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- Pyrosequencing abstract "Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle. It differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release on nucleotide incorporation, rather than chain termination with dideoxynucleotides. The technique was developed by Mostafa Ronaghi and Pål Nyrén at the Royal Institute of Technology in Stockholm in 1996.The desired DNA sequence is able to be determined by light emitted upon incorporation of the next complementary nucleotide by the fact that only one out of four of the possible A/T/C/G nucleotides are added and available at a time so that only one letter can be incorporated on the single stranded template (which is the sequence to be determined). The intensity of the light determines if there are more than one of these "letters" in a row. The previous nucleotide letter (one out of four possible dNTP) is degraded before the next nucleotide letter is added for synthesis: allowing for the possible revealing of the next nucleotide(s) via the resulting intensity of light (if the nucleotide added was the next complementary letter in the sequence). This process is repeated with each of the four letters until the DNA sequence of the single stranded template is determined.".
- Pyrosequencing thumbnail Pyrogram1.jpg?width=300.
- Pyrosequencing wikiPageExternalLink 3.pdf.
- Pyrosequencing wikiPageID "1154853".
- Pyrosequencing wikiPageRevisionID "592882453".
- Pyrosequencing hasPhotoCollection Pyrosequencing.
- Pyrosequencing subject Category:Biotechnology.
- Pyrosequencing subject Category:DNA_sequencing.
- Pyrosequencing subject Category:Molecular_biology.
- Pyrosequencing comment "Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle. It differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release on nucleotide incorporation, rather than chain termination with dideoxynucleotides.".
- Pyrosequencing label "Pirosecuenciación".
- Pyrosequencing label "Pyrosequencing".
- Pyrosequencing label "Pyroséquençage".
- Pyrosequencing label "Пиросеквенирование".
- Pyrosequencing label "سلسلة البايرو".
- Pyrosequencing label "焦磷酸测序".
- Pyrosequencing sameAs Pyrosekvenování.
- Pyrosequencing sameAs Pirosecuenciación.
- Pyrosequencing sameAs Pyroséquençage.
- Pyrosequencing sameAs Pyrosequencing.
- Pyrosequencing sameAs m.04bw66.
- Pyrosequencing sameAs Q2342248.
- Pyrosequencing sameAs Q2342248.
- Pyrosequencing wasDerivedFrom Pyrosequencing?oldid=592882453.
- Pyrosequencing depiction Pyrogram1.jpg.
- Pyrosequencing isPrimaryTopicOf Pyrosequencing.