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- catalog alternative "Yeast artificial chromosome libraries".
- catalog contributor b9437465.
- catalog contributor b9437466.
- catalog created "c1994.".
- catalog date "1994".
- catalog date "c1994.".
- catalog dateCopyrighted "c1994.".
- catalog description "(cont) Concentration with a filter unit (up to 600 kb remains intact) -- Microinjection of oocytes for generation of transgenic mice -- Fluorescent in situ hybridization to metaphase chromosomes with YACs -- Current fluorescent in situ hybridization (FISH) -- Implications of FISH for genome mapping -- FISH using YACs -- YAC breeding to reconstitute large loci -- Materials and methods -- Growth media.".
- catalog description "Includes bibliographical references and index.".
- catalog description "Vectors and host strains for cloning and modification of yeast artificial chromosomes -- YAC cloning vectors -- Accessory vectors for modification of YACs -- Host strains for cloning and manipulation of YACs -- Yeast spheroplast transformation with large DNA -- Aqueous transformations -- Isolation of high molecular weight yeast DNA in agarose plugs -- Inverse PCR of the ends of YAC inserts -- Inverse PCR for identifying intron/exon boundaries -- Subcloning YAC fragments into a lambda vector -- Transfer of YAC DNA into mammalian cells and transgenic mice -- Mammalian selectable markers -- Fusion of yeast spheroplasts with mammalian cells -- Solutions -- Preparation of yeast spheroplasts -- Fusion protocol -- Transformation with total yeast DNA -- Solutions -- Preparation of high molecular weight yeast DNA by centrifugation through a sucrose gradient -- Lipofectin-mediated DNA transfer -- Transformation with gel-purified YAC DNA -- Preparation of YAC DNA by agarasing a gel slice -- Microinjection into tissue-culture cells -- Lipofection into mammalian cells.".
- catalog extent "xx, 211 p. :".
- catalog identifier "0716770148".
- catalog isPartOf "UWBC biotechnical resource series".
- catalog issued "1994".
- catalog issued "c1994.".
- catalog language "eng".
- catalog publisher "New York : W.H. Freeman and Co.,".
- catalog subject "1993 A-941".
- catalog subject "589.2/330415/0724 20".
- catalog subject "Biotechnology.".
- catalog subject "Chromosomes Catalogs.".
- catalog subject "Chromosomes classification.".
- catalog subject "Chromosomes, Artificial, Yeast.".
- catalog subject "Genetic Engineering.".
- catalog subject "TP 248.27.Y43 Y12 1994".
- catalog subject "TP248.27.Y43 Y33 1994".
- catalog subject "Yeast fungi Biotechnology.".
- catalog subject "Yeast fungi Genetic engineering.".
- catalog subject "Yeasts genetics.".
- catalog tableOfContents "(cont) Concentration with a filter unit (up to 600 kb remains intact) -- Microinjection of oocytes for generation of transgenic mice -- Fluorescent in situ hybridization to metaphase chromosomes with YACs -- Current fluorescent in situ hybridization (FISH) -- Implications of FISH for genome mapping -- FISH using YACs -- YAC breeding to reconstitute large loci -- Materials and methods -- Growth media.".
- catalog tableOfContents "Vectors and host strains for cloning and modification of yeast artificial chromosomes -- YAC cloning vectors -- Accessory vectors for modification of YACs -- Host strains for cloning and manipulation of YACs -- Yeast spheroplast transformation with large DNA -- Aqueous transformations -- Isolation of high molecular weight yeast DNA in agarose plugs -- Inverse PCR of the ends of YAC inserts -- Inverse PCR for identifying intron/exon boundaries -- Subcloning YAC fragments into a lambda vector -- Transfer of YAC DNA into mammalian cells and transgenic mice -- Mammalian selectable markers -- Fusion of yeast spheroplasts with mammalian cells -- Solutions -- Preparation of yeast spheroplasts -- Fusion protocol -- Transformation with total yeast DNA -- Solutions -- Preparation of high molecular weight yeast DNA by centrifugation through a sucrose gradient -- Lipofectin-mediated DNA transfer -- Transformation with gel-purified YAC DNA -- Preparation of YAC DNA by agarasing a gel slice -- Microinjection into tissue-culture cells -- Lipofection into mammalian cells.".
- catalog title "YAC libraries : a user's guide / edited by David L. Nelson, Bernard H. Brownstein.".
- catalog title "Yeast artificial chromosome libraries".
- catalog type "Catalogs. fast".
- catalog type "text".