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• catalog contributor b5825861.
• catalog created "c1984.".
• catalog date "1984".
• catalog date "c1984.".
• catalog dateCopyrighted "c1984.".
• catalog description "In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system / C.L. Oliver, C.D. Boyd -- Immunoprecipitation of in vitro translation products with protein A bound to sepharose / C.L. Olliver, C.D. Boyd -- In vitro continuation of RNA synthesis initiated in vivo / Theodore Gurney -- Synthesis of double-stranded complementary DNA from poly(A)+mRNA / R. McGookin -- Plasmid DNA isolation by the cleared lysate method / Stephen A. Boffey -- Plasmid DNA isolation (sheared lysate method) / J.W. Dale, P.J. Greenaway -- Small-scale plasmid DNA preparation / J.W. Dale, P.J. Greenaway -- Preparation of chromosomal DNA from E. coli / J.W. Dale, P.J. Greenaway -- Preparation and assay of phage lambda / J.W. Dale, P.J. Greenaway -- Preparation of phage lambda DNA / J.W. Dale, P.J. Greenaway -- The use of restriction endonucleases / Elliot B. Gingold -- Ligation of DNA with T4 DNA ligase / Wim Gaastra, Kirsten Hansen -- ".
• catalog description "Includes bibliographical references and index.".
• catalog description "One-dimensional electrophoresis of nucleic acids in agarose using denaturation with formaldehyde and identification of 3H-labeled RNA by fluorography / Theodore Gurney -- Gel electrophoresis of RNA in agarose and polyacrylamide under nondenaturing conditions / R. McGookin -- The extraction of Total RNA by the detergent and phenol method / Robert J. Slater -- RNA extraction by the proteinase K method / R. McGookin -- RNA extraction by the guanidine thiocyanate procedure / R. McGookin -- The purification of poly(A)-containing RNA by affinity chromatography / Robert J. Slater -- Messenger RNA fractionation on neutral sucrose gradients / R. McGookin -- The estimation of mRNA content by Poly(U) hybridization / Robert J. Slater -- DNA directed in vitro protein synthesis with Escherichia coli S-30 extracts / Jytte Josephsen, Wim Gaastra -- In vitro translation of messenger RNA in a wheat germ extract cell-free system / C.L. Olliver, A. Grobler-Rabie, C.D. Boyd -- ".
• catalog description "The Burton assay for DNA / Jaap H. Waterborg, Harry R. Matthews -- DABA fluorescence assay for submicrogram amounts of DNA / Theodore Gurney, Elizabeth G. Gurney -- Preparation of "RNase-Free" DNase by alkylation / Theodore Gurney, Elizabeth G. Gurney -- The isolation of satellite DNA by density gradient centrifugation / Craig A. Cooney, Harry R. Matthews -- The isolation of high molecular weight eukaryotic DNA / C.G. Mathew -- Preparation of lyophilized cells to preserve enzyme activities and high molecular weight nucleic acids / Theodore Gurney -- Agarose gel electrophoresis of DNA / Stephen A. Boffey -- Autoradiography of gels containing 32P / Verena D. Huebner, Harry R. Matthews -- Detection of specific DNA sequences : the Southern transfer / C.G.P. Mathew -- The extraction and isolation of DNA from gels / Wim Gaastra, Per Linå Jørgensen -- ".
• catalog description "The isolation of minicells / Wim Gaastra, Per Klemm -- The isolation of maxicells / Wim Gaastra, Per Klemm -- The identification of gene products in minicells and maxicells / Wim Gaastra, Per Klemm -- Molecular cloning in bacteriophage lambda and in cosmids / Claus Christiansen -- DNA transformation of mammalian cells / Jeffrey W. Pollard, Yunus Luqmani, Alan Bateson, Kokila Chotai -- Chemical cleavage (Maxam and Gilbert) method for DNA sequence determination / Wim Gaastra -- Gel electrophoretic analysis of DNA sequencing products / Wim Gaastra -- DNA sequence determination using dideoxy analogs / M.J. Owen.".
• catalog description "The use of alkaline phosphatase to prevent vector regeneration / J.W. Dale, P.J. Greenaway -- Bacterial transformation / Elliot B. Gingold -- Bacterial transformation (Kushner method) / J.W. Dale, P.J. Greenaway -- In vitro packaging of DNA / J.W. Dale, P.J. Greenaway -- Yeast transformation / Elliot B. Gingold -- Radiolabeling of DNA by nick translation / C.G. Mathew -- Radiolabeling of DNA using polynucleotide kinase / Wim Gaastra, Jytte Josephsen -- Radiolabeling of DNA with 3' terminal transferase / Wim Gaastra, Per Klemm -- Radiolabeling of DNA with the Klenow fragment of DNA polymerase / Wim Gaastra, Jytte Josephsen -- Identification of recombinant plasmids by in situ colony hybridization / J.W. Dale, P.J. Greenaway -- Identification of recombinant phages by plaque hybridization / J.W. Dale, P.J. Greenaway -- Plasmid screening using single colony lysates / J.W. Dale, P.J. Greenaway -- Immunological detection of gene expression in recombinant clones / J.W. Dale, P.J. Greenaway -- ".
• catalog extent "xiv, 375 p. :".
• catalog identifier "0896030644".
• catalog isPartOf "Biological methods".
• catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 2.".
• catalog isPartOf "Methods in molecular biology ; v. 2".
• catalog issued "1984".
• catalog issued "c1984.".
• catalog language "eng".
• catalog publisher "Clifton, N.J. : Humana Press,".
