depth%20(focus%20position%20within%20medium%203)The%20program%20calculates%20only%202D%20section%20of%20the%20PSF%2C%20but%20several%20calculations%20can%20be%20stacked%20(with%20a%20third%20party%20program)%20to%20obtain%20the%20full%203D%20PSF.%20Calculations%20are%20organized%20in%20%22sets%22%2C%20each%20with%20its%20own%20set%20of%20parameters.%20Loops%20can%20be%20set%20up%20such%20that%20PSF%20Lab%20calculates%20one%20or%20several%20sets%2C%20increasing%20the%20resolution%20of%20the%20calculated%20images%20in%20each%20new%20iteration.%20The%20resulting%20image%20is%20displayed%20in%20PSF%20Lab%20in%20linear%20or%20logarithmic%20color%20scale%20with%20user-selectable%20color%20map%2C%20and%20the%20intensity%2C%20individual%20field%20components%2C%20or%20squared%20field%20component%20distributions%20can%20be%20exported%20into%20various%20formats%20(data%20formats%3A%20.mat%2C%20.h5%20(HDF5)%2C%20.txt%20(ASCII)%3B%20image%20formats%3A%20.fig%2C%20.ai%2C%20.bmp%2C%20.emf%2C%20.eps%2C%20.jpg%2C%20.pcx%2C%20.pdf%2C%20.png%2C%20.tif).%22%40en){kind=link}
