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- Spin_column-based_nucleic_acid_purification abstract "Column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids.This method relies on the fact that the nucleic acid may bind (adsorption) to the solid phase (silica or other) depending on the pH and the salt content of the buffer, which may be a Tris-EDTA (TE) buffer or Phosphate buffer (used in DNA microarray experiments due to the reactive amine groups in tris). Therefore, three stages are:The sample is added to the column and the nucleic acid binds due to the lower pH (relative to the silanol groups on the column) and salt concentration of the binding solution, which may contain buffer, a denaturing agent (such as guanidine hydrochloride), Triton X-100, isopropanol and a pH indicatorThe column is then washed (5 mM KPO4 pH 8.0 or similar, 80% EtOH)The column can be eluted with buffer or simply waterEven prior to the major techniques employed today it was known that in the presence of chaotropic agents, such as sodium iodide or sodium perchlorate, DNA binds to silica, glass particles or to unicellular algae called diatoms which shield their cell walls with silica. This property was used to purify nucleic acid using glass powder or silica beads under alkaline conditions. This was later improved used guanidinium thiocyanate or guanidinium hydrochloride as the chaotropic agent. The use of beads was later changed to minicolumns.For explanation of how the silica and DNA bind see separation by silica adsorption↑ ↑".
- Spin_column-based_nucleic_acid_purification thumbnail Qiagen_Mini_Spin_Column.svg?width=300.
- Spin_column-based_nucleic_acid_purification wikiPageID "17314163".
- Spin_column-based_nucleic_acid_purification wikiPageRevisionID "551525813".
- Spin_column-based_nucleic_acid_purification hasPhotoCollection Spin_column-based_nucleic_acid_purification.
- Spin_column-based_nucleic_acid_purification subject Category:Biochemistry_methods.
- Spin_column-based_nucleic_acid_purification subject Category:Molecular_biology.
- Spin_column-based_nucleic_acid_purification type Ability105616246.
- Spin_column-based_nucleic_acid_purification type Abstraction100002137.
- Spin_column-based_nucleic_acid_purification type BiochemistryMethods.
- Spin_column-based_nucleic_acid_purification type Cognition100023271.
- Spin_column-based_nucleic_acid_purification type Know-how105616786.
- Spin_column-based_nucleic_acid_purification type Method105660268.
- Spin_column-based_nucleic_acid_purification type PsychologicalFeature100023100.
- Spin_column-based_nucleic_acid_purification comment "Column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids.This method relies on the fact that the nucleic acid may bind (adsorption) to the solid phase (silica or other) depending on the pH and the salt content of the buffer, which may be a Tris-EDTA (TE) buffer or Phosphate buffer (used in DNA microarray experiments due to the reactive amine groups in tris).".
- Spin_column-based_nucleic_acid_purification label "Colonne de biologie moléculaire".
- Spin_column-based_nucleic_acid_purification label "Spin column-based nucleic acid purification".
- Spin_column-based_nucleic_acid_purification sameAs Colonne_de_biologie_moléculaire.
- Spin_column-based_nucleic_acid_purification sameAs m.043lfns.
- Spin_column-based_nucleic_acid_purification sameAs Q2984312.
- Spin_column-based_nucleic_acid_purification sameAs Q2984312.
- Spin_column-based_nucleic_acid_purification sameAs Spin_column-based_nucleic_acid_purification.
- Spin_column-based_nucleic_acid_purification wasDerivedFrom Spin_column-based_nucleic_acid_purification?oldid=551525813.
- Spin_column-based_nucleic_acid_purification depiction Qiagen_Mini_Spin_Column.svg.
- Spin_column-based_nucleic_acid_purification isPrimaryTopicOf Spin_column-based_nucleic_acid_purification.
