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- Reverse_transcription_polymerase_chain_reaction abstract "Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR). This technique is commonly used in molecular biology to detect RNA expression levels. RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and scientists alike. However, they are separate and distinct techniques. While RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA (cDNA) transcripts from RNA, qPCR is used to quantitatively measure the amplification of DNA using fluorescent probes. qPCR is also referred to as quantitative PCR, quantitative real-time PCR, and real-time quantitative PCR.Although RT-PCR and the traditional PCR both produce multiple copies of particular DNA isolates through amplification, the applications of the two techniques are fundamentally different. The traditional PCR is simply used to exponentially amplify given DNA sequences. RT-PCR is used to clone expressed genes by reverse transcribing the RNA of interest into its DNA complement through the use of reverse transcriptase. Subsequently, the newly synthesized cDNA is amplified using traditional PCR.In addition to qualitatively study gene expression, quantitative PCR can be utilized for quantification of RNA, in both relative and absolute terms, by incorporating qPCR into the technique. The combined technique, described as quantitative RT-PCR or real-time RT-PCR (sometimes even quantitative real-time RT-PCR), is often abbreviated as qRT-PCR, RT-qPCR, or RRT-PCR. Compared to other RNA quantification methods, such as northern blot, qRT-PCR is considered to be the most powerful, sensitive, and quantitative assay for the detection of RNA levels. It is frequently used in the expression analysis of single or multiple genes, and expression patterns for identifying infections and diseases.In order to avoid confusion, the following abbreviations will be used consistently throughout this article:".
- Reverse_transcription_polymerase_chain_reaction thumbnail Reverse_transcription_polymerase_chain_reaction.jpg?width=300.
- Reverse_transcription_polymerase_chain_reaction wikiPageExternalLink RT_PCR.html.
- Reverse_transcription_polymerase_chain_reaction wikiPageExternalLink jvhpcr.html.
- Reverse_transcription_polymerase_chain_reaction wikiPageExternalLink list.aspx?iid=93.
- Reverse_transcription_polymerase_chain_reaction wikiPageExternalLink www.realtimeprimers.org.
- Reverse_transcription_polymerase_chain_reaction wikiPageID "235077".
- Reverse_transcription_polymerase_chain_reaction wikiPageRevisionID "605055916".
- Reverse_transcription_polymerase_chain_reaction hasPhotoCollection Reverse_transcription_polymerase_chain_reaction.
- Reverse_transcription_polymerase_chain_reaction subject Category:Laboratory_techniques.
- Reverse_transcription_polymerase_chain_reaction subject Category:Molecular_biology.
- Reverse_transcription_polymerase_chain_reaction subject Category:Polymerase_chain_reaction.
- Reverse_transcription_polymerase_chain_reaction type Ability105616246.
- Reverse_transcription_polymerase_chain_reaction type Abstraction100002137.
- Reverse_transcription_polymerase_chain_reaction type Cognition100023271.
- Reverse_transcription_polymerase_chain_reaction type Know-how105616786.
- Reverse_transcription_polymerase_chain_reaction type LaboratoryTechniques.
- Reverse_transcription_polymerase_chain_reaction type Method105660268.
- Reverse_transcription_polymerase_chain_reaction type PsychologicalFeature100023100.
- Reverse_transcription_polymerase_chain_reaction type Technique105665146.
- Reverse_transcription_polymerase_chain_reaction comment "Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR). This technique is commonly used in molecular biology to detect RNA expression levels. RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and scientists alike. However, they are separate and distinct techniques.".
- Reverse_transcription_polymerase_chain_reaction label "PCR z odwrotną transkryptazą".
- Reverse_transcription_polymerase_chain_reaction label "RT-PCR".
- Reverse_transcription_polymerase_chain_reaction label "RT-PCR".
- Reverse_transcription_polymerase_chain_reaction label "Reverse Transkriptase-Polymerase-Kettenreaktion".
- Reverse_transcription_polymerase_chain_reaction label "Reverse transcriptase-polymerase chain reaction".
- Reverse_transcription_polymerase_chain_reaction label "Reverse transcription polymerase chain reaction".
- Reverse_transcription_polymerase_chain_reaction label "ПЦР с обратной транскрипцией".
- Reverse_transcription_polymerase_chain_reaction label "逆転写ポリメラーゼ連鎖反応".
- Reverse_transcription_polymerase_chain_reaction label "逆轉錄聚合酶鏈式反應".
- Reverse_transcription_polymerase_chain_reaction sameAs Reverse_Transkriptase-Polymerase-Kettenreaktion.
- Reverse_transcription_polymerase_chain_reaction sameAs RT-PCR.
- Reverse_transcription_polymerase_chain_reaction sameAs Reverse_transcriptase-polymerase_chain_reaction.
- Reverse_transcription_polymerase_chain_reaction sameAs 逆転写ポリメラーゼ連鎖反応.
- Reverse_transcription_polymerase_chain_reaction sameAs 역전사_중합효소_연쇄반응.
- Reverse_transcription_polymerase_chain_reaction sameAs PCR_z_odwrotną_transkryptazą.
- Reverse_transcription_polymerase_chain_reaction sameAs RT-PCR.
- Reverse_transcription_polymerase_chain_reaction sameAs m.01j4yg.
- Reverse_transcription_polymerase_chain_reaction sameAs Q169526.
- Reverse_transcription_polymerase_chain_reaction sameAs Q169526.
- Reverse_transcription_polymerase_chain_reaction sameAs Reverse_transcription_polymerase_chain_reaction.
- Reverse_transcription_polymerase_chain_reaction wasDerivedFrom Reverse_transcription_polymerase_chain_reaction?oldid=605055916.
- Reverse_transcription_polymerase_chain_reaction depiction Reverse_transcription_polymerase_chain_reaction.jpg.
- Reverse_transcription_polymerase_chain_reaction isPrimaryTopicOf Reverse_transcription_polymerase_chain_reaction.
