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• catalog contributor b8249896.
• catalog contributor b8249897.
• catalog contributor b8249898.
• catalog created "c1995.".
• catalog date "1995".
• catalog date "c1995.".
• catalog dateCopyrighted "c1995.".
• catalog description "Includes bibliographical references and index.".
• catalog description "Part I. Isolation of cilia and flagella. 1. Flagellar amputation and regeneration in Chlamydomonas -- 2. large-scale isolation of Chlamydomonas flagella -- 3. Isolation of cilia from Tetrahymena thermophila -- 4. Preparation of cilia and subciliary fractions from Paramecium -- 5. Isolation of Euglena flagella -- 6. Isolation of ram sperm flagella -- 7. Isolation of molluscan gill cilia, sperm flagella, and axonemes -- 8. Isolation of flagella and their membranes from sea urchin spermatozoa -- 9. Isolation of fish sperm flagella.".
• catalog description "Part II. Culture and isolation of ciliated epithelial cells and cilia. 10. Ciliogenesis of rat tracheal epithelial cells in vitro -- 11. Preparation of explant and organ cultures and single cells from airway epithelium -- 12. Induction of ciliogenesis in oviduct epithelium -- 13. Preparation of teleost rod inner and outer segments -- 14. Isolation of respiratory cilia.".
• catalog description "Part III. General methods. 15. Electrophoretic separation of dynein heavy chains -- 16. Two-dimensional separation of axonemal proteins -- 17. Analysis of flagellar calcium-binding proteins -- 18. Phosphorylation of Chlamydomonas flagellar proteins -- 19. In situ glycosylation of flagellar lipids -- 20. Detection of flagellar protein kinases on polyvinylidene fluoride membranes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis -- 21. Measurement of ATPase activity using [gamma-32P]ATP -- 2. Nonradioactive method for ATPase assays.".
• catalog description "Part IV. Structure. 23. Immunoelectron microscopy -- 24. Immunofluorescence microscopy of cilia and flagella -- 25. Scanning transmission electron microscopic analysis of the isolated dynein ATPase -- 26. High-resolution negative staining of the isolated dynein ATPase -- 27. Computer assisted analysis of flagellar structure -- 28. Preparation of cilia and flagella for thin-section transmission electron microscope analysis -- 29. Fixation of mammalian spermatozoa for electron microscopy.".
• catalog description "Part IX. Genetics and molecular tools for the analysis of cilia and flagella. 73. Transformation of Chlamydomonas reinhardtii -- 74. Insertional mutagenesis and isolation of tagged genes in Chlamydomonas -- 75. Molecular mapping of genes for flagellar proteins in Chlamydomonas -- 76. Mating and tetrad analysis in Chlamydomonas reinhardtii -- 77. Strategies for the isolation of flagellar motility and assembly mutants in Chlamydomonas -- 78. Epitope tagging of flagellar proteins -- 79. Enrichment of mRNA encoding flagellar proteins -- 80. DNA-mediated transformation in Tetrahymena -- 81. Strategies for the isolation of ciliary motility and assembly mutants in Tetrahymena -- 82. Identification of new dynein heavy-chain genes by RNA-directed polymerase chain reaction.".
• catalog description "Part V. Motors and motion analysis. 30. Reactivation of Chlamydomonas cell models -- 31. Reactivation of extracted Paramecium models -- 32. Isolation and reactivation of newt lung respiratory cilia -- 33. Reactivation of motility of demembranated sea urchin sperm flagella -- 34. High-speed video microscopy of flagella and cilia -- 35. Micromanipulation of sperm and other ciliated of flagellated single cells -- 36. Preparation and reactivation of demembranated, cytosol-free ram spermatozoa 0-- 37. Microscopic assays of flagellar dynein activity -- 38. High-resolution imaging of flagella -- 39. Flagellar surface motility: gliding and microsphere movements -- 40. Assay of Chlamydomonas phototaxis -- 41. Quantification of ciliary beat frequency and metachrony by high-speed digital video -- Part VI. Cytoskeleton. 42. Isolation and fractionation of the Tetrahymena cytoskeleton and oral apparatus -- 43. Release of the cytoskeleton and flagellar apparatus from Chlamydomonas -- 44. Purification of SF-assemblin -- 45. Purification of basal bodies and basal body complexes from Chlamydomonas reinhardtii -- 46. Preparation and reactivation of Spermatozopsis cytoskeletons -- 47. Centrin-based contractile fibers: Chromatographic purification of centrin.".
