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• catalog abstract "Designed for both novice and experienced researchers, this volume presents time-tested techniques to cover all aspects of nitric oxide synthase and metabolism.".
• catalog contributor b10705830.
• catalog created "c1998.".
• catalog date "1998".
• catalog date "c1998.".
• catalog dateCopyrighted "c1998.".
• catalog description "Designed for both novice and experienced researchers, this volume presents time-tested techniques to cover all aspects of nitric oxide synthase and metabolism.".
• catalog description "Determination of NOS activity using cyclic-GMP formation / Suzanne G. Laychock -- Determination of NO with a Clark-type electrode / Kurt Schmidt and Bernd Mayer -- The measurement of NO in biological systems using chemiluminescence / Evangelos D. Michelakis and Stephen L. Archer -- Measurement of NO using electron paramagnetic resonance / S. Tsuyoshi Ohnishi -- Measurement of eNOS and iNOS mRNA expression using reverse transcription polymerase chain reaction / Norbert Reiling, Artur J. Ulmer, Sunna Hauschildt -- Measurement of NOS mRNA by Northern blotting and the ribonuclease-protection assay / Paloma Martín-Sanz and Lisardo Boscá -- Detection of NOS isoforms by Western-blot analysis / Fiona S. Smith and Michael A. Titheradge --Immunohistochemical localization of NOS isoforms / Victoria Cattell and Karen Mosley -- A practical protocol for the demonstration of NOS using in-situ hybridization / G. Michael Taylor and H. Terence Cook -- ".
• catalog description "Enzymology of nitric oxide synthases / Benjamin Hemmens and Bernd Mayer -- Purification of the inducible nitric oxide synthase ; Purification of the constitutive nitric oxide synthase / Edward P. Garvey -- Cloning and expression of human inducible nitric oxide synthase / Mark L. Johnson, Richard A. Shapiro, Timothy R. Billiar -- Cloning and expression of human eNOS and nNos using the baculovirus-insect cell system / Ian G. Charles, Neale Foxwell, Ann Chubb -- Assay of NOS activity by the measurement of conversion of oxyhemoglobin to methemoglobin by NO / Mark Salter and Richard G. Knowles -- Measurement of NOS activity by conversion of radiolabeled arginine to citrulline using ion-exchange separation / Richard G. Knowles and Mark Salter -- Radiochemical measurement of NOS activity by conversion of [¹⁴C]L-arginine to citrulline using HPLC separation / James M. Cunningham and Richard C. Rayne -- The enzymatic measurement of nitrate and nitrite / Michael A. Titheradge -- ".
• catalog description "Includes bibliographical references and index.".
• catalog description "Measurement of DNA damage using the comet assay / Steven Thomas [and others] -- Methods for the study of NO-induced apoptosis in cultured cells / Anne C. Loweth and Noel G. Morgan.".
• catalog description "Use of NO donors in biological systems / Jayne M. Tullett and Daryl D. Rees -- Making and working with peroxynitrite / Roger White [and others] -- The use of NO gas in biological systems / Neil Hogg and B. Kalyanaraman -- A microtiter-plate assay of human NOS isoforms / John Dawson and Richard G. Knowles -- Use of arginine analogs as inhibitors of nitric oxide synthase in rat-aortic rings / Rachel J. Russell -- Measurement of biopterin and the use of inhibitors of biopterin biosynthesis / Kazuyuki Hatakeyama -- A sensitive assay for the enzymatic activity of GTP cyclohydrolase I / Kazuyuki Hatakeyama and Toshie Yoneyama -- Simultaneous measurement of mitochondrial function and NO / Ignacio Lizasoain and María A. Moro -- Release of NO from donor compounds : a mathematical model for calculation of NO concentrations in the presence of oxygen / Kurt Schmidt [and others] -- The determination of nitrotyrosine residues in proteins / Andrew J. Gow [and others] -- ".
• catalog extent "xi, 324 p. :".
• catalog hasFormat "Nitric oxide protocols.".
• catalog identifier "0896035379".
• catalog isFormatOf "Nitric oxide protocols.".
• catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 100.".
• catalog isPartOf "Methods in molecular biology ; 100".
• catalog issued "1998".
• catalog issued "c1998.".
