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- SDD-AGE abstract "SDD-AGE is short for Semi-Denaturating Detergent Agarose Gel Electrophoresis. This is a method for detecting and characterizing large protein polymers which are stable in 2% SDS at room temperature, unlike most large protein complexes. This method is very useful for studying prions and amyloids, which are characterized by the formation of proteinaceous polymers. Agarose is used for the gel since the SDS-resistant polymers are large (in the 200-4000+ КDa range) and cannot enter a conventional polyacrylamide gel, which has small pores. Agarose on the other hand has large pores, which allows for the separation of polymers.Use of this method allowed researchers to understand that at least some types of prion aggregates existed in a two-level structure - protein molecules grouped into polymers, which are very stable and withstand treatment with 2% SDS at room temperature, and aggregates, which are bundles of polymers, that dissociate under these conditions. Differences in the size of polymers can indicate the efficiency of polymer fragmentation in vivo.".
- SDD-AGE thumbnail SDD-AGE_(small_and_large_polymers).jpg?width=300.
- SDD-AGE wikiPageID "26964637".
- SDD-AGE wikiPageRevisionID "532115375".
- SDD-AGE hasPhotoCollection SDD-AGE.
- SDD-AGE subject Category:Molecular_biology.
- SDD-AGE subject Category:Protein_methods.
- SDD-AGE type Ability105616246.
- SDD-AGE type Abstraction100002137.
- SDD-AGE type Cognition100023271.
- SDD-AGE type Know-how105616786.
- SDD-AGE type Method105660268.
- SDD-AGE type ProteinMethods.
- SDD-AGE type PsychologicalFeature100023100.
- SDD-AGE comment "SDD-AGE is short for Semi-Denaturating Detergent Agarose Gel Electrophoresis. This is a method for detecting and characterizing large protein polymers which are stable in 2% SDS at room temperature, unlike most large protein complexes. This method is very useful for studying prions and amyloids, which are characterized by the formation of proteinaceous polymers.".
- SDD-AGE label "SDD-AGE".
- SDD-AGE label "SDD-AGE".
- SDD-AGE sameAs SDD-AGE.
- SDD-AGE sameAs m.0cnzqfn.
- SDD-AGE sameAs Q7389421.
- SDD-AGE sameAs Q7389421.
- SDD-AGE sameAs SDD-AGE.
- SDD-AGE wasDerivedFrom SDD-AGE?oldid=532115375.
- SDD-AGE depiction SDD-AGE_(small_and_large_polymers).jpg.
- SDD-AGE isPrimaryTopicOf SDD-AGE.