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catalog contributor b3491692.
catalog created "c1991.".
catalog date "1991".
catalog date "c1991.".
catalog dateCopyrighted "c1991.".
catalog description "Chromosome-mediated gene transfer / Janet M. Shipley, Denise Sheer -- Manipulation of adenovirus vectors / Frank L. Graham, Ludvik Prevec -- Manipulation of vaccinia virus vectors / Michael Mackett -- Manipulation of baculovirus vectors / Murk J. Bailey, Robert D. Possee -- Manipulation of SV40 vectors / Marie-Louise Hammurskjöld -- Choice and manipulation of retroviral vectors / Jay P. Morgenstern, Hartmut Land -- Use of tissue-plasminogen activator as a reporter gene / Nicholas C. Wrighton -- Use of Escherichia coli (E. coli) lacZ ([beta]-galactosidase) as a reporter gene / Grant R. MacGregor, Gamy P. Nolan, Steven Fiering, Mario Roederer, Leonard A. Herzenberg -- Application of the firefly luciferase reporter gene / Vincent Giguère -- Use of vectors to confer resistance to antibiotics G418 and hygromycin in stably transfected cell lines / Robert F. Santerre, Jenna D. Walls, Brian W. Grinnell -- ".
catalog description "Determination of foreign gene copy number in stably transfected cell lines by Southern transfer analysis / Ronald M. Fourney, Rémy Aubin, Kevin D. Dietrich, Makolm C. Paterson -- Evaluation of extrachromosomal gene copy number of transiently transfected cell lines / Angus C. Wilson, Roger K. Patient -- Use of Dpn I restriction enzyme to assess newly replicated gene copies in amplifiable vector systems / Alison C. Brewer, Roger K. Patient -- Use of polymerase chain reaction (PCR) to detect homologous recombination in transfected cell lines / Andreas Zimmer, Peter Gruss -- Induction of erythroid-specific expression in murine erythroleukemia (MEL) cell lines / Michael Antoniou.".
catalog description "Includes bibliographical references and index.".
catalog description "Preparation of recombinant plasmid DNA for DNA-mediated gene transfer / Rémy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Calcium phosphate mediated gene transfer into established cell lines / Hiroto Okayama, Claudia Chen -- Transfection of the Chloramphenicol-acetyltransferase gene into eukaryotic cells using diethyl-aminoethyl (DEAE)-dextran / Richard A. Lake, Michael J. Owen -- Polybrene/DMSO-assisted gene transfer / Remy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Electroporation technique of DNA transfection / Sarah C. Spencer -- Irradiation and fusion gene transfer / Peter N. Goodfellow -- Direct use of [lambda] phage particles for DNA transfection / Masahiro Ishiura -- Cationic liposome-mediated transfection with Lipofectin reagent / Philip L. Felgner -- Preparation of high-molecular-weight DNA for use in DNA transfection : secondary transfections for cloning active genes by direct phenotypic selection / Michael A. Tainsky -- ".
catalog description "Selection of cells defective in pyrimidine (TK⁻) and purine (APRT⁻ and HPRT⁻ salvage : development of host strains appropriate for transfection / Mark Meuth, Janet Harwood -- Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay / Maggie E. Walmsley, Roger K. Patient -- The RNase protection assay / Mitchell H. Finer -- Primer extension analysis of mRNA isolated from transfected cell lines / Mark W. Leonard, Roger K. Patient -- Northern blot analysis of gene expression / Robb Krumlauf -- Analysis of transcriptional initiation in isolated nuclei / David Stott -- Immunoperoxidase staining of gene products in cultured cells using monoclonal antibodies / Roger Morris -- The use of flow cytometry to detect transfected gene products / Raymond Bujdoso, David Sargan, Keith Ballingall, Andrew Sanderson -- ".
catalog extent "xiii, 439 p. :".
catalog hasFormat "Gene transfer and expression protocols.".
catalog identifier "0896031780".
catalog identifier "0896032167 (pbk.)".
catalog isFormatOf "Gene transfer and expression protocols.".
catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 7.".
catalog isPartOf "Methods in molecular biology ; 7".
catalog issued "1991".
catalog issued "c1991.".