• catalog language "eng".
• catalog publisher "Totowa, N.J. : Humana Press,".
• catalog relation "Nitric oxide protocols.".
• catalog subject "Nitric Oxide isolation & purification Laboratory Manuals.".
• catalog subject "Nitric Oxide metabolism Laboratory Manuals.".
• catalog subject "Nitric Oxide metabolism.".
• catalog subject "Nitric oxide Metabolism.".
• catalog subject "Nitric oxide Synthesis.".
• catalog subject "QD181.N1 N58 1998".
• catalog subject "QP535.N1 N557 1998".
• catalog subject "QV 25 N731 1998".
• catalog subject "W1 ME9616J v.100 1998".
• catalog tableOfContents "Determination of NOS activity using cyclic-GMP formation / Suzanne G. Laychock -- Determination of NO with a Clark-type electrode / Kurt Schmidt and Bernd Mayer -- The measurement of NO in biological systems using chemiluminescence / Evangelos D. Michelakis and Stephen L. Archer -- Measurement of NO using electron paramagnetic resonance / S. Tsuyoshi Ohnishi -- Measurement of eNOS and iNOS mRNA expression using reverse transcription polymerase chain reaction / Norbert Reiling, Artur J. Ulmer, Sunna Hauschildt -- Measurement of NOS mRNA by Northern blotting and the ribonuclease-protection assay / Paloma Martín-Sanz and Lisardo Boscá -- Detection of NOS isoforms by Western-blot analysis / Fiona S. Smith and Michael A. Titheradge --Immunohistochemical localization of NOS isoforms / Victoria Cattell and Karen Mosley -- A practical protocol for the demonstration of NOS using in-situ hybridization / G. Michael Taylor and H. Terence Cook -- ".
• catalog tableOfContents "Enzymology of nitric oxide synthases / Benjamin Hemmens and Bernd Mayer -- Purification of the inducible nitric oxide synthase ; Purification of the constitutive nitric oxide synthase / Edward P. Garvey -- Cloning and expression of human inducible nitric oxide synthase / Mark L. Johnson, Richard A. Shapiro, Timothy R. Billiar -- Cloning and expression of human eNOS and nNos using the baculovirus-insect cell system / Ian G. Charles, Neale Foxwell, Ann Chubb -- Assay of NOS activity by the measurement of conversion of oxyhemoglobin to methemoglobin by NO / Mark Salter and Richard G. Knowles -- Measurement of NOS activity by conversion of radiolabeled arginine to citrulline using ion-exchange separation / Richard G. Knowles and Mark Salter -- Radiochemical measurement of NOS activity by conversion of [¹⁴C]L-arginine to citrulline using HPLC separation / James M. Cunningham and Richard C. Rayne -- The enzymatic measurement of nitrate and nitrite / Michael A. Titheradge -- ".
• catalog tableOfContents "Measurement of DNA damage using the comet assay / Steven Thomas [and others] -- Methods for the study of NO-induced apoptosis in cultured cells / Anne C. Loweth and Noel G. Morgan.".
• catalog tableOfContents "Use of NO donors in biological systems / Jayne M. Tullett and Daryl D. Rees -- Making and working with peroxynitrite / Roger White [and others] -- The use of NO gas in biological systems / Neil Hogg and B. Kalyanaraman -- A microtiter-plate assay of human NOS isoforms / John Dawson and Richard G. Knowles -- Use of arginine analogs as inhibitors of nitric oxide synthase in rat-aortic rings / Rachel J. Russell -- Measurement of biopterin and the use of inhibitors of biopterin biosynthesis / Kazuyuki Hatakeyama -- A sensitive assay for the enzymatic activity of GTP cyclohydrolase I / Kazuyuki Hatakeyama and Toshie Yoneyama -- Simultaneous measurement of mitochondrial function and NO / Ignacio Lizasoain and María A. Moro -- Release of NO from donor compounds : a mathematical model for calculation of NO concentrations in the presence of oxygen / Kurt Schmidt [and others] -- The determination of nitrotyrosine residues in proteins / Andrew J. Gow [and others] -- ".
• catalog title "Nitric oxide protocols / edited by Michael A. Titheradge.".
• catalog type "text".