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- TSE_buffer abstract "TSE or Tris/Sucrose/EDTA, is a buffer solution containing a mixture of Tris base, sucrose and EDTA.In molecular biology, TSE buffers are often used in procedures involving nucleic acids. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. The concentration of tris in the solution is kept near 25 mM. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg2+ is also a co-factor for many useful DNA-modifying enzymes such as restriction enzymes and DNA polymerases, its concentration in TSE buffers is generally kept low (typically at around 2.5 mM). The sucrose is generally kept at a concentration of 0.25 M to 0.3 M.".
- TSE_buffer wikiPageID "22600725".
- TSE_buffer wikiPageRevisionID "286641087".
- TSE_buffer hasPhotoCollection TSE_buffer.
- TSE_buffer subject Category:Buffers.
- TSE_buffer type Abstraction100002137.
- TSE_buffer type Buffer114785941.
- TSE_buffer type Buffers.
- TSE_buffer type Chemical114806838.
- TSE_buffer type Compound114818238.
- TSE_buffer type Material114580897.
- TSE_buffer type Matter100020827.
- TSE_buffer type Part113809207.
- TSE_buffer type PhysicalEntity100001930.
- TSE_buffer type Relation100031921.
- TSE_buffer type Substance100019613.
- TSE_buffer comment "TSE or Tris/Sucrose/EDTA, is a buffer solution containing a mixture of Tris base, sucrose and EDTA.In molecular biology, TSE buffers are often used in procedures involving nucleic acids. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. The concentration of tris in the solution is kept near 25 mM. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+).".
- TSE_buffer label "TSE buffer".
- TSE_buffer sameAs m.05zjhk7.
- TSE_buffer sameAs Q7671578.
- TSE_buffer sameAs Q7671578.
- TSE_buffer sameAs TSE_buffer.
- TSE_buffer wasDerivedFrom TSE_buffer?oldid=286641087.
- TSE_buffer isPrimaryTopicOf TSE_buffer.