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- catalog contributor b10193773.
- catalog created "c1997.".
- catalog date "1997".
- catalog date "c1997.".
- catalog dateCopyrighted "c1997.".
- catalog description "1. Introduction -- Recombinanat gene expression protocols overview of experimental strategies for the expression of recombinant genes -- 2. Expression in E. coli -- Designing expression plasmid vectors in E. coli -- Design of bacterial hosts for Iac-based expressed vectors -- Bacteriophage gamma-based expression vectors -- Strategies for gene fusions -- Application of the E. coli trp promoter -- Expression of proteins in E. coli utilizing a dual promoter-based vector: pLACT7 -- Expression and secretion of proteins in E. coli -- Synthetic two-cistron expression system -- Expression of cDNA-encoded proteins by cell-free transcription and translation -- 3. Expression in yeast -- Yeast plasids -- Inducible expression cassettes in yeast: GAL4 -- Inducible expression cassettes in yeast: ADH2 -- Constitutive expression vectors: PGK -- 4. Viral expression systems -- Design and construction of recombinant vaccinia viruses -- A simplified method of screening for isolation of recombinant vaccinia virus -- Design of retroviral expression vectors -- Use of defective herpes-derived plasmid vectors -- 5. Expression in nonmammalian higher eukaryotes -- Use of baculovirus expression vectors -- Expression of exogenous genes in Xenopus oocytes, eggs, and embryos -- Analysis of heterologous gene expression in Xenopus blastomeres -- 6. Expression in mammalian cells -- Overview of vector design for mammalian gene expression".
- catalog description "Experimental strategies in efficient transfection of mammalian cells: calcium phosphate and DAAE-dextran -- Experimental strategies in efficient transfection of mammalian cells: electroporation -- Highly effective delivery of foreign DNA to atherent cells via polybrene/DMSO-assisted gene transfer -- Selection of transfected cells: magnetic affinity cell sorting -- Selection of transfected cells and coamplification of transfected genes -- Optimization of growth, viability, and specific productivity for expression of recombinant proteins in mammalian cells -- 7. Expression in transgenic organisms -- 8. Expression in Plants.".
- catalog description "Includes bibliographical references and index.".
- catalog extent "xx, 521 p. :".
- catalog identifier "0896033333 (alk. paper)".
- catalog identifier "0896034801 (hardcover)".
- catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 62.".
- catalog isPartOf "Methods in molecular biology ; v. 62".
- catalog issued "1997".
- catalog issued "c1997.".
- catalog language "eng".
- catalog publisher "Totowa, N.J. : Humana Press,".
- catalog subject "572.8/77 21".
- catalog subject "Gene Expression".
- catalog subject "Genetic Engineering".
- catalog subject "Genetic recombination Laboratory manuals.".
- catalog subject "QH 443 R3108 1997".
- catalog subject "QH443 .R36 1997".
- catalog subject "Recombination, Genetic".
- catalog subject "W1 ME9616J v.62 1997".
- catalog tableOfContents "1. Introduction -- Recombinanat gene expression protocols overview of experimental strategies for the expression of recombinant genes -- 2. Expression in E. coli -- Designing expression plasmid vectors in E. coli -- Design of bacterial hosts for Iac-based expressed vectors -- Bacteriophage gamma-based expression vectors -- Strategies for gene fusions -- Application of the E. coli trp promoter -- Expression of proteins in E. coli utilizing a dual promoter-based vector: pLACT7 -- Expression and secretion of proteins in E. coli -- Synthetic two-cistron expression system -- Expression of cDNA-encoded proteins by cell-free transcription and translation -- 3. Expression in yeast -- Yeast plasids -- Inducible expression cassettes in yeast: GAL4 -- Inducible expression cassettes in yeast: ADH2 -- Constitutive expression vectors: PGK -- 4. Viral expression systems -- Design and construction of recombinant vaccinia viruses -- A simplified method of screening for isolation of recombinant vaccinia virus -- Design of retroviral expression vectors -- Use of defective herpes-derived plasmid vectors -- 5. Expression in nonmammalian higher eukaryotes -- Use of baculovirus expression vectors -- Expression of exogenous genes in Xenopus oocytes, eggs, and embryos -- Analysis of heterologous gene expression in Xenopus blastomeres -- 6. Expression in mammalian cells -- Overview of vector design for mammalian gene expression".
- catalog tableOfContents "Experimental strategies in efficient transfection of mammalian cells: calcium phosphate and DAAE-dextran -- Experimental strategies in efficient transfection of mammalian cells: electroporation -- Highly effective delivery of foreign DNA to atherent cells via polybrene/DMSO-assisted gene transfer -- Selection of transfected cells: magnetic affinity cell sorting -- Selection of transfected cells and coamplification of transfected genes -- Optimization of growth, viability, and specific productivity for expression of recombinant proteins in mammalian cells -- 7. Expression in transgenic organisms -- 8. Expression in Plants.".
- catalog title "Recombinant gene expression protocols / edited by Rocky S. Tuan.".
- catalog type "Laboratory Manuals".
- catalog type "text".