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- catalog abstract "This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization.".
- catalog contributor b9667191.
- catalog contributor b9667192.
- catalog contributor b9667193.
- catalog created "c1996.".
- catalog date "1996".
- catalog date "c1996.".
- catalog dateCopyrighted "c1996.".
- catalog description "1. Preparation for Protein Isolation -- 2. Protein Extraction and Solubilization -- 3. Protein Concentration Determination -- 4. Concentrating Protein Solutions -- 5. Gel Electrophoresis Under Denaturing Conditions -- 6. Gel Electrophoresis Under Nondenaturing Conditions -- 7. Isoelectric Focusing and Two-Dimensional Gel Electrophoresis -- 8. Immunoblotting -- 9. Ion Exchange Chromatography -- 10. Gel Filtration Chromatography -- 11. Affinity Chromatography -- 12. Hanging Drop Crystallization -- Appendix 1: Molecular Weights of Commonly Used Chemicals -- Appendix 2: Molecular Weights and Isoelectric Points of Selected Proteins -- Appendix 3: Ammonium Sulfate Precipitation Table -- Appendix 4: Spectrophotometer Linearity -- Appendix 5: Suppliers and Addresses.".
- catalog description "Includes bibliographical references and index.".
- catalog description "This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization.".
- catalog extent "xvi, 415 p. :".
- catalog hasFormat "Protein methods.".
- catalog identifier "0471118370 (cloth : acid₋free paper)".
- catalog isFormatOf "Protein methods.".
- catalog issued "1996".
- catalog issued "c1996.".
- catalog language "eng".
- catalog publisher "New York : Wiley-Liss,".
- catalog relation "Protein methods.".
- catalog subject "1996 G-763".
- catalog subject "574.19/245 20".
- catalog subject "Chromatography methods Laboratory Manuals.".
- catalog subject "Electrophoresis, Agar Gel methods laboratory metods.".
- catalog subject "Electrophoresis, Gel, Two-Dimensional methods Laboratory Manuals.".
- catalog subject "Electrophoresis, Gel, Two-Dimensional methods laboratory methods.".
- catalog subject "Immunoblotting methods laboratory manuals.".
- catalog subject "Isoelectric Focusing methods laboratory manuals.".
- catalog subject "Proteins Purification.".
- catalog subject "Proteins Research Methodology.".
- catalog subject "Proteins analysis laboratory manuals.".
- catalog subject "Proteins isolation & purification Laboratory Manuals.".
- catalog subject "Proteins isolation & purification laboratory methods.".
- catalog subject "QP551 .B69 1996".
- catalog subject "QU 25 B691p 1996".
- catalog tableOfContents "1. Preparation for Protein Isolation -- 2. Protein Extraction and Solubilization -- 3. Protein Concentration Determination -- 4. Concentrating Protein Solutions -- 5. Gel Electrophoresis Under Denaturing Conditions -- 6. Gel Electrophoresis Under Nondenaturing Conditions -- 7. Isoelectric Focusing and Two-Dimensional Gel Electrophoresis -- 8. Immunoblotting -- 9. Ion Exchange Chromatography -- 10. Gel Filtration Chromatography -- 11. Affinity Chromatography -- 12. Hanging Drop Crystallization -- Appendix 1: Molecular Weights of Commonly Used Chemicals -- Appendix 2: Molecular Weights and Isoelectric Points of Selected Proteins -- Appendix 3: Ammonium Sulfate Precipitation Table -- Appendix 4: Spectrophotometer Linearity -- Appendix 5: Suppliers and Addresses.".
- catalog title "Protein methods / Daniel M. Bollag, Michael D. Rozycki, Stuart J. Edelstein.".
- catalog type "text".