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- Paired-end_tag abstract "Paired-end tags (PET) (sometimes "Paired-End diTags", or simply "ditags") are the short sequences at the 5’ and 3’ ends of a DNA fragment which are unique enough that they (theoretically) exist together only once in a genome, therefore making the sequence of the DNA in between them available upon search (if full-genome sequence data is available) or upon further sequencing (since tag sites are unique enough to serve as primer annealing sites). Paired-end tags (PET) exist in PET libraries with the intervening DNA absent, that is, a PET "represents" a larger fragment of genomic or cDNA by consisting of a short 5' linker sequence, a short 5' sequence tag, a short 3' sequence tag, and a short 3' linker sequence. It was shown conceptually that 13 bp is sufficient to map tags uniquely. However, longer sequences are more practical for mapping reads uniquely. The endonucleases (discussed below) used to produce PETs give longer tags (18/20 bp and 25/27 bp) but sequences of 50–100 base pairs would be optimal for both mapping and cost efficiency. After extracting the PETs from many DNA fragments, they are linked (concatenated) together for efficient sequencing. On average, 20–30 tags could be sequenced with the Sanger method, which has a longer read length. Since the tag sequences are short, individual PETs are well suited for next-generation sequencing that has short read lengths and higher throughput. The main advantages of PET sequencing are its reduced cost by sequencing only short fragments, detection of structural variants in the genome, and increased specificity when aligning back to the genome compared to single tags, which involves only one end of the DNA fragment.".
- Paired-end_tag thumbnail PETworkflow.png?width=300.
- Paired-end_tag wikiPageID "26224857".
- Paired-end_tag wikiPageRevisionID "550465439".
- Paired-end_tag hasPhotoCollection Paired-end_tag.
- Paired-end_tag subject Category:DNA_sequencing.
- Paired-end_tag subject Category:Laboratory_techniques.
- Paired-end_tag subject Category:Molecular_biology.
- Paired-end_tag subject Category:Molecular_biology_techniques.
- Paired-end_tag type Ability105616246.
- Paired-end_tag type Abstraction100002137.
- Paired-end_tag type Cognition100023271.
- Paired-end_tag type Know-how105616786.
- Paired-end_tag type LaboratoryTechniques.
- Paired-end_tag type Method105660268.
- Paired-end_tag type MolecularBiologyTechniques.
- Paired-end_tag type PsychologicalFeature100023100.
- Paired-end_tag type Technique105665146.
- Paired-end_tag comment "Paired-end tags (PET) (sometimes "Paired-End diTags", or simply "ditags") are the short sequences at the 5’ and 3’ ends of a DNA fragment which are unique enough that they (theoretically) exist together only once in a genome, therefore making the sequence of the DNA in between them available upon search (if full-genome sequence data is available) or upon further sequencing (since tag sites are unique enough to serve as primer annealing sites).".
- Paired-end_tag label "Paired-end tag".
- Paired-end_tag label "Секвенирование спаренных концов".
- Paired-end_tag sameAs m.0b775j1.
- Paired-end_tag sameAs Q7125131.
- Paired-end_tag sameAs Q7125131.
- Paired-end_tag sameAs Paired-end_tag.
- Paired-end_tag wasDerivedFrom Paired-end_tag?oldid=550465439.
- Paired-end_tag depiction PETworkflow.png.
- Paired-end_tag isPrimaryTopicOf Paired-end_tag.
