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catalog contributor b10004442.
catalog created "c1995.".
catalog date "1995".
catalog date "c1995.".
catalog dateCopyrighted "c1995.".
catalog description "Includes bibliographical references and index.".
catalog description "Mitochondrial nucleic acid purification and analysis / Stefan Binder -- In organello protein synthesis / Lutz Grohmann -- Separation of plant proteins by electrophoresis / Peter R. Shewry, Arthur S. Tatham, Roger J. Fido -- Western blotting analysis / Roger J. Fido, Arthur S. Tatham, Peter R. Shewry -- ELISA detection of foreign proteins / E.N. Mills, Geoffrey W. Plumb, Michael R. Morgan -- Immunocytochemical localization of proteins / John Davies.".
catalog description "Nuclear "run-on" transcription assays / John Gatehouse, Andrew J. Thompson -- RNase A/T1 protection assay / Craig G. Simpson, John W. Brown -- Primer extension assay / Craig G. Simpson, John W. Brown -- Applications of RT-PCR / Craig G. Simpson, John W. Brown -- In vitro transcription of Class II promoters in higher plants / Richard Cooke, Paul Penon -- Analysis of plant gene expression by reverse transcription-pcr / Mark Bell -- In situ hybridization to plant tissue sections / Shirley R. Burgess -- Xenopus oocytes as a heterologous expression system / Frederica L. Theodoulou, Anthony J. Miller -- Heterologous expression in yeast / Laurence J. Trueman -- The isolation of intact chloroplasts / Johnathan A. Napier, Simon A. Barnes -- In vitro protein import by isolated chloroplasts / Johnathan A. Napier -- Targeting of foreign proteins to the chloroplast / Johnathan A. Napier -- Isolation of mitochondria / Stefan Binder, Lutz Grohmann -- ".
catalog description "Tools for expressing foreign genes in plants / François Guerineau -- Introduction of cloning plasmids into Agrobacterium tumefaciens / Charles H. Shaw -- Leaf disk transformation using Agrobacterium tumefaciens : expression of heterologous genes in tobacco / Patrick Gallois, Paulo Marinho -- Agobacterium rhizogenes as a vector for transforming higher plants : application in Lotus corniculatus transformation / Jens Stougaard -- Agrobacterium-mediated transformation of Arabidopsis thaliana : application in T-DNA tagging / Jennifer F. Topping, Wenbin Wei, Michael C. Clarke, Paul Muskett, Keith Lindsey -- Agrobacterium-mediated transfer of geminiviruses to plant tissues / Margaret I. Boulton -- Stable transformation of barley via direct DNA uptake : electroporation- and PEG-mediated protoplast transformation / Paul A. Lazzeri -- Gene transfer into plant protoplasts by electroporation / Heddwyn Jones -- ".
catalog description "Transformation of cereals by microprojectile bombardment of immature inflorescence and scutellum tissues / Pilar Barcelo, Paul A. Lazzeri -- The [beta]-glucuronidase (gus) reporter gene system : gene fusions : spectrophotometric, fluorometric, and histochemical detection / Gillian A. Hull, Martine Devic -- Chloramphenicol acetyl transferase assay / Michael R. Davey, Nigel W. Blackhall, J. Brian Power -- NPTII assays for measuring gene expression and enzyme activity in transgenic plants / Ian S. Curtis, J. Brian Power, Michael R. Davey -- Gene characterization by Southern analysis / Paolo A. Sabelli, Peter R. Shewry -- Isolation and characterization of plant genomic DNA sequences via (inverse) PCR amplification / Remko Offringa, Frederique Lee -- Isolation of whole cell (total) RNA / Rod J. Scott -- Poly(A)+ RNA Isolation / Rod J. Scott -- In vitro translation / Rod J. Scott -- Northern analysis and nucleic acid probes / Paolo A. Sabelli, Peter R. Shewry -- ".
catalog extent "xiii, 466 p. :".
catalog identifier "089603321X (alk. paper)".
catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 49.".
catalog isPartOf "Methods in molecular biology ; 49".
catalog issued "1995".
catalog issued "c1995.".
catalog language "eng".
catalog publisher "Totowa, N.J. : Humana Press,".
catalog subject "581.87/322/0724 20".
catalog subject "Crops Genetic engineering Laboratory manuals.".
