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• catalog abstract "This practical compendium provides clinical scientists with an essential guide to the basic techniques of molecular medicine. It serves as a laboratory manual and a source of reference. It is suitable for those wishing to perform basic semi-quantitative experiments such as Northern or Southern blots and also those wishing to undertake more specialised genetic manipulations such as gene cloning, expression and creation of DNA libraries. It will give clinical scientists a unique insight into the potential of these techniques.".
• catalog contributor b10038004.
• catalog contributor b10038005.
• catalog contributor b10038006.
• catalog created "c1996.".
• catalog date "1996".
• catalog date "c1996.".
• catalog dateCopyrighted "c1996.".
• catalog description "11. Assessing proteins and nucleic acids quantitatively and qualitatively by gel fractionation and related techniques -- 12. Extraction of protein, western blotting, special protein assays -- RIA and ELISA -- 13. Extraction of RNA and northern blot and RT PCR -- 14. Extraction of mammalian DNA, PCR, Southern blot and dot blot -- 15. Production of primers/probes J. Jankowski and F. Bedford -- 16. Molecular cytogenetic analysis of tumours and cell lines and its application in biology and medicine -- 17. Cytogenetics, FISH, allelle loss S. Jhanwar -- Analysis of DNA sequence polymorphisms and screening for point mutations A. Wright and J. Prosser -- 18. D. Gene manipulation -- 19. Molecular level -- 20. Basic cloning techniques -- 21. Purification of bacterial, plasmid or phage DNA cloning in prokaryotes and construction of recombinant DNA molecules S. Legon -- Creation and screening of cDNA libraries -- 22. A practical approach to cloning J. Goldenring and I. Modlin -- 23. Identification and isolation of novel DNA and cDna species F. Bedford and J. Jankowski -- 24. Manual and automated DNA and RNA sequencing M. Jones -- 25. Assessment of gene expression N. Bates and H. Hurst -- 26. Cellular level -- 27. Delivery of DNA into cells -- 28. Production of competent cells and cell transfection S. Castledon and R. Vile -- 29. Antisense RNA technology -- (Oligodeoxyribonucleotides, ribozymes and retroviruses) P.L. Iverson and I. Baysever -- 30. IV. Trouble-shooting: (i) Microbial contamination during cell culture K. Henderson.".
• catalog description "31.(ii) Safety aspects of genetic manipulation work S. Legon -- 32. (iii) tissue storage and preparation J. Jankowski -- 33. (iv) Failed experiments -- general guidance J. Jankowski -- 34. (v) Selection of adequate controls J. Jankowski -- 35. (vi) Quality control J. Jankowski -- 36. V. Gene manipulation and applications to molecular medicine: 37. Inherited genetic disease and developmental disorders P. Buss -- 38. Oncology of solid tumours J. Jankowski -- 39. Oncology of haematogenous cancers S. Ridge -- 40. genetic therapies K. Sikora -- VI. Appendices: A. Selected bibliography -- B. Commercial suppliers -- 41. Equipment (quantitative gel analysis and gene manipulation) -- 42. Consumables -- 43. Solutions.".
• catalog description "Includes bibliographical references.".
• catalog description "Part I. Introduction, Background and Essential Theory: 1. Introduction and applications J. Jankowski -- 2. History of molecular genetics R. Chinery -- 3. Molecules of gene expression (protein, RNA and DNA) R. Chinery -- Part II. Molecular Cell Tools: 4. Tissue analysis and morphological characterisation -- 5. Cellular analysis and cell culture -- 6. Chromosomal and molecular analysis -- 7. Gene manipulation F. Bedford and J. Jankowski -- Part III. Molecular Cell Techniques: 8. Cellular analysis -- Initiation and propagation of cultured cell lines I. Freshney -- Characterisation of cultured cell cines S. Kirkland and K. Henderson -- Cytoxicity assays in vitro R. Phillips and B. Boullier -- The production of monoclonal and polyclonal antibodies using synthetic peptides as immunogens T. RajKumar and B. Gullick -- 9. Tissue analysis -- Modern morphological techniques in molecular cell analysis (Immunocytochemistry autoradiography in situ hybridisation) L. Gordon and A. Bishop -- An overview of methodologies for assessing proliferation and cell death R. Dover -- 10. Chromosomal and molecular assays.".
• catalog description "This practical compendium provides clinical scientists with an essential guide to the basic techniques of molecular medicine. It serves as a laboratory manual and a source of reference. It is suitable for those wishing to perform basic semi-quantitative experiments such as Northern or Southern blots and also those wishing to undertake more specialised genetic manipulations such as gene cloning, expression and creation of DNA libraries. It will give clinical scientists a unique insight into the potential of these techniques.".