• catalog subject "574.87/328 20".
• catalog subject "Acides nucléiques.".
• catalog subject "Biologie moléculaire - Méthode.".
• catalog subject "Molecular biology Research Methodology.".
• catalog subject "Nucleic Acids.".
• catalog subject "Nucleic acids Analysis Methodology.".
• catalog subject "QH 506".
• catalog subject "QP620 .N7948 1984".
• catalog tableOfContents "In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system / C.L. Oliver, C.D. Boyd -- Immunoprecipitation of in vitro translation products with protein A bound to sepharose / C.L. Olliver, C.D. Boyd -- In vitro continuation of RNA synthesis initiated in vivo / Theodore Gurney -- Synthesis of double-stranded complementary DNA from poly(A)+mRNA / R. McGookin -- Plasmid DNA isolation by the cleared lysate method / Stephen A. Boffey -- Plasmid DNA isolation (sheared lysate method) / J.W. Dale, P.J. Greenaway -- Small-scale plasmid DNA preparation / J.W. Dale, P.J. Greenaway -- Preparation of chromosomal DNA from E. coli / J.W. Dale, P.J. Greenaway -- Preparation and assay of phage lambda / J.W. Dale, P.J. Greenaway -- Preparation of phage lambda DNA / J.W. Dale, P.J. Greenaway -- The use of restriction endonucleases / Elliot B. Gingold -- Ligation of DNA with T4 DNA ligase / Wim Gaastra, Kirsten Hansen -- ".
• catalog tableOfContents "One-dimensional electrophoresis of nucleic acids in agarose using denaturation with formaldehyde and identification of 3H-labeled RNA by fluorography / Theodore Gurney -- Gel electrophoresis of RNA in agarose and polyacrylamide under nondenaturing conditions / R. McGookin -- The extraction of Total RNA by the detergent and phenol method / Robert J. Slater -- RNA extraction by the proteinase K method / R. McGookin -- RNA extraction by the guanidine thiocyanate procedure / R. McGookin -- The purification of poly(A)-containing RNA by affinity chromatography / Robert J. Slater -- Messenger RNA fractionation on neutral sucrose gradients / R. McGookin -- The estimation of mRNA content by Poly(U) hybridization / Robert J. Slater -- DNA directed in vitro protein synthesis with Escherichia coli S-30 extracts / Jytte Josephsen, Wim Gaastra -- In vitro translation of messenger RNA in a wheat germ extract cell-free system / C.L. Olliver, A. Grobler-Rabie, C.D. Boyd -- ".
• catalog tableOfContents "The Burton assay for DNA / Jaap H. Waterborg, Harry R. Matthews -- DABA fluorescence assay for submicrogram amounts of DNA / Theodore Gurney, Elizabeth G. Gurney -- Preparation of "RNase-Free" DNase by alkylation / Theodore Gurney, Elizabeth G. Gurney -- The isolation of satellite DNA by density gradient centrifugation / Craig A. Cooney, Harry R. Matthews -- The isolation of high molecular weight eukaryotic DNA / C.G. Mathew -- Preparation of lyophilized cells to preserve enzyme activities and high molecular weight nucleic acids / Theodore Gurney -- Agarose gel electrophoresis of DNA / Stephen A. Boffey -- Autoradiography of gels containing 32P / Verena D. Huebner, Harry R. Matthews -- Detection of specific DNA sequences : the Southern transfer / C.G.P. Mathew -- The extraction and isolation of DNA from gels / Wim Gaastra, Per Linå Jørgensen -- ".
• catalog tableOfContents "The isolation of minicells / Wim Gaastra, Per Klemm -- The isolation of maxicells / Wim Gaastra, Per Klemm -- The identification of gene products in minicells and maxicells / Wim Gaastra, Per Klemm -- Molecular cloning in bacteriophage lambda and in cosmids / Claus Christiansen -- DNA transformation of mammalian cells / Jeffrey W. Pollard, Yunus Luqmani, Alan Bateson, Kokila Chotai -- Chemical cleavage (Maxam and Gilbert) method for DNA sequence determination / Wim Gaastra -- Gel electrophoretic analysis of DNA sequencing products / Wim Gaastra -- DNA sequence determination using dideoxy analogs / M.J. Owen.".
• catalog tableOfContents "The use of alkaline phosphatase to prevent vector regeneration / J.W. Dale, P.J. Greenaway -- Bacterial transformation / Elliot B. Gingold -- Bacterial transformation (Kushner method) / J.W. Dale, P.J. Greenaway -- In vitro packaging of DNA / J.W. Dale, P.J. Greenaway -- Yeast transformation / Elliot B. Gingold -- Radiolabeling of DNA by nick translation / C.G. Mathew -- Radiolabeling of DNA using polynucleotide kinase / Wim Gaastra, Jytte Josephsen -- Radiolabeling of DNA with 3' terminal transferase / Wim Gaastra, Per Klemm -- Radiolabeling of DNA with the Klenow fragment of DNA polymerase / Wim Gaastra, Jytte Josephsen -- Identification of recombinant plasmids by in situ colony hybridization / J.W. Dale, P.J. Greenaway -- Identification of recombinant phages by plaque hybridization / J.W. Dale, P.J. Greenaway -- Plasmid screening using single colony lysates / J.W. Dale, P.J. Greenaway -- Immunological detection of gene expression in recombinant clones / J.W. Dale, P.J. Greenaway -- ".
• catalog title "Nucleic acids / edited by John M. Walker.".
• catalog type "text".