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- catalog contributor b5901091.
- catalog created "1994.".
- catalog date "1994".
- catalog date "1994.".
- catalog dateCopyrighted "1994.".
- catalog description "Databases -- Software -- Suppliers -- Inbred, hybrid and random-bred mice -- Mouse genomic DNAs and RNAs -- Mouse genomic and cDNA libraries and clones -- Equipment and supplies.".
- catalog description "Developmental genetics and embryology of the mouse: past, present and future -- Summary of mouse development -- Setting up a colony for the production of transgenic and mutant mice -- Recovery, culture and transfer of embryos and germ cells -- In vitro manipulation of preimplantation embryos -- Production of transgenic mice -- Isolation, culture and manipulation of embryonic stem cells -- Analysis of transgenic mice -- Techniques for visualizing genes, gene products and specialized cell types.".
- catalog description "In vitro culture of eggs, embryos, primordial germ cells and teratocarcinoma cells -- Buffers and solutions -- Acidic tyrode solution for removing zonae -- Alkaline phosphatase buffers -- Alkaline phosphatase staining solution -- Avertin, anesthetic -- Bovine serum albumin (BSA) -- Ca⁺/Mg⁺⁺-free PBS -- Ca⁺⁺/Mg⁺⁺-free tyrode ringer's saline (pH 7.6-7.7) -- Hyaluronidase -- NTM buffer (NTMT without tween 20) for in situ hybridization of intact embryos with RNA probes -- NTMT buffer for in situ hybridization of intact embryos with RNA probes.".
- catalog description "Includes bibliographical references (p. 439-470) and index.".
- catalog description "Pancreatin/trypsin solution for separating germ and tissue layers -- PBSMT for immunohistochemistry of whole mount embryos -- PBT -- Phenol/chloroform solution for isolating total RNA from mouse embryos or fetal tissues -- Phosphate-buffered saline (PBS ̲-- Pronase solution -- Saline/EDTA buffer plus glucose for isolation of germ cells and tissue culture -- 20X SSC -- 20X SSPE -- TE (tris/EDTA) buffer -- 0.25% trypsin in tris-saline for tissue culture -- Trypsin/EDTA solutions -- Sources of information -- Genetic resources -- Books -- Journals.".
- catalog extent "xvii, 497 p. :".
- catalog identifier "0879693843".
- catalog issued "1994".
- catalog issued "1994.".
- catalog language "eng".
- catalog publisher "Plainview, N.Y. : Cold Spring Harbor Laboratory Press,".
- catalog subject "1995 C-524".
- catalog subject "599.32/33 20".
- catalog subject "Genetic Engineering Laboratory Manuals.".
- catalog subject "Mice Embryos Laboratory manuals.".
- catalog subject "Mice Genetic engineering Laboratory manuals.".
- catalog subject "Mice as laboratory animals.".
- catalog subject "Mice embryology laboratory manuals.".
- catalog subject "Mice genetics laboratory manuals.".
- catalog subject "Mice immunology laboratory manuals.".
- catalog subject "Mice, Transgenic embryology laboratory manuals.".
- catalog subject "Mice, Transgenic genetics Laboratory Manuals.".
- catalog subject "QL737.R6 M2468 1994".
- catalog subject "QY 25 M278 1994".
- catalog subject "Transgenic mice Laboratory manuals.".
- catalog tableOfContents "Databases -- Software -- Suppliers -- Inbred, hybrid and random-bred mice -- Mouse genomic DNAs and RNAs -- Mouse genomic and cDNA libraries and clones -- Equipment and supplies.".
- catalog tableOfContents "Developmental genetics and embryology of the mouse: past, present and future -- Summary of mouse development -- Setting up a colony for the production of transgenic and mutant mice -- Recovery, culture and transfer of embryos and germ cells -- In vitro manipulation of preimplantation embryos -- Production of transgenic mice -- Isolation, culture and manipulation of embryonic stem cells -- Analysis of transgenic mice -- Techniques for visualizing genes, gene products and specialized cell types.".
- catalog tableOfContents "In vitro culture of eggs, embryos, primordial germ cells and teratocarcinoma cells -- Buffers and solutions -- Acidic tyrode solution for removing zonae -- Alkaline phosphatase buffers -- Alkaline phosphatase staining solution -- Avertin, anesthetic -- Bovine serum albumin (BSA) -- Ca⁺/Mg⁺⁺-free PBS -- Ca⁺⁺/Mg⁺⁺-free tyrode ringer's saline (pH 7.6-7.7) -- Hyaluronidase -- NTM buffer (NTMT without tween 20) for in situ hybridization of intact embryos with RNA probes -- NTMT buffer for in situ hybridization of intact embryos with RNA probes.".
- catalog tableOfContents "Pancreatin/trypsin solution for separating germ and tissue layers -- PBSMT for immunohistochemistry of whole mount embryos -- PBT -- Phenol/chloroform solution for isolating total RNA from mouse embryos or fetal tissues -- Phosphate-buffered saline (PBS ̲-- Pronase solution -- Saline/EDTA buffer plus glucose for isolation of germ cells and tissue culture -- 20X SSC -- 20X SSPE -- TE (tris/EDTA) buffer -- 0.25% trypsin in tris-saline for tissue culture -- Trypsin/EDTA solutions -- Sources of information -- Genetic resources -- Books -- Journals.".
- catalog title "Manipulating the mouse embryo : a laboratory manual / Brigid Hogan ... [et al.].".
- catalog type "Handbooks, manuals, etc. fast".
- catalog type "text".