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- SB_buffer abstract "SB buffer is a buffer solution used in agarose and polyacrylamide gel electrophoresis for the separation of nucleic acids such as DNA and RNA. "SB" is a commercial trademark of Faster Better Media LLC for their sodium boric acid-based conductive medium (US Patent # 7811437), which is based on the publications of Brody and Kern.It is made up of sodium borate, usually 1–10 mM at pH 8.0. It has a lower conductivity, produces sharper bands, and can be run at higher speeds than can gels made from TBE buffer or TAE buffer (5–35 V/cm as compared to 5–10 V/cm). At a given voltage, heat will be generated and thus the gel will be heated. However, SB buffer has lower conductivity than TBE and TAE, and thus the gel temperature is much lower than with TBE or TAE buffers. Therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time. Downstream applications, such as isolation of DNA from a gel slice or southern blot analysis, work as expected with sodium borate gels. LB buffer containing lithium borate is similar to sodium borate and has all of its advantages, but permits use of even higher voltages due to the lower conductivity of lithium ions as compared to sodium ions. However, lithium borate is somewhat more expensive.".
- SB_buffer wikiPageExternalLink borate.html.
- SB_buffer wikiPageExternalLink showFullText?submitFullText=Full+Text+HTML&doi=10.1111%2Fj.1471-8286.2006.01290.x.
- SB_buffer wikiPageExternalLink science?_ob=ArticleURL&_udi=B6W9V-4G69BVP-6&_user=4250348&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000052423&_version=1&_urlVersion=0&_userid=4250348&md5=fed66d13a7793754c3f81d6fcb59cb52.
- SB_buffer wikiPageID "14841127".
- SB_buffer wikiPageRevisionID "519604718".
- SB_buffer hasPhotoCollection SB_buffer.
- SB_buffer subject Category:Buffers.
- SB_buffer type Abstraction100002137.
- SB_buffer type Buffer114785941.
- SB_buffer type Buffers.
- SB_buffer type Chemical114806838.
- SB_buffer type Compound114818238.
- SB_buffer type Material114580897.
- SB_buffer type Matter100020827.
- SB_buffer type Part113809207.
- SB_buffer type PhysicalEntity100001930.
- SB_buffer type Relation100031921.
- SB_buffer type Substance100019613.
- SB_buffer comment "SB buffer is a buffer solution used in agarose and polyacrylamide gel electrophoresis for the separation of nucleic acids such as DNA and RNA. "SB" is a commercial trademark of Faster Better Media LLC for their sodium boric acid-based conductive medium (US Patent # 7811437), which is based on the publications of Brody and Kern.It is made up of sodium borate, usually 1–10 mM at pH 8.0.".
- SB_buffer label "SB buffer".
- SB_buffer sameAs m.03gzszq.
- SB_buffer sameAs Q7389027.
- SB_buffer sameAs Q7389027.
- SB_buffer sameAs SB_buffer.
- SB_buffer wasDerivedFrom SB_buffer?oldid=519604718.
- SB_buffer isPrimaryTopicOf SB_buffer.