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- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment abstract "Systematic Evolution of Ligands by Exponential Enrichment (SELEX), also referred to as in vitro selection or in vitro evolution, is a combinatorial chemistry technique in molecular biology for producing oligonucleotides of either single-stranded DNA or RNA that specifically bind to a target ligand or ligands. Although SELEX has emerged as the most commonly used name for the procedure, some researchers have referred to it as SAAB (selected and amplified binding site) and CASTing (Cyclic amplification and selection of targets)The process begins with the synthesis of a very large oligonucleotide library consisting of randomly generated sequences of fixed length flanked by constant 5' and 3' ends that serve as primers. For a randomly generated region of length n, the number of possible sequences in the library is 4n (n positions with four possibilities (A,T,C,G) at each position). The sequences in the library are exposed to the target ligand - which may be a protein or a small organic compound - and those that do not bind the target are removed, usually by affinity chromatography. The bound sequences are eluted and amplified by PCR to prepare for subsequent rounds of selection in which the stringency of the elution conditions is increased to identify the tightest-binding sequences. An advancement on the original method allows an RNA library to omit the constant primer regions, which can be difficult to remove after the selection process because they stabilize secondary structures that are unstable when formed by the random region alone.The technique has been used to evolve aptamers of extremely high binding affinity to a variety of target ligands, including small molecules such as ATP and adenosine and proteins such as prions and vascular endothelial growth factor (VEGF). Clinical uses of the technique are suggested by aptamers that bind tumor markers and a VEGF-binding aptamer trade-named Macugen has been approved by the FDA for treatment of macular degeneration.One caution advanced in relation to the method emphasizes that selection for extremely high, sub-nanomolar binding affinities may not in fact improve specificity for the target molecule. Off-target binding to related molecules could have significant clinical effects.".
- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment thumbnail Selex1.svg?width=300.
- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment wikiPageExternalLink aptamer.freebase.com.
- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment wikiPageID "8028338".
- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment wikiPageRevisionID "606603096".
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- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment subject Category:Evolution.
- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment subject Category:Molecular_biology.
- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment comment "Systematic Evolution of Ligands by Exponential Enrichment (SELEX), also referred to as in vitro selection or in vitro evolution, is a combinatorial chemistry technique in molecular biology for producing oligonucleotides of either single-stranded DNA or RNA that specifically bind to a target ligand or ligands.".
- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment label "Systematic Evolution of Ligands by Exponential Enrichment".
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- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment wasDerivedFrom Systematic_Evolution_of_Ligands_by_Exponential_Enrichment?oldid=606603096.
- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment depiction Selex1.svg.
- Systematic_Evolution_of_Ligands_by_Exponential_Enrichment isPrimaryTopicOf Systematic_Evolution_of_Ligands_by_Exponential_Enrichment.
