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- catalog abstract "In recent years, heterologous gene expression in mammalian cells has become invaluable for studying many aspects of eukaryote cell and molecular biology. This book provides key background information and detailed practical guidance on using the major expression systems currently available. The experienced authors describe laboratory protocols that they know work, and give clear technical advice, hints and tips, troubleshooting comments, and example results, all designed to ensure practical success. Topics covered include: high-efficiency gene transfer into mammalian cells; construction and characterization of vaccinia virus recombinants; use of vectors based on gene amplification; retroviral vectors; genetic manipulation of embryonic stem cells; production of transgenic rodents by microinjection of cloned DNA; genomic and expression analysis of transgenic animals; expression using a defective HSV-1 vector system; adenovirus vectors. This volume, Mammalian Systems, is one of four new Practical Approach volumes on DNA cloning which together provide a comprehensive practical manual of current recombinant DNA methodology. Mammalian Systems will prove invaluable to all researchers, from PhD students to experienced group leaders, who already express heterologous genes in mammalian systems or who plan to do so.".
- catalog contributor b7396236.
- catalog contributor b7396237.
- catalog created "c1995-".
- catalog date "1995".
- catalog date "c1995-".
- catalog dateCopyrighted "c1995-".
- catalog description "In recent years, heterologous gene expression in mammalian cells has become invaluable for studying many aspects of eukaryote cell and molecular biology. This book provides key background information and detailed practical guidance on using the major expression systems currently available. The experienced authors describe laboratory protocols that they know work, and give clear technical advice, hints and tips, troubleshooting comments, and example results, all designed to ensure practical success.".
- catalog description "Includes bibliographical references and indexes.".
- catalog description "This volume, Mammalian Systems, is one of four new Practical Approach volumes on DNA cloning which together provide a comprehensive practical manual of current recombinant DNA methodology. Mammalian Systems will prove invaluable to all researchers, from PhD students to experienced group leaders, who already express heterologous genes in mammalian systems or who plan to do so.".
- catalog description "Topics covered include: high-efficiency gene transfer into mammalian cells; construction and characterization of vaccinia virus recombinants; use of vectors based on gene amplification; retroviral vectors; genetic manipulation of embryonic stem cells; production of transgenic rodents by microinjection of cloned DNA; genomic and expression analysis of transgenic animals; expression using a defective HSV-1 vector system; adenovirus vectors.".
- catalog description "v. 1. Core techniques -- v. 2. Expression systems -- v. 3. Complex genomes -- v. 4. Mammalian systems.".
- catalog extent "4 v. :".
- catalog identifier "0199634769 (v. 1 : pbk.) :".
- catalog identifier "0199634777 (v. 1 : hbk) :".
- catalog identifier "0199634785 (v. 2 : pbk.) :".
- catalog identifier "0199634793 (v. 2 : hbk) :".
- catalog identifier "0199634807 (v. 4 : pbk)".
- catalog identifier "0199634815 (v. 4 : hbk)".
- catalog identifier "0199634823 (v. 3 : hbk)".
- catalog identifier "0199634831 (v. 3 : pbk)".
- catalog isPartOf "Practical approach series ; 148, 149, 163, 164".
- catalog issued "1995".
- catalog issued "c1995-".
- catalog language "eng".
- catalog publisher "Oxford ; New York : IRL Press at Oxford University Press,".
- catalog subject "574.87/3282 20".
- catalog subject "Cloning, Molecular".
- catalog subject "DNA, Recombinant".
- catalog subject "Molecular cloning Laboratory manuals.".
- catalog subject "QH442.2 .D59 1994".
- catalog subject "Recombinant DNA Laboratory manuals.".
- catalog tableOfContents "v. 1. Core techniques -- v. 2. Expression systems -- v. 3. Complex genomes -- v. 4. Mammalian systems.".
- catalog title "DNA cloning : a practical approach / edited by D.M. Glover and B.D. Hames.".
- catalog type "Laboratory Manuals".
- catalog type "text".