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- Protein_tag abstract "Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Affinity tags are appended to proteins so that they can be purified from their crude biological source using an affinity technique. These include chitin binding protein (CBP), maltose binding protein (MBP), and glutathione-S-transferase (GST). The poly(His) tag is a widely used protein tag; it binds to metal matrices.Solubilization tags are used, especially for recombinant proteins expressed in chaperone-deficient species such as E. coli, to assist in the proper folding in proteins and keep them from precipitating. These include thioredoxin (TRX) and poly(NANP). Some affinity tags have a dual role as a solubilization agent, such as MBP, and GST.Chromatography tags are used to alter chromatographic properties of the protein to afford different resolution across a particular separation technique. Often, these consist of polyanionic amino acids, such as FLAG-tag.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, and HA-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.Fluorescence tags are used to give visual readout on a protein. GFP and its variants are the most commonly used fluorescence tags. More advanced applications of GFP include using it as a folding reporter (fluorescent if folded, colorless if not).Protein tags find many other usages, such as specific enzymatic modification (such as biotin ligase tags) and chemical modification (FlAsH) tag. Often tags are combined to produce multifunctional modifications of the protein. However, with the addition of each tag comes the risk that the native function of the protein may be abolished or compromised by interactions with the tag. Therefore, after purification, tags are commonly removed by specific proteolysis (e.g. by TEV protease, Thrombin, Factor Xa or Enteropeptidase)".
- Protein_tag wikiPageExternalLink 20235501.
- Protein_tag wikiPageExternalLink 22366317.
- Protein_tag wikiPageExternalLink 2013IDP0028R.pdf.
- Protein_tag wikiPageID "2520961".
- Protein_tag wikiPageRevisionID "600774408".
- Protein_tag hasPhotoCollection Protein_tag.
- Protein_tag subject Category:Biochemistry_methods.
- Protein_tag subject Category:Laboratory_techniques.
- Protein_tag subject Category:Molecular_biology.
- Protein_tag subject Category:Proteins.
- Protein_tag type Ability105616246.
- Protein_tag type Abstraction100002137.
- Protein_tag type BiochemistryMethods.
- Protein_tag type Chemical114806838.
- Protein_tag type Cognition100023271.
- Protein_tag type Compound114818238.
- Protein_tag type Know-how105616786.
- Protein_tag type LaboratoryTechniques.
- Protein_tag type Macromolecule114944888.
- Protein_tag type Material114580897.
- Protein_tag type Matter100020827.
- Protein_tag type Method105660268.
- Protein_tag type Molecule114682133.
- Protein_tag type OrganicCompound114727670.
- Protein_tag type Part113809207.
- Protein_tag type PhysicalEntity100001930.
- Protein_tag type Protein114728724.
- Protein_tag type Proteins.
- Protein_tag type PsychologicalFeature100023100.
- Protein_tag type Relation100031921.
- Protein_tag type Substance100019613.
- Protein_tag type Technique105665146.
- Protein_tag type Thing100002452.
- Protein_tag type Unit109465459.
- Protein_tag comment "Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Affinity tags are appended to proteins so that they can be purified from their crude biological source using an affinity technique. These include chitin binding protein (CBP), maltose binding protein (MBP), and glutathione-S-transferase (GST).".
- Protein_tag label "Protein tag".
- Protein_tag label "Protein-Tag".
- Protein_tag label "Znacznik (inżynieria genetyczna)".
- Protein_tag label "Étiquette (biologie)".
- Protein_tag label "タンパク質タグ".
- Protein_tag sameAs Protein-Tag.
- Protein_tag sameAs Étiquette_(biologie).
- Protein_tag sameAs タンパク質タグ.
- Protein_tag sameAs Znacznik_(inżynieria_genetyczna).
- Protein_tag sameAs m.07kmy7.
- Protein_tag sameAs Q645590.
- Protein_tag sameAs Q645590.
- Protein_tag sameAs Protein_tag.
- Protein_tag wasDerivedFrom Protein_tag?oldid=600774408.
- Protein_tag isPrimaryTopicOf Protein_tag.