catalog subject "Crops, Agricultural.".
catalog subject "Gene Expression Regulation, Plant Laboratory manuals.".
catalog subject "Plant gene expression Laboratory manuals.".
catalog subject "Plant genetic engineering Laboratory manuals.".
catalog subject "Plants, Transgenic laboratory manuals.".
catalog subject "QK 981.5".
catalog subject "QK981.5 .P567 1995".
catalog tableOfContents "Mitochondrial nucleic acid purification and analysis / Stefan Binder -- In organello protein synthesis / Lutz Grohmann -- Separation of plant proteins by electrophoresis / Peter R. Shewry, Arthur S. Tatham, Roger J. Fido -- Western blotting analysis / Roger J. Fido, Arthur S. Tatham, Peter R. Shewry -- ELISA detection of foreign proteins / E.N. Mills, Geoffrey W. Plumb, Michael R. Morgan -- Immunocytochemical localization of proteins / John Davies.".
catalog tableOfContents "Nuclear "run-on" transcription assays / John Gatehouse, Andrew J. Thompson -- RNase A/T1 protection assay / Craig G. Simpson, John W. Brown -- Primer extension assay / Craig G. Simpson, John W. Brown -- Applications of RT-PCR / Craig G. Simpson, John W. Brown -- In vitro transcription of Class II promoters in higher plants / Richard Cooke, Paul Penon -- Analysis of plant gene expression by reverse transcription-pcr / Mark Bell -- In situ hybridization to plant tissue sections / Shirley R. Burgess -- Xenopus oocytes as a heterologous expression system / Frederica L. Theodoulou, Anthony J. Miller -- Heterologous expression in yeast / Laurence J. Trueman -- The isolation of intact chloroplasts / Johnathan A. Napier, Simon A. Barnes -- In vitro protein import by isolated chloroplasts / Johnathan A. Napier -- Targeting of foreign proteins to the chloroplast / Johnathan A. Napier -- Isolation of mitochondria / Stefan Binder, Lutz Grohmann -- ".
catalog tableOfContents "Tools for expressing foreign genes in plants / François Guerineau -- Introduction of cloning plasmids into Agrobacterium tumefaciens / Charles H. Shaw -- Leaf disk transformation using Agrobacterium tumefaciens : expression of heterologous genes in tobacco / Patrick Gallois, Paulo Marinho -- Agobacterium rhizogenes as a vector for transforming higher plants : application in Lotus corniculatus transformation / Jens Stougaard -- Agrobacterium-mediated transformation of Arabidopsis thaliana : application in T-DNA tagging / Jennifer F. Topping, Wenbin Wei, Michael C. Clarke, Paul Muskett, Keith Lindsey -- Agrobacterium-mediated transfer of geminiviruses to plant tissues / Margaret I. Boulton -- Stable transformation of barley via direct DNA uptake : electroporation- and PEG-mediated protoplast transformation / Paul A. Lazzeri -- Gene transfer into plant protoplasts by electroporation / Heddwyn Jones -- ".
catalog tableOfContents "Transformation of cereals by microprojectile bombardment of immature inflorescence and scutellum tissues / Pilar Barcelo, Paul A. Lazzeri -- The [beta]-glucuronidase (gus) reporter gene system : gene fusions : spectrophotometric, fluorometric, and histochemical detection / Gillian A. Hull, Martine Devic -- Chloramphenicol acetyl transferase assay / Michael R. Davey, Nigel W. Blackhall, J. Brian Power -- NPTII assays for measuring gene expression and enzyme activity in transgenic plants / Ian S. Curtis, J. Brian Power, Michael R. Davey -- Gene characterization by Southern analysis / Paolo A. Sabelli, Peter R. Shewry -- Isolation and characterization of plant genomic DNA sequences via (inverse) PCR amplification / Remko Offringa, Frederique Lee -- Isolation of whole cell (total) RNA / Rod J. Scott -- Poly(A)+ RNA Isolation / Rod J. Scott -- In vitro translation / Rod J. Scott -- Northern analysis and nucleic acid probes / Paolo A. Sabelli, Peter R. Shewry -- ".
catalog title "Plant gene transfer and expression protocols / edited by Heddwyn Jones.".
catalog type "Handbooks, manuals, etc. fast".
catalog type "text".