• catalog description "Part VII. Flagellar fractionation and membrane characterization. 48. Isolation of flagellar paraxonemal rod proteins -- 49. Preparation of ciliary and flagellar remnants -- 50. In vitro polymerization of tubulin from Echinoderm sperm flagellar microtubules -- 51. Methods for the isolation of tektins and sarkosyl-insoluble protofilament ribbons -- 52. Isolation of radial spoke heads from Chlamydomonas axonemes -- 53. Isolation of the dense fibers of mammalian sperm flagella -- 54. Isolation of the fibrous sheath of mammalian sperm flagella -- 55. Isolation and fractionation of ciliary membranes from Tetrahymena -- 56. Measurement of membrane potential and Na+ and H+ transport in isolated sea urchin sperm flagella and their membrane vesicles -- 57. Nonradioactive methods for labeling and identifying membrane surface proteins -- 58. Fluorescence labeling of flagellar membranes -- 59. Electrophysiology of ciliates -- 60. Isolation of Chlamydomonas mastigonemes -- 61. Ciliary membrane tubulin: isolation and fractionation -- 62. Peptide receptors in sea urchin sperm flagella.".
• catalog description "Part VIII. Assay and isolation of flagellar enzymes. 63. Assaying protein phosphatases in sperm and flagella -- 64. Flagellar adenylyl cyclases in Chlamydomonas -- 65. Isolation and characterization of sea urchin flagellar creatine kinase -- 66. Isolation and characterization of protein kinases of Paramecium cilia -- 67. Isolation of inner- and outer-arm dyneins -- 68. Separation of dynein species by high-pressure liquid chromatography -- 69. Reconstitution of dynein arms in vitro -- 70. Inhibitors of the dynein ATPase and ciliary of flagellar motility -- 71. Vanadate-mediated photolysis of dynein heavy chains -- 72. Modified nucleotides as probes for dynein.".
• catalog extent "xxxv, 603 p. :".
• catalog identifier "0122097106 (obk.)".
• catalog identifier "0125641486".
• catalog isPartOf "Methods in cell biology, 0091-679X ; v. 47".
• catalog issued "1995".
• catalog issued "c1995.".
• catalog language "eng".
• catalog publisher "San Diego : Academic Press,".
• catalog subject "Cilia and ciliary motion Laboratory manuals.".
• catalog subject "Cilia and ciliary motion Research Technique.".
• catalog subject "Cilia physiology.".
• catalog subject "Cilia ultrastructure.".
• catalog subject "Cilia.".
• catalog subject "Flagella (Microbiology) Laboratory manuals.".
• catalog subject "Flagella (Microbiology) Research Technique.".
• catalog subject "Flagella physiology.".
• catalog subject "Flagella ultrastructure.".
• catalog subject "QH585 .M472 v.47 1995".
• catalog subject "QP310.C5 C55 1995".
• catalog subject "QS 532.5.E7 C5715 1995".
• catalog subject "W1 ME9615D v.47 1995".
• catalog tableOfContents "Part I. Isolation of cilia and flagella. 1. Flagellar amputation and regeneration in Chlamydomonas -- 2. large-scale isolation of Chlamydomonas flagella -- 3. Isolation of cilia from Tetrahymena thermophila -- 4. Preparation of cilia and subciliary fractions from Paramecium -- 5. Isolation of Euglena flagella -- 6. Isolation of ram sperm flagella -- 7. Isolation of molluscan gill cilia, sperm flagella, and axonemes -- 8. Isolation of flagella and their membranes from sea urchin spermatozoa -- 9. Isolation of fish sperm flagella.".
• catalog tableOfContents "Part II. Culture and isolation of ciliated epithelial cells and cilia. 10. Ciliogenesis of rat tracheal epithelial cells in vitro -- 11. Preparation of explant and organ cultures and single cells from airway epithelium -- 12. Induction of ciliogenesis in oviduct epithelium -- 13. Preparation of teleost rod inner and outer segments -- 14. Isolation of respiratory cilia.".
• catalog tableOfContents "Part III. General methods. 15. Electrophoretic separation of dynein heavy chains -- 16. Two-dimensional separation of axonemal proteins -- 17. Analysis of flagellar calcium-binding proteins -- 18. Phosphorylation of Chlamydomonas flagellar proteins -- 19. In situ glycosylation of flagellar lipids -- 20. Detection of flagellar protein kinases on polyvinylidene fluoride membranes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis -- 21. Measurement of ATPase activity using [gamma-32P]ATP -- 2. Nonradioactive method for ATPase assays.".
