Matches in DBpedia 2014 for { <http://dbpedia.org/resource/TBE_buffer> ?p ?o. }
Showing items 1 to 34 of
34
with 100 items per page.
- TBE_buffer abstract "TBE or Tris/Borate/EDTA, is a buffer solution containing a mixture of Tris base, boric acid and EDTA.In molecular biology, TBE and TAE buffers are often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg2+ is also a co-factor for many useful DNA-modifying enzymes such as restriction enzymes and DNA polymerases, its concentration in TBE or TAE buffers is generally kept low (typically at around 1 mM).Recently, Brody & Kern simplified electrophoretic buffers by substituting TBE and TAE buffers for a more efficient and inexpensive conductive media in gel systems.".
- TBE_buffer wikiPageID "2257664".
- TBE_buffer wikiPageRevisionID "606074250".
- TBE_buffer hasPhotoCollection TBE_buffer.
- TBE_buffer subject Category:Buffers.
- TBE_buffer type Abstraction100002137.
- TBE_buffer type Buffer114785941.
- TBE_buffer type Buffers.
- TBE_buffer type Chemical114806838.
- TBE_buffer type Compound114818238.
- TBE_buffer type Material114580897.
- TBE_buffer type Matter100020827.
- TBE_buffer type Part113809207.
- TBE_buffer type PhysicalEntity100001930.
- TBE_buffer type Relation100031921.
- TBE_buffer type Substance100019613.
- TBE_buffer comment "TBE or Tris/Borate/EDTA, is a buffer solution containing a mixture of Tris base, boric acid and EDTA.In molecular biology, TBE and TAE buffers are often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+).".
- TBE_buffer label "Buffer TBE".
- TBE_buffer label "TBE buffer".
- TBE_buffer label "TBE-Puffer".
- TBE_buffer label "Tampon TBE".
- TBE_buffer label "Tampão TBE".
- TBE_buffer label "Tampón TBE".
- TBE_buffer sameAs TBE-Puffer.
- TBE_buffer sameAs Tampón_TBE.
- TBE_buffer sameAs Tampon_TBE.
- TBE_buffer sameAs Buffer_TBE.
- TBE_buffer sameAs Tampão_TBE.
- TBE_buffer sameAs m.06zswh.
- TBE_buffer sameAs Q369325.
- TBE_buffer sameAs Q369325.
- TBE_buffer sameAs TBE_buffer.
- TBE_buffer wasDerivedFrom TBE_buffer?oldid=606074250.
- TBE_buffer isPrimaryTopicOf TBE_buffer.