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- Ponceau_S abstract "Ponceau S, Acid Red 112, or C.I. 27195 is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting), as well as on cellulose acetate membranes. A Ponceau S stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on Western blots for blot-sequencing. It is also easily reversed with water washes, facilitating subsequent immunological detection. The stain can be completely removed from the protein bands by continued washing. Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid.".
- Ponceau_S thumbnail Ponceau_S.svg?width=300.
- Ponceau_S wikiPageID "3208346".
- Ponceau_S wikiPageRevisionID "598998209".
- Ponceau_S hasPhotoCollection Ponceau_S.
- Ponceau_S imagefile "Ponceau S.svg".
- Ponceau_S imagesize "200".
- Ponceau_S subject Category:Azo_dyes.
- Ponceau_S subject Category:Protein_methods.
- Ponceau_S type Ability105616246.
- Ponceau_S type Abstraction100002137.
- Ponceau_S type AzoDye114770500.
- Ponceau_S type AzoDyes.
- Ponceau_S type Cognition100023271.
- Ponceau_S type ColoringMaterial114984973.
- Ponceau_S type Dye114985383.
- Ponceau_S type Know-how105616786.
- Ponceau_S type Material114580897.
- Ponceau_S type Matter100020827.
- Ponceau_S type Method105660268.
- Ponceau_S type Part113809207.
- Ponceau_S type PhysicalEntity100001930.
- Ponceau_S type ProteinMethods.
- Ponceau_S type PsychologicalFeature100023100.
- Ponceau_S type Relation100031921.
- Ponceau_S type Substance100019613.
- Ponceau_S type ChemicalCompound.
- Ponceau_S type ChemicalSubstance.
- Ponceau_S type ChemicalObject.
- Ponceau_S type Thing.
- Ponceau_S comment "Ponceau S, Acid Red 112, or C.I. 27195 is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting), as well as on cellulose acetate membranes.".
- Ponceau_S label "Ponceau S".
- Ponceau_S label "Ponceau S".
- Ponceau_S sameAs Ponceau_S.
- Ponceau_S sameAs m.08zdmh.
- Ponceau_S sameAs Q2103492.
- Ponceau_S sameAs Q2103492.
- Ponceau_S sameAs Ponceau_S.
- Ponceau_S wasDerivedFrom Ponceau_S?oldid=598998209.
- Ponceau_S depiction Ponceau_S.svg.
- Ponceau_S isPrimaryTopicOf Ponceau_S.