• catalog tableOfContents "Part IV. Structure. 23. Immunoelectron microscopy -- 24. Immunofluorescence microscopy of cilia and flagella -- 25. Scanning transmission electron microscopic analysis of the isolated dynein ATPase -- 26. High-resolution negative staining of the isolated dynein ATPase -- 27. Computer assisted analysis of flagellar structure -- 28. Preparation of cilia and flagella for thin-section transmission electron microscope analysis -- 29. Fixation of mammalian spermatozoa for electron microscopy.".
• catalog tableOfContents "Part IX. Genetics and molecular tools for the analysis of cilia and flagella. 73. Transformation of Chlamydomonas reinhardtii -- 74. Insertional mutagenesis and isolation of tagged genes in Chlamydomonas -- 75. Molecular mapping of genes for flagellar proteins in Chlamydomonas -- 76. Mating and tetrad analysis in Chlamydomonas reinhardtii -- 77. Strategies for the isolation of flagellar motility and assembly mutants in Chlamydomonas -- 78. Epitope tagging of flagellar proteins -- 79. Enrichment of mRNA encoding flagellar proteins -- 80. DNA-mediated transformation in Tetrahymena -- 81. Strategies for the isolation of ciliary motility and assembly mutants in Tetrahymena -- 82. Identification of new dynein heavy-chain genes by RNA-directed polymerase chain reaction.".
• catalog tableOfContents "Part V. Motors and motion analysis. 30. Reactivation of Chlamydomonas cell models -- 31. Reactivation of extracted Paramecium models -- 32. Isolation and reactivation of newt lung respiratory cilia -- 33. Reactivation of motility of demembranated sea urchin sperm flagella -- 34. High-speed video microscopy of flagella and cilia -- 35. Micromanipulation of sperm and other ciliated of flagellated single cells -- 36. Preparation and reactivation of demembranated, cytosol-free ram spermatozoa 0-- 37. Microscopic assays of flagellar dynein activity -- 38. High-resolution imaging of flagella -- 39. Flagellar surface motility: gliding and microsphere movements -- 40. Assay of Chlamydomonas phototaxis -- 41. Quantification of ciliary beat frequency and metachrony by high-speed digital video -- Part VI. Cytoskeleton. 42. Isolation and fractionation of the Tetrahymena cytoskeleton and oral apparatus -- 43. Release of the cytoskeleton and flagellar apparatus from Chlamydomonas -- 44. Purification of SF-assemblin -- 45. Purification of basal bodies and basal body complexes from Chlamydomonas reinhardtii -- 46. Preparation and reactivation of Spermatozopsis cytoskeletons -- 47. Centrin-based contractile fibers: Chromatographic purification of centrin.".
• catalog tableOfContents "Part VII. Flagellar fractionation and membrane characterization. 48. Isolation of flagellar paraxonemal rod proteins -- 49. Preparation of ciliary and flagellar remnants -- 50. In vitro polymerization of tubulin from Echinoderm sperm flagellar microtubules -- 51. Methods for the isolation of tektins and sarkosyl-insoluble protofilament ribbons -- 52. Isolation of radial spoke heads from Chlamydomonas axonemes -- 53. Isolation of the dense fibers of mammalian sperm flagella -- 54. Isolation of the fibrous sheath of mammalian sperm flagella -- 55. Isolation and fractionation of ciliary membranes from Tetrahymena -- 56. Measurement of membrane potential and Na+ and H+ transport in isolated sea urchin sperm flagella and their membrane vesicles -- 57. Nonradioactive methods for labeling and identifying membrane surface proteins -- 58. Fluorescence labeling of flagellar membranes -- 59. Electrophysiology of ciliates -- 60. Isolation of Chlamydomonas mastigonemes -- 61. Ciliary membrane tubulin: isolation and fractionation -- 62. Peptide receptors in sea urchin sperm flagella.".
• catalog tableOfContents "Part VIII. Assay and isolation of flagellar enzymes. 63. Assaying protein phosphatases in sperm and flagella -- 64. Flagellar adenylyl cyclases in Chlamydomonas -- 65. Isolation and characterization of sea urchin flagellar creatine kinase -- 66. Isolation and characterization of protein kinases of Paramecium cilia -- 67. Isolation of inner- and outer-arm dyneins -- 68. Separation of dynein species by high-pressure liquid chromatography -- 69. Reconstitution of dynein arms in vitro -- 70. Inhibitors of the dynein ATPase and ciliary of flagellar motility -- 71. Vanadate-mediated photolysis of dynein heavy chains -- 72. Modified nucleotides as probes for dynein.".
• catalog title "Cilia and flagella / edited by William Dentler and George Witman.".
• catalog type "Handbooks, manuals, etc. fast".
• catalog type "text".