catalog language "eng".
catalog publisher "Clifton, New Jersey : Humana Press,".
catalog relation "Gene transfer and expression protocols.".
catalog subject "Gene expression Technique.".
catalog subject "Gene expression.".
catalog subject "Genetic transformation Technique.".
catalog subject "Genetic transformation.".
catalog subject "QH 506".
catalog subject "QH448.4 .G43 1991".
catalog subject "Transfection Technique.".
catalog tableOfContents "Chromosome-mediated gene transfer / Janet M. Shipley, Denise Sheer -- Manipulation of adenovirus vectors / Frank L. Graham, Ludvik Prevec -- Manipulation of vaccinia virus vectors / Michael Mackett -- Manipulation of baculovirus vectors / Murk J. Bailey, Robert D. Possee -- Manipulation of SV40 vectors / Marie-Louise Hammurskjöld -- Choice and manipulation of retroviral vectors / Jay P. Morgenstern, Hartmut Land -- Use of tissue-plasminogen activator as a reporter gene / Nicholas C. Wrighton -- Use of Escherichia coli (E. coli) lacZ ([beta]-galactosidase) as a reporter gene / Grant R. MacGregor, Gamy P. Nolan, Steven Fiering, Mario Roederer, Leonard A. Herzenberg -- Application of the firefly luciferase reporter gene / Vincent Giguère -- Use of vectors to confer resistance to antibiotics G418 and hygromycin in stably transfected cell lines / Robert F. Santerre, Jenna D. Walls, Brian W. Grinnell -- ".
catalog tableOfContents "Determination of foreign gene copy number in stably transfected cell lines by Southern transfer analysis / Ronald M. Fourney, Rémy Aubin, Kevin D. Dietrich, Makolm C. Paterson -- Evaluation of extrachromosomal gene copy number of transiently transfected cell lines / Angus C. Wilson, Roger K. Patient -- Use of Dpn I restriction enzyme to assess newly replicated gene copies in amplifiable vector systems / Alison C. Brewer, Roger K. Patient -- Use of polymerase chain reaction (PCR) to detect homologous recombination in transfected cell lines / Andreas Zimmer, Peter Gruss -- Induction of erythroid-specific expression in murine erythroleukemia (MEL) cell lines / Michael Antoniou.".
catalog tableOfContents "Preparation of recombinant plasmid DNA for DNA-mediated gene transfer / Rémy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Calcium phosphate mediated gene transfer into established cell lines / Hiroto Okayama, Claudia Chen -- Transfection of the Chloramphenicol-acetyltransferase gene into eukaryotic cells using diethyl-aminoethyl (DEAE)-dextran / Richard A. Lake, Michael J. Owen -- Polybrene/DMSO-assisted gene transfer / Remy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Electroporation technique of DNA transfection / Sarah C. Spencer -- Irradiation and fusion gene transfer / Peter N. Goodfellow -- Direct use of [lambda] phage particles for DNA transfection / Masahiro Ishiura -- Cationic liposome-mediated transfection with Lipofectin reagent / Philip L. Felgner -- Preparation of high-molecular-weight DNA for use in DNA transfection : secondary transfections for cloning active genes by direct phenotypic selection / Michael A. Tainsky -- ".
catalog tableOfContents "Selection of cells defective in pyrimidine (TK⁻) and purine (APRT⁻ and HPRT⁻ salvage : development of host strains appropriate for transfection / Mark Meuth, Janet Harwood -- Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay / Maggie E. Walmsley, Roger K. Patient -- The RNase protection assay / Mitchell H. Finer -- Primer extension analysis of mRNA isolated from transfected cell lines / Mark W. Leonard, Roger K. Patient -- Northern blot analysis of gene expression / Robb Krumlauf -- Analysis of transcriptional initiation in isolated nuclei / David Stott -- Immunoperoxidase staining of gene products in cultured cells using monoclonal antibodies / Roger Morris -- The use of flow cytometry to detect transfected gene products / Raymond Bujdoso, David Sargan, Keith Ballingall, Andrew Sanderson -- ".
catalog title "Gene transfer and expression protocols / edited by E.J. Murray.".
catalog type "text".