• catalog extent "xviii, 459 p. :".
• catalog identifier "0521473268 (hardcover)".
• catalog identifier "0521478960 (pbk.)".
• catalog isPartOf "Postgraduate medical science".
• catalog issued "1996".
• catalog issued "c1996.".
• catalog language "eng".
• catalog publisher "Cambridge ; New York : Cambridge Unversity Press,".
• catalog subject "574.87/3224 20".
• catalog subject "Gene Expression Regulation.".
• catalog subject "Genetic Techniques.".
• catalog subject "Genetic engineering Laboratory manuals.".
• catalog subject "Molecular Biology".
• catalog subject "Molecular genetics Laboratory manuals.".
• catalog subject "QH 450 C641 1996".
• catalog subject "QH442 .C565 1996".
• catalog tableOfContents "11. Assessing proteins and nucleic acids quantitatively and qualitatively by gel fractionation and related techniques -- 12. Extraction of protein, western blotting, special protein assays -- RIA and ELISA -- 13. Extraction of RNA and northern blot and RT PCR -- 14. Extraction of mammalian DNA, PCR, Southern blot and dot blot -- 15. Production of primers/probes J. Jankowski and F. Bedford -- 16. Molecular cytogenetic analysis of tumours and cell lines and its application in biology and medicine -- 17. Cytogenetics, FISH, allelle loss S. Jhanwar -- Analysis of DNA sequence polymorphisms and screening for point mutations A. Wright and J. Prosser -- 18. D. Gene manipulation -- 19. Molecular level -- 20. Basic cloning techniques -- 21. Purification of bacterial, plasmid or phage DNA cloning in prokaryotes and construction of recombinant DNA molecules S. Legon -- Creation and screening of cDNA libraries -- 22. A practical approach to cloning J. Goldenring and I. Modlin -- 23. Identification and isolation of novel DNA and cDna species F. Bedford and J. Jankowski -- 24. Manual and automated DNA and RNA sequencing M. Jones -- 25. Assessment of gene expression N. Bates and H. Hurst -- 26. Cellular level -- 27. Delivery of DNA into cells -- 28. Production of competent cells and cell transfection S. Castledon and R. Vile -- 29. Antisense RNA technology -- (Oligodeoxyribonucleotides, ribozymes and retroviruses) P.L. Iverson and I. Baysever -- 30. IV. Trouble-shooting: (i) Microbial contamination during cell culture K. Henderson.".
• catalog tableOfContents "31.(ii) Safety aspects of genetic manipulation work S. Legon -- 32. (iii) tissue storage and preparation J. Jankowski -- 33. (iv) Failed experiments -- general guidance J. Jankowski -- 34. (v) Selection of adequate controls J. Jankowski -- 35. (vi) Quality control J. Jankowski -- 36. V. Gene manipulation and applications to molecular medicine: 37. Inherited genetic disease and developmental disorders P. Buss -- 38. Oncology of solid tumours J. Jankowski -- 39. Oncology of haematogenous cancers S. Ridge -- 40. genetic therapies K. Sikora -- VI. Appendices: A. Selected bibliography -- B. Commercial suppliers -- 41. Equipment (quantitative gel analysis and gene manipulation) -- 42. Consumables -- 43. Solutions.".
• catalog tableOfContents "Part I. Introduction, Background and Essential Theory: 1. Introduction and applications J. Jankowski -- 2. History of molecular genetics R. Chinery -- 3. Molecules of gene expression (protein, RNA and DNA) R. Chinery -- Part II. Molecular Cell Tools: 4. Tissue analysis and morphological characterisation -- 5. Cellular analysis and cell culture -- 6. Chromosomal and molecular analysis -- 7. Gene manipulation F. Bedford and J. Jankowski -- Part III. Molecular Cell Techniques: 8. Cellular analysis -- Initiation and propagation of cultured cell lines I. Freshney -- Characterisation of cultured cell cines S. Kirkland and K. Henderson -- Cytoxicity assays in vitro R. Phillips and B. Boullier -- The production of monoclonal and polyclonal antibodies using synthetic peptides as immunogens T. RajKumar and B. Gullick -- 9. Tissue analysis -- Modern morphological techniques in molecular cell analysis (Immunocytochemistry autoradiography in situ hybridisation) L. Gordon and A. Bishop -- An overview of methodologies for assessing proliferation and cell death R. Dover -- 10. Chromosomal and molecular assays.".
• catalog title "Clinical gene analysis and manipulation : tools, techniques, and troubleshooting / by Janusz A.Z. Jankowski, editor ; Julia Polak, deputy editor ; foreword, Sir David Weatherall.".
• catalog type "Handbooks, manuals, etc. fast".
• catalog type "text".